Agonists block currents through acetylcholine receptor channels

S. M. Sine; J. H. Steinbach

doi:10.1016/S0006-3495(84)84022-9

Agonists block currents through acetylcholine receptor channels

S. M. Sine, J. H. Steinbach

Physiology & Biomedical Engineering

Research output: Contribution to journal › Article › peer-review

117 Scopus citations

Abstract

We have examined the effects of high concentrations of cholinergic agonists on currents through single acetylcholine receptor (AChR) channels on clonal BC3H1 cells. We find that raised concentrations of acetylcholine (ACh; above 300 microM) or carbamylcholine (Carb; above 1,000 microM) produce a voltage- and concentration-dependent reduction in the mean single-channel current. Raised concentrations of suberyldicholine (Sub; above 3 microM) produce a voltage- and concentration-dependent increase in the number of brief duration low-conductance interruptions of open-channel currents. These observations can be quantitatively described by a model in which agonist molecules enter and transiently occlude the ion-channel of the AChR.

Original language	English (US)
Pages (from-to)	277-283
Number of pages	7
Journal	Biophysical Journal
Volume	46
Issue number	2
DOIs	https://doi.org/10.1016/S0006-3495(84)84022-9
State	Published - 1984

ASJC Scopus subject areas

Biophysics

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10.1016/S0006-3495(84)84022-9

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title = "Agonists block currents through acetylcholine receptor channels",

abstract = "We have examined the effects of high concentrations of cholinergic agonists on currents through single acetylcholine receptor (AChR) channels on clonal BC3H1 cells. We find that raised concentrations of acetylcholine (ACh; above 300 microM) or carbamylcholine (Carb; above 1,000 microM) produce a voltage- and concentration-dependent reduction in the mean single-channel current. Raised concentrations of suberyldicholine (Sub; above 3 microM) produce a voltage- and concentration-dependent increase in the number of brief duration low-conductance interruptions of open-channel currents. These observations can be quantitatively described by a model in which agonist molecules enter and transiently occlude the ion-channel of the AChR.",

author = "Sine, {S. M.} and Steinbach, {J. H.}",

note = "Funding Information: Supported by grants BNS-79-0613 from the National Science Founda- tion, NS-13719 from the National Institutes of Health, and Office of Naval Research contract N-00014-79-C-0798 to J. H. Steinbach. S. M. Sine was supported by postdoctoral fellowships from the Muscular Dystrophy Association, then the National Institutes of Health. J. H. Steinbach is a Sloan Foundation Fellow.",

year = "1984",

doi = "10.1016/S0006-3495(84)84022-9",

language = "English (US)",

volume = "46",

pages = "277--283",

journal = "Biophysical Journal",

issn = "0006-3495",

publisher = "Biophysical Society",

number = "2",

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TY - JOUR

T1 - Agonists block currents through acetylcholine receptor channels

AU - Sine, S. M.

AU - Steinbach, J. H.

N1 - Funding Information: Supported by grants BNS-79-0613 from the National Science Founda- tion, NS-13719 from the National Institutes of Health, and Office of Naval Research contract N-00014-79-C-0798 to J. H. Steinbach. S. M. Sine was supported by postdoctoral fellowships from the Muscular Dystrophy Association, then the National Institutes of Health. J. H. Steinbach is a Sloan Foundation Fellow.

PY - 1984

Y1 - 1984

N2 - We have examined the effects of high concentrations of cholinergic agonists on currents through single acetylcholine receptor (AChR) channels on clonal BC3H1 cells. We find that raised concentrations of acetylcholine (ACh; above 300 microM) or carbamylcholine (Carb; above 1,000 microM) produce a voltage- and concentration-dependent reduction in the mean single-channel current. Raised concentrations of suberyldicholine (Sub; above 3 microM) produce a voltage- and concentration-dependent increase in the number of brief duration low-conductance interruptions of open-channel currents. These observations can be quantitatively described by a model in which agonist molecules enter and transiently occlude the ion-channel of the AChR.

AB - We have examined the effects of high concentrations of cholinergic agonists on currents through single acetylcholine receptor (AChR) channels on clonal BC3H1 cells. We find that raised concentrations of acetylcholine (ACh; above 300 microM) or carbamylcholine (Carb; above 1,000 microM) produce a voltage- and concentration-dependent reduction in the mean single-channel current. Raised concentrations of suberyldicholine (Sub; above 3 microM) produce a voltage- and concentration-dependent increase in the number of brief duration low-conductance interruptions of open-channel currents. These observations can be quantitatively described by a model in which agonist molecules enter and transiently occlude the ion-channel of the AChR.

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U2 - 10.1016/S0006-3495(84)84022-9

DO - 10.1016/S0006-3495(84)84022-9

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AN - SCOPUS:0021150988

SN - 0006-3495

VL - 46

SP - 277

EP - 283

JO - Biophysical Journal

JF - Biophysical Journal

IS - 2

ER -

Agonists block currents through acetylcholine receptor channels

Abstract

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