Airway epithelial cells respond to mechanical forces applied via collagen and RGD coated magnetic beads

D. Tschumperlin, M. Swartz, N. Wang, J. Drazen, J. Fredberg

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Airway epithelial cells, which synthesize and secrete inflammatory and fibrotic mediators, are exposed to mechanical stresses during bronchoconstriction. Because the deformations of the airway wall are transmitted to the airway epithelial cells via cell-cell and cell-matrix attachments, we investigated whether protein production is regulated by stresses applied directly to cell adhesion molecules. Mechanical stresses were applied to RGD and collagen coated beads bound to normal human bronchial epithelial cells using an adaptation of a previously described magnetic twisting device (1). Continuous stress resulted in increased Egr-1 protein at 30 minutes relative to cells with no beads, and cells with beads that were not stressed. Exposure of cells to both collagen and RGD coated beads decreased fibronectin protein at 24 hours, an effect that was partially (collagen) or totally (RGD) negated by 4 hours of continuous exposure to mechanical force. These preliminary results indicate the important role that the extracellular matrix, and forces transmitted via the extracellular matrix, may play in protein production in the normal and asthmatic airway.

Original languageEnglish (US)
Title of host publicationAnnual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings
PublisherIEEE
Number of pages1
ISBN (Print)0780356756
StatePublished - Dec 1 1999
EventProceedings of the 1999 IEEE Engineering in Medicine and Biology 21st Annual Conference and the 1999 Fall Meeting of the Biomedical Engineering Society (1st Joint BMES / EMBS) - Atlanta, GA, USA
Duration: Oct 13 1999Oct 16 1999

Publication series

NameAnnual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings
Volume1
ISSN (Print)0589-1019

Other

OtherProceedings of the 1999 IEEE Engineering in Medicine and Biology 21st Annual Conference and the 1999 Fall Meeting of the Biomedical Engineering Society (1st Joint BMES / EMBS)
CityAtlanta, GA, USA
Period10/13/9910/16/99

ASJC Scopus subject areas

  • Signal Processing
  • Biomedical Engineering
  • Computer Vision and Pattern Recognition
  • Health Informatics

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  • Cite this

    Tschumperlin, D., Swartz, M., Wang, N., Drazen, J., & Fredberg, J. (1999). Airway epithelial cells respond to mechanical forces applied via collagen and RGD coated magnetic beads. In Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings (Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings; Vol. 1). IEEE.