Albumin In Situ Hybridization Can Be Positive in Adenocarcinomas and Other Tumors From Diverse Sites

Aqsa Nasir, Heidi D. Lehrke, Taofic Mounajjed, Samar Said, Lizhi Zhang, Saba Yasir, Sejal M Shah, Vishal S. Chandan, Thomas Christopher Smyrk, Roger K. Moreira, Jennifer M. Boland Froemming, Loren P. Herrera Hernandez, Tsung-Teh Wu, Rondell Graham

Research output: Contribution to journalArticle

Abstract

OBJECTIVES: Albumin messenger RNA (mRNA) expression is a marker of hepatocellular differentiation. Most published data are from review of tissue microarrays, and albumin in situ hybridization (ISH) expression across several tumor types is incompletely characterized. METHODS: Sections from 221 tumors were evaluated for albumin mRNA. Immunohistochemistry was used to confirm diagnoses. Albumin ISH was performed according to manufacturer-provided instructions. Fifty-nine cases were evaluated with both commercial ISH assays. RESULTS: Albumin mRNA was detected in all hepatocellular carcinomas (HCCs) and 81% of intrahepatic cholangiocarcinomas. Lung (20%), gallbladder (39%), hepatoid pancreatic (n = 1 of 1) adenocarcinoma, breast invasive ductal carcinoma (18%), yolk sac tumor (25%), and acinar cell carcinoma (29%) showed expression. Both assays were concordant in 93% of cases. CONCLUSIONS: Albumin ISH was expressed in all HCCs studied. It was also positive in intrahepatic cholangiocarcinoma and patchy positive in gallbladder adenocarcinoma and a subset of other neoplasms, which can be a potential pitfall.

Original languageEnglish (US)
Pages (from-to)190-199
Number of pages10
JournalAmerican journal of clinical pathology
Volume152
Issue number2
DOIs
StatePublished - Jul 5 2019

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In Situ Hybridization
Albumins
Adenocarcinoma
Neoplasms
Cholangiocarcinoma
Gallbladder
Messenger RNA
Hepatocellular Carcinoma
Acinar Cell Carcinoma
Endodermal Sinus Tumor
Carcinoma, Ductal, Breast
Differentiation Antigens
Immunohistochemistry
Lung

Keywords

  • Albumin
  • Hepatocellular
  • Hepatocellular carcinoma
  • In situ hybridization
  • Intrahepatic cholangiocarcinoma
  • Liver

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

Albumin In Situ Hybridization Can Be Positive in Adenocarcinomas and Other Tumors From Diverse Sites. / Nasir, Aqsa; Lehrke, Heidi D.; Mounajjed, Taofic; Said, Samar; Zhang, Lizhi; Yasir, Saba; Shah, Sejal M; Chandan, Vishal S.; Smyrk, Thomas Christopher; Moreira, Roger K.; Boland Froemming, Jennifer M.; Herrera Hernandez, Loren P.; Wu, Tsung-Teh; Graham, Rondell.

In: American journal of clinical pathology, Vol. 152, No. 2, 05.07.2019, p. 190-199.

Research output: Contribution to journalArticle

Nasir, A, Lehrke, HD, Mounajjed, T, Said, S, Zhang, L, Yasir, S, Shah, SM, Chandan, VS, Smyrk, TC, Moreira, RK, Boland Froemming, JM, Herrera Hernandez, LP, Wu, T-T & Graham, R 2019, 'Albumin In Situ Hybridization Can Be Positive in Adenocarcinomas and Other Tumors From Diverse Sites', American journal of clinical pathology, vol. 152, no. 2, pp. 190-199. https://doi.org/10.1093/ajcp/aqz032
Nasir, Aqsa ; Lehrke, Heidi D. ; Mounajjed, Taofic ; Said, Samar ; Zhang, Lizhi ; Yasir, Saba ; Shah, Sejal M ; Chandan, Vishal S. ; Smyrk, Thomas Christopher ; Moreira, Roger K. ; Boland Froemming, Jennifer M. ; Herrera Hernandez, Loren P. ; Wu, Tsung-Teh ; Graham, Rondell. / Albumin In Situ Hybridization Can Be Positive in Adenocarcinomas and Other Tumors From Diverse Sites. In: American journal of clinical pathology. 2019 ; Vol. 152, No. 2. pp. 190-199.
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abstract = "OBJECTIVES: Albumin messenger RNA (mRNA) expression is a marker of hepatocellular differentiation. Most published data are from review of tissue microarrays, and albumin in situ hybridization (ISH) expression across several tumor types is incompletely characterized. METHODS: Sections from 221 tumors were evaluated for albumin mRNA. Immunohistochemistry was used to confirm diagnoses. Albumin ISH was performed according to manufacturer-provided instructions. Fifty-nine cases were evaluated with both commercial ISH assays. RESULTS: Albumin mRNA was detected in all hepatocellular carcinomas (HCCs) and 81{\%} of intrahepatic cholangiocarcinomas. Lung (20{\%}), gallbladder (39{\%}), hepatoid pancreatic (n = 1 of 1) adenocarcinoma, breast invasive ductal carcinoma (18{\%}), yolk sac tumor (25{\%}), and acinar cell carcinoma (29{\%}) showed expression. Both assays were concordant in 93{\%} of cases. CONCLUSIONS: Albumin ISH was expressed in all HCCs studied. It was also positive in intrahepatic cholangiocarcinoma and patchy positive in gallbladder adenocarcinoma and a subset of other neoplasms, which can be a potential pitfall.",
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