Allelic imbalance and microsatellite instability in prostatic adenocarcinoma

Julie M Cunningham, Ailin Shan, Myra J Wick, Shannon K. McDonnell, Daniel J Schaid, David J. Tester, Junqi Qian, Satoru Takahashi, Robert Brian Jenkins, David G. Bostwick, Stephen N Thibodeau

Research output: Contribution to journalArticle

163 Citations (Scopus)

Abstract

Although prostate cancer is one of the most common malignancies of males in Western countries, relatively little is known about the molecular mechanisms involved in tumor initiation and progression. Allelic loss studies have suggested the involvement of multiple tumor suppressor genes (TSGs), hut few detailed studies of all chromosomes have been performed. In an effort to localize and identify candidate TSGs, we performed allelic imbalance (AI) studies on 55 prostate cancers, using 135 polymorphic microsatellite markers. For the entire chromosome. AI ranged from a low of 0% on chromosomes 14 and 20 to a high of 71% on chromosome 8. Chromosomal regions demonstrating at least twice the background frequency of AI (ranging from 20 to 69%) included 5q, 6q, 7q, 8p. 13, 16q, 18q, and 21. In addition, AI was examined for association with a number of clinicopathological parameters. AI on chromosomes 7 and 16 were each associated with greater age at diagnosis (P = 0.009 and 0.001, respectively), and AI on chromosomes 10, 16, and 18 was associated with aneuploidy/tetraploidy (P = 0.037, 0.013, and 0.054, respectively). Furthermore, AI on chromosome 5 was associated with a higher pathological stage (P = 0.021) and on chromosome 8 and 16 with a higher Glesson score (P = 0.027 and 0.041, respectively). No tumor exhibited a phenotype of widespread microsatellite instability. These results indicate that there likely exist multiple sites harboring candidate TSG in prostate cancer, some of which may have important clinical implications, and which argue against widespread microsatellite instability.

Original languageEnglish (US)
Pages (from-to)4475-4482
Number of pages8
JournalCancer Research
Volume56
Issue number19
StatePublished - Oct 1 1996

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Allelic Imbalance
Microsatellite Instability
Adenocarcinoma
Chromosomes, Human, Pair 16
Tumor Suppressor Genes
Chromosomes, Human, Pair 8
Prostatic Neoplasms
Chromosomes
Chromosomes, Human, Pair 20
Chromosomes, Human, Pair 18
Chromosomes, Human, Pair 14
Neoplasms
Chromosomes, Human, Pair 10
Chromosomes, Human, Pair 5
Chromosomes, Human, Pair 7
Tetraploidy
Loss of Heterozygosity
Aneuploidy
Microsatellite Repeats
Phenotype

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Allelic imbalance and microsatellite instability in prostatic adenocarcinoma. / Cunningham, Julie M; Shan, Ailin; Wick, Myra J; McDonnell, Shannon K.; Schaid, Daniel J; Tester, David J.; Qian, Junqi; Takahashi, Satoru; Jenkins, Robert Brian; Bostwick, David G.; Thibodeau, Stephen N.

In: Cancer Research, Vol. 56, No. 19, 01.10.1996, p. 4475-4482.

Research output: Contribution to journalArticle

Cunningham, JM, Shan, A, Wick, MJ, McDonnell, SK, Schaid, DJ, Tester, DJ, Qian, J, Takahashi, S, Jenkins, RB, Bostwick, DG & Thibodeau, SN 1996, 'Allelic imbalance and microsatellite instability in prostatic adenocarcinoma', Cancer Research, vol. 56, no. 19, pp. 4475-4482.
Cunningham, Julie M ; Shan, Ailin ; Wick, Myra J ; McDonnell, Shannon K. ; Schaid, Daniel J ; Tester, David J. ; Qian, Junqi ; Takahashi, Satoru ; Jenkins, Robert Brian ; Bostwick, David G. ; Thibodeau, Stephen N. / Allelic imbalance and microsatellite instability in prostatic adenocarcinoma. In: Cancer Research. 1996 ; Vol. 56, No. 19. pp. 4475-4482.
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AU - Tester, David J.

AU - Qian, Junqi

AU - Takahashi, Satoru

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AU - Bostwick, David G.

AU - Thibodeau, Stephen N

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