Allelic loss and mutational analysis of the DPC4 gene in esophageal adenocarcinoma

Michael Barrett, Mieke Schutte, Scott E. Kern, Brian J. Reid

Research output: Contribution to journalArticle

84 Citations (Scopus)

Abstract

DPC4, a recently cloned gene located on 18q21.1, is inactivated in almost one half of pancreatic adenocarcinomas. To determine whether DPC4 inactivation is involved in esophageal adenocarcinoma, we have analyzed aneuploid populations from biopsies of 35 patients with Barrett's esophagus who had premalignant epithelium, adenocarcinoma, or both. Sixteen of 35 patients (46%) had allelic loss at 18q21.1, including 7 patients who had only premalignant tissue present in their Barrett segment. In addition, three of four patients (75%) with 18q21.l loss in their aneuploid populations had the allelic loss present in diploid cells. Mutational analysis of DPC4 did not reveal any inactivating alterations in the gene. These data indicate that allelic losses at 18q are selected during neoplastic progression in Barrett's esophagus, but the targeted gene remains to be identified.

Original languageEnglish (US)
Pages (from-to)4351-4353
Number of pages3
JournalCancer Research
Volume56
Issue number19
StatePublished - Oct 1 1996
Externally publishedYes

Fingerprint

Loss of Heterozygosity
Adenocarcinoma
Barrett Esophagus
Aneuploidy
Genes
Diploidy
Population
Epithelium
Biopsy

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Barrett, M., Schutte, M., Kern, S. E., & Reid, B. J. (1996). Allelic loss and mutational analysis of the DPC4 gene in esophageal adenocarcinoma. Cancer Research, 56(19), 4351-4353.

Allelic loss and mutational analysis of the DPC4 gene in esophageal adenocarcinoma. / Barrett, Michael; Schutte, Mieke; Kern, Scott E.; Reid, Brian J.

In: Cancer Research, Vol. 56, No. 19, 01.10.1996, p. 4351-4353.

Research output: Contribution to journalArticle

Barrett, M, Schutte, M, Kern, SE & Reid, BJ 1996, 'Allelic loss and mutational analysis of the DPC4 gene in esophageal adenocarcinoma', Cancer Research, vol. 56, no. 19, pp. 4351-4353.
Barrett, Michael ; Schutte, Mieke ; Kern, Scott E. ; Reid, Brian J. / Allelic loss and mutational analysis of the DPC4 gene in esophageal adenocarcinoma. In: Cancer Research. 1996 ; Vol. 56, No. 19. pp. 4351-4353.
@article{8096bd7b9d6c414bac00de701be70682,
title = "Allelic loss and mutational analysis of the DPC4 gene in esophageal adenocarcinoma",
abstract = "DPC4, a recently cloned gene located on 18q21.1, is inactivated in almost one half of pancreatic adenocarcinomas. To determine whether DPC4 inactivation is involved in esophageal adenocarcinoma, we have analyzed aneuploid populations from biopsies of 35 patients with Barrett's esophagus who had premalignant epithelium, adenocarcinoma, or both. Sixteen of 35 patients (46{\%}) had allelic loss at 18q21.1, including 7 patients who had only premalignant tissue present in their Barrett segment. In addition, three of four patients (75{\%}) with 18q21.l loss in their aneuploid populations had the allelic loss present in diploid cells. Mutational analysis of DPC4 did not reveal any inactivating alterations in the gene. These data indicate that allelic losses at 18q are selected during neoplastic progression in Barrett's esophagus, but the targeted gene remains to be identified.",
author = "Michael Barrett and Mieke Schutte and Kern, {Scott E.} and Reid, {Brian J.}",
year = "1996",
month = "10",
day = "1",
language = "English (US)",
volume = "56",
pages = "4351--4353",
journal = "Journal of Cancer Research",
issn = "0099-7013",
publisher = "American Association for Cancer Research Inc.",
number = "19",

}

TY - JOUR

T1 - Allelic loss and mutational analysis of the DPC4 gene in esophageal adenocarcinoma

AU - Barrett, Michael

AU - Schutte, Mieke

AU - Kern, Scott E.

AU - Reid, Brian J.

PY - 1996/10/1

Y1 - 1996/10/1

N2 - DPC4, a recently cloned gene located on 18q21.1, is inactivated in almost one half of pancreatic adenocarcinomas. To determine whether DPC4 inactivation is involved in esophageal adenocarcinoma, we have analyzed aneuploid populations from biopsies of 35 patients with Barrett's esophagus who had premalignant epithelium, adenocarcinoma, or both. Sixteen of 35 patients (46%) had allelic loss at 18q21.1, including 7 patients who had only premalignant tissue present in their Barrett segment. In addition, three of four patients (75%) with 18q21.l loss in their aneuploid populations had the allelic loss present in diploid cells. Mutational analysis of DPC4 did not reveal any inactivating alterations in the gene. These data indicate that allelic losses at 18q are selected during neoplastic progression in Barrett's esophagus, but the targeted gene remains to be identified.

AB - DPC4, a recently cloned gene located on 18q21.1, is inactivated in almost one half of pancreatic adenocarcinomas. To determine whether DPC4 inactivation is involved in esophageal adenocarcinoma, we have analyzed aneuploid populations from biopsies of 35 patients with Barrett's esophagus who had premalignant epithelium, adenocarcinoma, or both. Sixteen of 35 patients (46%) had allelic loss at 18q21.1, including 7 patients who had only premalignant tissue present in their Barrett segment. In addition, three of four patients (75%) with 18q21.l loss in their aneuploid populations had the allelic loss present in diploid cells. Mutational analysis of DPC4 did not reveal any inactivating alterations in the gene. These data indicate that allelic losses at 18q are selected during neoplastic progression in Barrett's esophagus, but the targeted gene remains to be identified.

UR - http://www.scopus.com/inward/record.url?scp=0029759540&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0029759540&partnerID=8YFLogxK

M3 - Article

C2 - 8813122

AN - SCOPUS:0029759540

VL - 56

SP - 4351

EP - 4353

JO - Journal of Cancer Research

JF - Journal of Cancer Research

SN - 0099-7013

IS - 19

ER -