@article{0f3012cd5cbf46ae9c96373fa7f7218c,
title = "Alemtuzumab (CAMPATH 1H) does not kill chronic lymphocytic leukemia cells in serum free medium",
abstract = "The mechanism of action of alemtuzumab (CAMPATH 1H) in chronic lymphocytic leukemia (CLL) is uncertain. We tested the hypothesis that alemtuzumab alone can induce apoptosis in cultured CLL cells. Purified peripheral blood B-lymphocytes from CLL patients were treated in serum free medium (AIM-V). There was minimal spontaneous apoptosis in untreated cells. Alemtuzumab ligation did not alter the membrane distribution of CD52 in single cells but many cells formed transient, small, tightly adherent clusters. Alemtuzumab alone did not induce apoptosis. In contrast, alemtuzumab plus complement was rapidly cytotoxic. We conclude that alemtuzumab does not cause apoptosis in purified CLL B cells cultured in serum free medium.",
keywords = "2-fluoroadenine-9-β-D- arabinofuranoside, ADCC, Antibody dependent cellular cytotoxicity, CAVHS, CLB, CLL, Central Arkansas Veterans Heathcare System, Chlorambucil, Chronic lymphocytic leukemia, F-ara-A, FISH, Fluorescent in situ hybridization, PI",
author = "Zent, {Clive S.} and Chen, {James B.} and Kurten, {Richard C.} and Kaushal, {Gur P.} and Lacy, {H. Marie} and Schichman, {Steven A.}",
note = "Funding Information: This work was funded in part by the Central Arkansas Veterans Healthcare System (CAVHS) Biomedical Research Foundation (CS Zent and SA Schichman); American Cancer Society Institutional Research Grant #IRG-021-06 (CS Zent and SA Schichman); ILEX Pharmaceuticals, L.P. (CS Zent); the Arkansas Science and Technology Authority (No. 01-B-11) (SA Schichman); the University of Arkansas for Medical Sciences (UAMS) Medical Research Endowment (SA Schichman); NIH grant #DK58324-01 awarded to GP Kaushal. The use of the facilities in the UAMS Digital and Confocal Microscopy Laboratory, supported by NIH Grants 1 P20 RR 16460, PAR-98-092, and 1-R24 CA82899, is acknowledged. Karen Marshall performed diagnostic flow cytometry and analysis of cell purification in the CAVHS clinical laboratory. Flow cytometric analysis for apoptosis was performed by Ashley Whitlow in the Department of Microbiology and Immunology Flow Cytometer Core Facility at the UAMS, under the direction of Kathleen Gilbert. LDH levels were done by the CAVHS clinical laboratory service under the direction of Debby Meece. Fab production and purification were performed with the generous assistance of Melinda Gunnell in the laboratory of Michael Owens in the Department of Pharmacology and Toxicology at the UAMS. The authors wish to thank Paul Dotson, Sarah Thornton, and Sharon Dobbins for assisting in collection of patient specimens, William Plunkett for advice on use of F-ara-A, Geoff Hale for advice on the use of alemtuzumab and cross-linking antibodies, Robert Safirstein for advice on apoptosis analysis, Rudolph Parrish for statistical advice, and Neil Kay for guidance in writing the manuscript. ",
year = "2004",
month = may,
doi = "10.1016/j.leukres.2003.09.011",
language = "English (US)",
volume = "28",
pages = "495--507",
journal = "Leukemia Research",
issn = "0145-2126",
publisher = "Elsevier Limited",
number = "5",
}