AIDS-associated progressive multifocal leukoencephalopathy (PML): Comparison to non-AIDS PML with in situ hybridization and immunohistochemistry

Allen Jr. Aksamit, Howard E. Gendelman, Jan M. Orenstein, Gholam H. Pezeshkpour

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Abstract

We studied brain sections from 10 patients with the acquired immunodeficiency syndrome (AIDS) and progressive multifocal leukoencephalopathy (PML) by in situ hybridization with a biotin-labeled JC virus (JCV) DNA probe and by immunohistochemistry using antibody against the JCV capsid antigen. We compared the results with brain sections studied in the same fashion from 10 PML patients without AIDS. The pathology of JCV infection in AIDS was similar to non-AIDS PML except for minor differences in degree. AIDS-associated pathologic material showed a greater tendency toward necrosis and a higher density of JCV-infected cells. Replication of JCV was restricted to glial cells in all tissue studied. Bizarre astrocytes were less frequent in the AIDS patients, and perivascular inflammatory cells were more frequent. We could not demonstrate JCV in macrophages or microglial cells known to harbor HIV infection. In situ hyridization with nonradioactive probes serves as a useful technique for the confirmation of PML in AIDS.

Original languageEnglish (US)
Pages (from-to)1073-1078
Number of pages6
JournalNeurology
Volume40
Issue number7
StatePublished - Jan 1 1990

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JC Virus
Progressive Multifocal Leukoencephalopathy
In Situ Hybridization
Acquired Immunodeficiency Syndrome
Immunohistochemistry
Capsid
DNA Probes
Brain
Virus Diseases
Biotin
Neuroglia
Astrocytes
HIV Infections
Necrosis
Macrophages
Pathology
Antigens
Antibodies

ASJC Scopus subject areas

  • Clinical Neurology

Cite this

AIDS-associated progressive multifocal leukoencephalopathy (PML) : Comparison to non-AIDS PML with in situ hybridization and immunohistochemistry. / Aksamit, Allen Jr.; Gendelman, Howard E.; Orenstein, Jan M.; Pezeshkpour, Gholam H.

In: Neurology, Vol. 40, No. 7, 01.01.1990, p. 1073-1078.

Research output: Contribution to journalArticle

Aksamit, Allen Jr. ; Gendelman, Howard E. ; Orenstein, Jan M. ; Pezeshkpour, Gholam H. / AIDS-associated progressive multifocal leukoencephalopathy (PML) : Comparison to non-AIDS PML with in situ hybridization and immunohistochemistry. In: Neurology. 1990 ; Vol. 40, No. 7. pp. 1073-1078.
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