Alcohol consumption and breast tumor gene expression

Jun Wang, Yujing J. Heng, A. Heather Eliassen, Rulla M. Tamimi, Aditi Hazra, Vincent J. Carey, Christine B. Ambrosone, Victor P. de Andrade, Adam Brufsky, Fergus J Couch, Tari A. King, Francesmary Modugno, Celine M Vachon, David J. Hunter, Andrew H. Beck, Susan E. Hankinson

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Background: Alcohol consumption is an established risk factor for breast cancer and the association generally appears stronger among estrogen receptor (ER)-positive tumors. However, the biological mechanisms underlying this association are not completely understood. Methods: We analyzed messenger RNA (mRNA) microarray data from both invasive breast tumors (N=602) and tumor-adjacent normal tissues (N=508) from participants diagnosed with breast cancer in the Nurses' Health Study (NHS) and NHSII. Multivariable linear regression, controlling for other known breast cancer risk factors, was used to identify differentially expressed genes by pre-diagnostic alcohol intake. For pathway analysis, we performed gene set enrichment analysis (GSEA). Differentially expressed genes or enriched pathway-defined gene sets with false discovery rate (FDR) <0.1 identified in tumors were validated in RNA sequencing data of invasive breast tumors (N=166) from The Cancer Genome Atlas. Results: No individual genes were significantly differentially expressed by alcohol consumption in the NHS/NHSII. However, GSEA identified 33 and 68 pathway-defined gene sets at FDR <0.1 among 471 ER+ and 127 ER- tumors, respectively, all of which were validated. Among ER+ tumors, consuming 10+ grams of alcohol per day (vs. 0) was associated with upregulation in RNA metabolism and transport, cell cycle regulation, and DNA repair, and downregulation in lipid metabolism. Among ER- tumors, in addition to upregulation in RNA processing and cell cycle, alcohol intake was linked to overexpression of genes involved in cytokine signaling, including interferon and transforming growth factor (TGF)-β signaling pathways, and translation and post-translational modifications. Lower lipid metabolism was observed in both ER+ tumors and ER+ tumor-adjacent normal samples. Most of the significantly enriched gene sets identified in ER- tumors showed a similar enrichment pattern among ER- tumor-adjacent normal tissues. Conclusions: Our data suggest that moderate alcohol consumption (i.e. 10+ grams/day, equivalent to one or more drinks/day) is associated with several specific and reproducible biological processes and pathways, which adds potential new insight into alcohol-related breast carcinogenesis.

Original languageEnglish (US)
Article number108
JournalBreast Cancer Research
Volume19
Issue number1
DOIs
StatePublished - Sep 12 2017

Fingerprint

Alcohol Drinking
Estrogen Receptors
Breast Neoplasms
Gene Expression
Neoplasms
Genes
Alcohols
Lipid Metabolism
Cell Cycle
Up-Regulation
RNA Transport
Nurses
RNA Sequence Analysis
Biological Phenomena
Atlases
Health
Transforming Growth Factors
Post Translational Protein Processing
DNA Repair
Interferons

Keywords

  • Alcohol
  • Breast tumor
  • Epidemiology
  • Gene expression
  • Prospective

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

Cite this

Wang, J., Heng, Y. J., Eliassen, A. H., Tamimi, R. M., Hazra, A., Carey, V. J., ... Hankinson, S. E. (2017). Alcohol consumption and breast tumor gene expression. Breast Cancer Research, 19(1), [108]. https://doi.org/10.1186/s13058-017-0901-y

Alcohol consumption and breast tumor gene expression. / Wang, Jun; Heng, Yujing J.; Eliassen, A. Heather; Tamimi, Rulla M.; Hazra, Aditi; Carey, Vincent J.; Ambrosone, Christine B.; de Andrade, Victor P.; Brufsky, Adam; Couch, Fergus J; King, Tari A.; Modugno, Francesmary; Vachon, Celine M; Hunter, David J.; Beck, Andrew H.; Hankinson, Susan E.

In: Breast Cancer Research, Vol. 19, No. 1, 108, 12.09.2017.

Research output: Contribution to journalArticle

Wang, J, Heng, YJ, Eliassen, AH, Tamimi, RM, Hazra, A, Carey, VJ, Ambrosone, CB, de Andrade, VP, Brufsky, A, Couch, FJ, King, TA, Modugno, F, Vachon, CM, Hunter, DJ, Beck, AH & Hankinson, SE 2017, 'Alcohol consumption and breast tumor gene expression', Breast Cancer Research, vol. 19, no. 1, 108. https://doi.org/10.1186/s13058-017-0901-y
Wang J, Heng YJ, Eliassen AH, Tamimi RM, Hazra A, Carey VJ et al. Alcohol consumption and breast tumor gene expression. Breast Cancer Research. 2017 Sep 12;19(1). 108. https://doi.org/10.1186/s13058-017-0901-y
Wang, Jun ; Heng, Yujing J. ; Eliassen, A. Heather ; Tamimi, Rulla M. ; Hazra, Aditi ; Carey, Vincent J. ; Ambrosone, Christine B. ; de Andrade, Victor P. ; Brufsky, Adam ; Couch, Fergus J ; King, Tari A. ; Modugno, Francesmary ; Vachon, Celine M ; Hunter, David J. ; Beck, Andrew H. ; Hankinson, Susan E. / Alcohol consumption and breast tumor gene expression. In: Breast Cancer Research. 2017 ; Vol. 19, No. 1.
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AU - Eliassen, A. Heather

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AU - Carey, Vincent J.

AU - Ambrosone, Christine B.

AU - de Andrade, Victor P.

AU - Brufsky, Adam

AU - Couch, Fergus J

AU - King, Tari A.

AU - Modugno, Francesmary

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N2 - Background: Alcohol consumption is an established risk factor for breast cancer and the association generally appears stronger among estrogen receptor (ER)-positive tumors. However, the biological mechanisms underlying this association are not completely understood. Methods: We analyzed messenger RNA (mRNA) microarray data from both invasive breast tumors (N=602) and tumor-adjacent normal tissues (N=508) from participants diagnosed with breast cancer in the Nurses' Health Study (NHS) and NHSII. Multivariable linear regression, controlling for other known breast cancer risk factors, was used to identify differentially expressed genes by pre-diagnostic alcohol intake. For pathway analysis, we performed gene set enrichment analysis (GSEA). Differentially expressed genes or enriched pathway-defined gene sets with false discovery rate (FDR) <0.1 identified in tumors were validated in RNA sequencing data of invasive breast tumors (N=166) from The Cancer Genome Atlas. Results: No individual genes were significantly differentially expressed by alcohol consumption in the NHS/NHSII. However, GSEA identified 33 and 68 pathway-defined gene sets at FDR <0.1 among 471 ER+ and 127 ER- tumors, respectively, all of which were validated. Among ER+ tumors, consuming 10+ grams of alcohol per day (vs. 0) was associated with upregulation in RNA metabolism and transport, cell cycle regulation, and DNA repair, and downregulation in lipid metabolism. Among ER- tumors, in addition to upregulation in RNA processing and cell cycle, alcohol intake was linked to overexpression of genes involved in cytokine signaling, including interferon and transforming growth factor (TGF)-β signaling pathways, and translation and post-translational modifications. Lower lipid metabolism was observed in both ER+ tumors and ER+ tumor-adjacent normal samples. Most of the significantly enriched gene sets identified in ER- tumors showed a similar enrichment pattern among ER- tumor-adjacent normal tissues. Conclusions: Our data suggest that moderate alcohol consumption (i.e. 10+ grams/day, equivalent to one or more drinks/day) is associated with several specific and reproducible biological processes and pathways, which adds potential new insight into alcohol-related breast carcinogenesis.

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