Allergen-specific in vitro cytokine production in adult patients with eosinophilic esophagitis

Kiyoshi Yamazaki, Joseph A Murray, Amindra S. Arora, Jeffery A. Alexander, Thomas Christopher Smyrk, Joseph H. Butterfield, Hirohito Kita

Research output: Contribution to journalArticle

71 Citations (Scopus)

Abstract

Although the pathogenesis of eosinophilic esophagitis (EE) likely involves hypersensitivity reactions against exogenous allergens, allergen-specific cellular immune responses have not been studied. We investigated allergen-induced cytokine production by peripheral blood mononuclear cells (PBMCs) in adult patients with EE (n=15) and healthy controls (HC; n=9). PBMCs were incubated with nine common food and environmental allergens or a nonspecific mitogen, and the levels of interleukin (IL)-5, IL-10, IL-13, and interferon-γ in the cell-free supernatants were determined. Spontaneous and mitogen-stimulated cytokine production did not differ between EE and HC. House dust mite, ragweed, Aspergillus, milk, and soy induced significantly higher IL-5 production in EE (P < 0.05). House dust mite also augmented IL-13 production in EE (P < 0.05). Furthermore, PBMCs from three EE patients without allergen-specific IgE vigorously produced IL-5 and IL-13 on allergen stimulation. Thus, immune responses in EE are characterized by enhanced production of Th2-like cytokines against both food and environmental allergens.

Original languageEnglish (US)
Pages (from-to)1934-1941
Number of pages8
JournalDigestive Diseases and Sciences
Volume51
Issue number11
DOIs
StatePublished - Nov 2006

Fingerprint

Eosinophilic Esophagitis
Allergens
Cytokines
Interleukin-13
Interleukin-5
Blood Cells
Pyroglyphidae
Mitogens
Soy Milk
Ambrosia
Food
Aspergillus
In Vitro Techniques
Cellular Immunity
Interleukin-10
Interferons
Immunoglobulin E
Hypersensitivity

Keywords

  • Allergens
  • Eosinophils
  • Esophagitis
  • Immunoglobulin E
  • Interleukin-13
  • Interleukin-5

ASJC Scopus subject areas

  • Gastroenterology

Cite this

Allergen-specific in vitro cytokine production in adult patients with eosinophilic esophagitis. / Yamazaki, Kiyoshi; Murray, Joseph A; Arora, Amindra S.; Alexander, Jeffery A.; Smyrk, Thomas Christopher; Butterfield, Joseph H.; Kita, Hirohito.

In: Digestive Diseases and Sciences, Vol. 51, No. 11, 11.2006, p. 1934-1941.

Research output: Contribution to journalArticle

Yamazaki, Kiyoshi ; Murray, Joseph A ; Arora, Amindra S. ; Alexander, Jeffery A. ; Smyrk, Thomas Christopher ; Butterfield, Joseph H. ; Kita, Hirohito. / Allergen-specific in vitro cytokine production in adult patients with eosinophilic esophagitis. In: Digestive Diseases and Sciences. 2006 ; Vol. 51, No. 11. pp. 1934-1941.
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