Agonists of proteinase-activated receptor 2 excite guinea pig ileal myenteric neurons

David R. Linden, Brian P. Manning, Nigel W. Bunnett, Gary M. Mawe

Research output: Contribution to journalArticle

39 Scopus citations

Abstract

The effects of proteinase-activated receptor 2 (PAR2) agonists on the electrical properties of intact guinea pig ileal myenteric neurons were measured with intracellular microelectrodes. Approximately 52% of AH neurons and 41% of S neurons responded to pressure ejection of SLIGRL-NH2 or trypsin with a prolonged depolarization that was often accompanied by increased excitability. When added to the bathing solution, trypsin caused a concentration-dependent depolarization of responding neurons with an estimated EC50 value of 87 nM. Collectively, these novel observations indicate that PAR2 excites a proportion of myenteric neurons, which may contribute to dysmotility during intestinal inflammation.

Original languageEnglish (US)
Pages (from-to)311-314
Number of pages4
JournalEuropean Journal of Pharmacology
Volume431
Issue number3
DOIs
StatePublished - Nov 23 2001

Keywords

  • Electrophysiology
  • Euteric nervous system
  • Neuroimmune interaction
  • Thrombin receptor
  • Trypsin

ASJC Scopus subject areas

  • Pharmacology

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