Allergic airway inflammation and susceptibility to pneumococcal pneumonia in a murine model with real-time in vivo evaluation

C. I. Kang, M. S. Rouse, Robin Patel, Hirohito Kita, Young J Juhn

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15 Citations (Scopus)

Abstract

The relationship between allergic airway inflammation and pneumococcal pneumonia is not well understood. We assessed susceptibility to experimental pneumococcal pneumonia in mice with and without allergic airway inflammation. Susceptibility to pneumococcal pneumonia was evaluated by challenging mice with a bioluminescent Streptococcus pneumoniae strain after sensitization with ovalbumin (OVA), with subsequent monitoring of pneumococcal infection using real-time photonic imaging. Of 46 OVA-sensitized mice challenged with pneumococci, 13 (28%) developed imaging findings consistent with pneumococcal pneumonia. In comparison, 28 (57%) of 49 non-sensitized control mice developed pneumococcal pneumonia (P = 0·005). While none of the control group developed meningitis (0%, none of 28), two mice in the OVA-sensitized group developed meningitis (15·4%, two of 13) (P = 0·09). The mean bacterial count in the lung was significantly lower in the OVA-sensitized than the non-sensitized group (8·26 ± 0·69 versus 9·21 ± 0·67 log 10 colony-forming units (CFU)/g, P = 0·002). There was a trend towards the mean bacterial count in the spleen being higher in the OVA-sensitized versus the non-sensitized group (8·14 ± 0·89 versus 7·45 ± 1·07 log 10 CFU/g, P = 0·071). A high level of interleukin (IL)-4 in lung homogenates was associated with risk of pneumococcal infection independent of sensitization with OVA (odds ratio: 49·7, 95% confidence interval 2·92- 846·5, per increment of 1·0 pg/ml). In the murine model studied, acute allergic airway inflammation reduced susceptibility to pneumococcal pneumonia. IL-4 may increase the risk of pneumococcal pneumonia independently of allergic airway inflammation.

Original languageEnglish (US)
Pages (from-to)552-561
Number of pages10
JournalClinical and Experimental Immunology
Volume156
Issue number3
DOIs
StatePublished - Jun 2009

Fingerprint

Pneumococcal Pneumonia
Ovalbumin
Inflammation
Pneumococcal Infections
Bacterial Load
Streptococcus pneumoniae
Meningitis
Interleukin-4
Stem Cells
Optics and Photonics
Lung
Spleen
Odds Ratio
Confidence Intervals
Control Groups

Keywords

  • Allergy
  • Asthma
  • Pneumonia
  • Streptococcus pneumoniae

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy

Cite this

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title = "Allergic airway inflammation and susceptibility to pneumococcal pneumonia in a murine model with real-time in vivo evaluation",
abstract = "The relationship between allergic airway inflammation and pneumococcal pneumonia is not well understood. We assessed susceptibility to experimental pneumococcal pneumonia in mice with and without allergic airway inflammation. Susceptibility to pneumococcal pneumonia was evaluated by challenging mice with a bioluminescent Streptococcus pneumoniae strain after sensitization with ovalbumin (OVA), with subsequent monitoring of pneumococcal infection using real-time photonic imaging. Of 46 OVA-sensitized mice challenged with pneumococci, 13 (28{\%}) developed imaging findings consistent with pneumococcal pneumonia. In comparison, 28 (57{\%}) of 49 non-sensitized control mice developed pneumococcal pneumonia (P = 0·005). While none of the control group developed meningitis (0{\%}, none of 28), two mice in the OVA-sensitized group developed meningitis (15·4{\%}, two of 13) (P = 0·09). The mean bacterial count in the lung was significantly lower in the OVA-sensitized than the non-sensitized group (8·26 ± 0·69 versus 9·21 ± 0·67 log 10 colony-forming units (CFU)/g, P = 0·002). There was a trend towards the mean bacterial count in the spleen being higher in the OVA-sensitized versus the non-sensitized group (8·14 ± 0·89 versus 7·45 ± 1·07 log 10 CFU/g, P = 0·071). A high level of interleukin (IL)-4 in lung homogenates was associated with risk of pneumococcal infection independent of sensitization with OVA (odds ratio: 49·7, 95{\%} confidence interval 2·92- 846·5, per increment of 1·0 pg/ml). In the murine model studied, acute allergic airway inflammation reduced susceptibility to pneumococcal pneumonia. IL-4 may increase the risk of pneumococcal pneumonia independently of allergic airway inflammation.",
keywords = "Allergy, Asthma, Pneumonia, Streptococcus pneumoniae",
author = "Kang, {C. I.} and Rouse, {M. S.} and Robin Patel and Hirohito Kita and Juhn, {Young J}",
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T1 - Allergic airway inflammation and susceptibility to pneumococcal pneumonia in a murine model with real-time in vivo evaluation

AU - Kang, C. I.

AU - Rouse, M. S.

AU - Patel, Robin

AU - Kita, Hirohito

AU - Juhn, Young J

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N2 - The relationship between allergic airway inflammation and pneumococcal pneumonia is not well understood. We assessed susceptibility to experimental pneumococcal pneumonia in mice with and without allergic airway inflammation. Susceptibility to pneumococcal pneumonia was evaluated by challenging mice with a bioluminescent Streptococcus pneumoniae strain after sensitization with ovalbumin (OVA), with subsequent monitoring of pneumococcal infection using real-time photonic imaging. Of 46 OVA-sensitized mice challenged with pneumococci, 13 (28%) developed imaging findings consistent with pneumococcal pneumonia. In comparison, 28 (57%) of 49 non-sensitized control mice developed pneumococcal pneumonia (P = 0·005). While none of the control group developed meningitis (0%, none of 28), two mice in the OVA-sensitized group developed meningitis (15·4%, two of 13) (P = 0·09). The mean bacterial count in the lung was significantly lower in the OVA-sensitized than the non-sensitized group (8·26 ± 0·69 versus 9·21 ± 0·67 log 10 colony-forming units (CFU)/g, P = 0·002). There was a trend towards the mean bacterial count in the spleen being higher in the OVA-sensitized versus the non-sensitized group (8·14 ± 0·89 versus 7·45 ± 1·07 log 10 CFU/g, P = 0·071). A high level of interleukin (IL)-4 in lung homogenates was associated with risk of pneumococcal infection independent of sensitization with OVA (odds ratio: 49·7, 95% confidence interval 2·92- 846·5, per increment of 1·0 pg/ml). In the murine model studied, acute allergic airway inflammation reduced susceptibility to pneumococcal pneumonia. IL-4 may increase the risk of pneumococcal pneumonia independently of allergic airway inflammation.

AB - The relationship between allergic airway inflammation and pneumococcal pneumonia is not well understood. We assessed susceptibility to experimental pneumococcal pneumonia in mice with and without allergic airway inflammation. Susceptibility to pneumococcal pneumonia was evaluated by challenging mice with a bioluminescent Streptococcus pneumoniae strain after sensitization with ovalbumin (OVA), with subsequent monitoring of pneumococcal infection using real-time photonic imaging. Of 46 OVA-sensitized mice challenged with pneumococci, 13 (28%) developed imaging findings consistent with pneumococcal pneumonia. In comparison, 28 (57%) of 49 non-sensitized control mice developed pneumococcal pneumonia (P = 0·005). While none of the control group developed meningitis (0%, none of 28), two mice in the OVA-sensitized group developed meningitis (15·4%, two of 13) (P = 0·09). The mean bacterial count in the lung was significantly lower in the OVA-sensitized than the non-sensitized group (8·26 ± 0·69 versus 9·21 ± 0·67 log 10 colony-forming units (CFU)/g, P = 0·002). There was a trend towards the mean bacterial count in the spleen being higher in the OVA-sensitized versus the non-sensitized group (8·14 ± 0·89 versus 7·45 ± 1·07 log 10 CFU/g, P = 0·071). A high level of interleukin (IL)-4 in lung homogenates was associated with risk of pneumococcal infection independent of sensitization with OVA (odds ratio: 49·7, 95% confidence interval 2·92- 846·5, per increment of 1·0 pg/ml). In the murine model studied, acute allergic airway inflammation reduced susceptibility to pneumococcal pneumonia. IL-4 may increase the risk of pneumococcal pneumonia independently of allergic airway inflammation.

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