Agonist regulation of muscarinic acetylcholine receptors in rat spinal cord

J. E. Taylor, T. L. Yaksh, E. Richelson

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

In vitro studies with culture cells originating from nervous tissue have shown that chronic exposure to muscarinic agonists results in a loss of muscarinic receptors. To determine whether this type of regulation of muscarinic receptor number also occurs in vivo, we infused carbachol into the spinal cords of rats. A single carbachol injection into the lumbar spinal cord caused a significant increase in the nociceptive threshold. This effect of carbachol diminished to control levels after 12 h of repeated agonist injections every 4 h and was blocked by atropine. The desensitization to the antinociceptive effects of carbachol was associated with a loss of muscarinic receptors as determined by the binding of the muscarinic antagonist [3H]quinuclidinyl benzilate. After a 24-h exposure to carbachol given every 4 h, there was about a 60% loss of binding sites. The loss of muscarinic receptors was also blocked by atropine and was reversible. These results represent direct evidence that a muscarnic agonist can regulate receptor number in the central nervous system and suggest that this loss of receptors is associated with a desensitization to the antinociceptive effects of carbachol injected into the spinal cord.

Original languageEnglish (US)
Pages (from-to)521-524
Number of pages4
JournalJournal of Neurochemistry
Volume39
Issue number2
StatePublished - 1982

Fingerprint

Carbachol
Muscarinic Receptors
Rats
Spinal Cord
Atropine
Quinuclidinyl Benzilate
Nerve Tissue
Muscarinic Agonists
Injections
Muscarinic Antagonists
Level control
Neurology
Cell culture
Central Nervous System
Cell Culture Techniques
Binding Sites
Tissue

ASJC Scopus subject areas

  • Biochemistry
  • Cellular and Molecular Neuroscience

Cite this

Taylor, J. E., Yaksh, T. L., & Richelson, E. (1982). Agonist regulation of muscarinic acetylcholine receptors in rat spinal cord. Journal of Neurochemistry, 39(2), 521-524.

Agonist regulation of muscarinic acetylcholine receptors in rat spinal cord. / Taylor, J. E.; Yaksh, T. L.; Richelson, E.

In: Journal of Neurochemistry, Vol. 39, No. 2, 1982, p. 521-524.

Research output: Contribution to journalArticle

Taylor, JE, Yaksh, TL & Richelson, E 1982, 'Agonist regulation of muscarinic acetylcholine receptors in rat spinal cord', Journal of Neurochemistry, vol. 39, no. 2, pp. 521-524.
Taylor, J. E. ; Yaksh, T. L. ; Richelson, E. / Agonist regulation of muscarinic acetylcholine receptors in rat spinal cord. In: Journal of Neurochemistry. 1982 ; Vol. 39, No. 2. pp. 521-524.
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