Albumin In Situ Hybridization Can Be Positive in Adenocarcinomas and Other Tumors From Diverse Sites

Aqsa Nasir, Heidi D. Lehrke, Taofic Mounajjed, Samar Said, Lizhi Zhang, Saba Yasir, Sejal M Shah, Vishal S Chandran, Thomas Christopher Smyrk, Roger K. Moreira, Jennifer M. Boland Froemming, Loren P. Herrera Hernandez, Tsung Teh Wu, Rondell P. Graham

Research output: Contribution to journalArticlepeer-review

7 Scopus citations


OBJECTIVES: Albumin messenger RNA (mRNA) expression is a marker of hepatocellular differentiation. Most published data are from review of tissue microarrays, and albumin in situ hybridization (ISH) expression across several tumor types is incompletely characterized. METHODS: Sections from 221 tumors were evaluated for albumin mRNA. Immunohistochemistry was used to confirm diagnoses. Albumin ISH was performed according to manufacturer-provided instructions. Fifty-nine cases were evaluated with both commercial ISH assays. RESULTS: Albumin mRNA was detected in all hepatocellular carcinomas (HCCs) and 81% of intrahepatic cholangiocarcinomas. Lung (20%), gallbladder (39%), hepatoid pancreatic (n = 1 of 1) adenocarcinoma, breast invasive ductal carcinoma (18%), yolk sac tumor (25%), and acinar cell carcinoma (29%) showed expression. Both assays were concordant in 93% of cases. CONCLUSIONS: Albumin ISH was expressed in all HCCs studied. It was also positive in intrahepatic cholangiocarcinoma and patchy positive in gallbladder adenocarcinoma and a subset of other neoplasms, which can be a potential pitfall.

Original languageEnglish (US)
Pages (from-to)190-199
Number of pages10
JournalAmerican journal of clinical pathology
Issue number2
StatePublished - Jul 5 2019


  • Albumin
  • Hepatocellular
  • Hepatocellular carcinoma
  • In situ hybridization
  • Intrahepatic cholangiocarcinoma
  • Liver

ASJC Scopus subject areas

  • Pathology and Forensic Medicine


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