Allergen-induced traffic of bone marrow eosinophils, neutrophils and lymphocytes to airways

Anna Karin Johansson, Svetlana Sergejeva, Margareta Sjöstrand, James J. Lee, Jan Lötvall

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

We evaluated whether bone marrow (BM) inflammatory cells have capacity to traffic into the airways following allergen exposure in a mouse model of allergen-induced airway inflammation. We also evaluated the effect of IL-5 overexpression on (i) the production of eosinophils in BM, (ii) the accumulation of eosinophils, neutrophils and lymphocytes in blood and airways and (iii) the changes in CD34+ cell numbers in BM, blood and airways. Bromodeoxyuridine (BrdU) was used to label cells produced during the exposure period. Furthermore, CD3 splenocytes were adoptively transferred to investigate the BM inflammatory response. Allergen exposure induced traffic of BM eosinophils, neutrophils and lymphocytes to the airways and increased the number of BrdU+ eosinophils, neutrophils, lymphocytes and CD34+ cells in BALf. IL-5 overexpression enhanced the eosinophilopoiesis and increased the presence of BrdU+ eosinophils and CD34+ cells in airways and enhanced the number of CD34+ cells in BM and blood after allergen exposure. Adoptive transfer of CD3 lymphocytes overexpressing IL-5 caused increased BM eosinophilia. In conclusion, allergen exposure induces traffic of not only newly produced eosinophils but also newly produced neutrophils and lymphocytes into the airways.

Original languageEnglish (US)
Pages (from-to)3135-3145
Number of pages11
JournalEuropean Journal of Immunology
Volume34
Issue number11
DOIs
StatePublished - Nov 2004

Fingerprint

Eosinophils
Allergens
Neutrophils
Bone Marrow
Lymphocytes
Interleukin-5
Bromodeoxyuridine
Bone Marrow Cells
Adoptive Transfer
Eosinophilia
Cell Count
Inflammation

Keywords

  • CD34cells
  • CD3lymphocytes
  • Inflammation
  • NJ.1638

ASJC Scopus subject areas

  • Immunology

Cite this

Johansson, A. K., Sergejeva, S., Sjöstrand, M., Lee, J. J., & Lötvall, J. (2004). Allergen-induced traffic of bone marrow eosinophils, neutrophils and lymphocytes to airways. European Journal of Immunology, 34(11), 3135-3145. https://doi.org/10.1002/eji.200425043

Allergen-induced traffic of bone marrow eosinophils, neutrophils and lymphocytes to airways. / Johansson, Anna Karin; Sergejeva, Svetlana; Sjöstrand, Margareta; Lee, James J.; Lötvall, Jan.

In: European Journal of Immunology, Vol. 34, No. 11, 11.2004, p. 3135-3145.

Research output: Contribution to journalArticle

Johansson, AK, Sergejeva, S, Sjöstrand, M, Lee, JJ & Lötvall, J 2004, 'Allergen-induced traffic of bone marrow eosinophils, neutrophils and lymphocytes to airways', European Journal of Immunology, vol. 34, no. 11, pp. 3135-3145. https://doi.org/10.1002/eji.200425043
Johansson, Anna Karin ; Sergejeva, Svetlana ; Sjöstrand, Margareta ; Lee, James J. ; Lötvall, Jan. / Allergen-induced traffic of bone marrow eosinophils, neutrophils and lymphocytes to airways. In: European Journal of Immunology. 2004 ; Vol. 34, No. 11. pp. 3135-3145.
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