Agreement of anti-neutrophil cytoplasmic antibody measurements obtained from serum and plasma

A. S. Lee, J. D. Finkielman, Tobias D Peikert, A. M. Hummel, M. A. Viss, G. L. Jacob, H. A. Homburger, Ulrich Specks

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Serum and plasma are used interchangeably to measure anti-neutrophil cytoplasmic antibodies (ANCA), even though the release of ANCA target antigens during the preparation of serum could affect ANCA assays and cause discrepancies between the results obtained from serum and plasma. To what extent ANCA test results obtained from serum agree and correlate with results from plasma remains unknown. Therefore, a comprehensive comparison was performed using serum and plasma samples which were collected in 175 patients with active Wegener's granulomatosis at enrolment of a recent randomized trial. These paired serum and plasma samples were subjected to parallel ANCA testing by standard indirect immunofluoresence on ethanol-fixed neutrophils, a direct enzyme-linked immunoassay (ELISA) for proteinase 3 (PR3)-ANCA and myeloperoxidase (MPO)-ANCA, and two different capture ELISAs for PR3-ANCA. The concordance of categorical serum and plasma ANCA results was assessed using κ-coefficients. These were > 0.8 for all assays, indicating a very good concordance between positive and negative serum and plasma results. Spearman's correlation coefficients for serum and plasma PR3-ANCA values obtained by direct ELISA and both capture ELISAs were ≥ 0.95 (P < 0.0001). Our study shows that serum and plasma samples can be used interchangeably for measuring ANCA.

Original languageEnglish (US)
Pages (from-to)15-20
Number of pages6
JournalClinical and Experimental Immunology
Volume146
Issue number1
DOIs
StatePublished - Oct 2006

Fingerprint

Antineutrophil Cytoplasmic Antibodies
Serum
Myeloblastin
Immunoenzyme Techniques
Enzyme-Linked Immunosorbent Assay
Granulomatosis with Polyangiitis
Peroxidase
Neutrophils
Ethanol

Keywords

  • Anti-neutrophil cytoplasmic
  • Antibodies
  • Enzyme-linked immunosorbent assay
  • Fluorescent antibody technique
  • Plasma
  • Serum

ASJC Scopus subject areas

  • Immunology

Cite this

Agreement of anti-neutrophil cytoplasmic antibody measurements obtained from serum and plasma. / Lee, A. S.; Finkielman, J. D.; Peikert, Tobias D; Hummel, A. M.; Viss, M. A.; Jacob, G. L.; Homburger, H. A.; Specks, Ulrich.

In: Clinical and Experimental Immunology, Vol. 146, No. 1, 10.2006, p. 15-20.

Research output: Contribution to journalArticle

Lee, A. S. ; Finkielman, J. D. ; Peikert, Tobias D ; Hummel, A. M. ; Viss, M. A. ; Jacob, G. L. ; Homburger, H. A. ; Specks, Ulrich. / Agreement of anti-neutrophil cytoplasmic antibody measurements obtained from serum and plasma. In: Clinical and Experimental Immunology. 2006 ; Vol. 146, No. 1. pp. 15-20.
@article{ce570e0b076c4518a03a8132a7af635e,
title = "Agreement of anti-neutrophil cytoplasmic antibody measurements obtained from serum and plasma",
abstract = "Serum and plasma are used interchangeably to measure anti-neutrophil cytoplasmic antibodies (ANCA), even though the release of ANCA target antigens during the preparation of serum could affect ANCA assays and cause discrepancies between the results obtained from serum and plasma. To what extent ANCA test results obtained from serum agree and correlate with results from plasma remains unknown. Therefore, a comprehensive comparison was performed using serum and plasma samples which were collected in 175 patients with active Wegener's granulomatosis at enrolment of a recent randomized trial. These paired serum and plasma samples were subjected to parallel ANCA testing by standard indirect immunofluoresence on ethanol-fixed neutrophils, a direct enzyme-linked immunoassay (ELISA) for proteinase 3 (PR3)-ANCA and myeloperoxidase (MPO)-ANCA, and two different capture ELISAs for PR3-ANCA. The concordance of categorical serum and plasma ANCA results was assessed using κ-coefficients. These were > 0.8 for all assays, indicating a very good concordance between positive and negative serum and plasma results. Spearman's correlation coefficients for serum and plasma PR3-ANCA values obtained by direct ELISA and both capture ELISAs were ≥ 0.95 (P < 0.0001). Our study shows that serum and plasma samples can be used interchangeably for measuring ANCA.",
keywords = "Anti-neutrophil cytoplasmic, Antibodies, Enzyme-linked immunosorbent assay, Fluorescent antibody technique, Plasma, Serum",
author = "Lee, {A. S.} and Finkielman, {J. D.} and Peikert, {Tobias D} and Hummel, {A. M.} and Viss, {M. A.} and Jacob, {G. L.} and Homburger, {H. A.} and Ulrich Specks",
year = "2006",
month = "10",
doi = "10.1111/j.1365-2249.2006.03179.x",
language = "English (US)",
volume = "146",
pages = "15--20",
journal = "Clinical and Experimental Immunology",
issn = "0009-9104",
publisher = "Wiley-Blackwell",
number = "1",

}

TY - JOUR

T1 - Agreement of anti-neutrophil cytoplasmic antibody measurements obtained from serum and plasma

AU - Lee, A. S.

AU - Finkielman, J. D.

AU - Peikert, Tobias D

AU - Hummel, A. M.

AU - Viss, M. A.

AU - Jacob, G. L.

AU - Homburger, H. A.

AU - Specks, Ulrich

PY - 2006/10

Y1 - 2006/10

N2 - Serum and plasma are used interchangeably to measure anti-neutrophil cytoplasmic antibodies (ANCA), even though the release of ANCA target antigens during the preparation of serum could affect ANCA assays and cause discrepancies between the results obtained from serum and plasma. To what extent ANCA test results obtained from serum agree and correlate with results from plasma remains unknown. Therefore, a comprehensive comparison was performed using serum and plasma samples which were collected in 175 patients with active Wegener's granulomatosis at enrolment of a recent randomized trial. These paired serum and plasma samples were subjected to parallel ANCA testing by standard indirect immunofluoresence on ethanol-fixed neutrophils, a direct enzyme-linked immunoassay (ELISA) for proteinase 3 (PR3)-ANCA and myeloperoxidase (MPO)-ANCA, and two different capture ELISAs for PR3-ANCA. The concordance of categorical serum and plasma ANCA results was assessed using κ-coefficients. These were > 0.8 for all assays, indicating a very good concordance between positive and negative serum and plasma results. Spearman's correlation coefficients for serum and plasma PR3-ANCA values obtained by direct ELISA and both capture ELISAs were ≥ 0.95 (P < 0.0001). Our study shows that serum and plasma samples can be used interchangeably for measuring ANCA.

AB - Serum and plasma are used interchangeably to measure anti-neutrophil cytoplasmic antibodies (ANCA), even though the release of ANCA target antigens during the preparation of serum could affect ANCA assays and cause discrepancies between the results obtained from serum and plasma. To what extent ANCA test results obtained from serum agree and correlate with results from plasma remains unknown. Therefore, a comprehensive comparison was performed using serum and plasma samples which were collected in 175 patients with active Wegener's granulomatosis at enrolment of a recent randomized trial. These paired serum and plasma samples were subjected to parallel ANCA testing by standard indirect immunofluoresence on ethanol-fixed neutrophils, a direct enzyme-linked immunoassay (ELISA) for proteinase 3 (PR3)-ANCA and myeloperoxidase (MPO)-ANCA, and two different capture ELISAs for PR3-ANCA. The concordance of categorical serum and plasma ANCA results was assessed using κ-coefficients. These were > 0.8 for all assays, indicating a very good concordance between positive and negative serum and plasma results. Spearman's correlation coefficients for serum and plasma PR3-ANCA values obtained by direct ELISA and both capture ELISAs were ≥ 0.95 (P < 0.0001). Our study shows that serum and plasma samples can be used interchangeably for measuring ANCA.

KW - Anti-neutrophil cytoplasmic

KW - Antibodies

KW - Enzyme-linked immunosorbent assay

KW - Fluorescent antibody technique

KW - Plasma

KW - Serum

UR - http://www.scopus.com/inward/record.url?scp=33748701733&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33748701733&partnerID=8YFLogxK

U2 - 10.1111/j.1365-2249.2006.03179.x

DO - 10.1111/j.1365-2249.2006.03179.x

M3 - Article

C2 - 16968393

AN - SCOPUS:33748701733

VL - 146

SP - 15

EP - 20

JO - Clinical and Experimental Immunology

JF - Clinical and Experimental Immunology

SN - 0009-9104

IS - 1

ER -