Agonist modulation of intracellular calcium oscillations in tracheal smooth muscle cells

Gary C Sieck, Y.s. Prakash, M. S. Kannan

Research output: Contribution to journalArticle

Abstract

!n freshly dissociated porcine trachéal smooth muscle (TSM) cells loaded with fluo-3, the dynamics of intraceliular Ca2+ ([Ca2+]i) responses to ACh and endothelin-I (ET-I) were examined using a real-time confocal microscope. ACh (10 nM-IO μM) induced [Ca2+]i oscillations that did not vary in amplitude and persisted in the absence of extracellular Ca2+. Oscillation frequency, but not amplitude, depended on ACh concentration, increasing - 3 fold across the ACh range. Oscillation frequency also slowed during the course of exposure to a given ACh concentration, suggesting muscarinic receptor desensitization. The rise (0.4 to 2 s) and fall (I to 4 s) times of each [Ca], wave did not vary with ACh concentration, but wave velocity increased. ET-1 ( 10 nM-1 u,M) also induced [Ca], oscillations of comparable amplitudes to ACh. However, unlike ACh, oscillation frequency and wave velocity were not dependent on ET-1 concentration. The relatively constant amplitude of [Ca2]i waves in response to varying concentrations of both ACh and ET-1 suggests an all-or-none event at the level of SR Cu4 release in TSM cells. It is likely that control of oscillation frequency and wave velocity are major factors in ACh regulation of [Ca '], but not ET-1. Variations in [Ca], exposure time could play an important role in regulating smooth muscle contractions or other Ca2-dependent processes. Supported by N!H grant HI,51736 and by fellowships from Abbott Laboratories.

Original languageEnglish (US)
JournalFASEB Journal
Volume10
Issue number3
StatePublished - 1996

Fingerprint

Calcium Signaling
smooth muscle
myocytes
agonists
Smooth Muscle Myocytes
Muscle
oscillation
Cells
Modulation
Calcium
calcium
Organized Financing
Endothelins
Muscarinic Receptors
Muscle Contraction
Smooth Muscle
Swine
endothelins
muscle contraction
Microscopes

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Cell Biology

Cite this

Agonist modulation of intracellular calcium oscillations in tracheal smooth muscle cells. / Sieck, Gary C; Prakash, Y.s.; Kannan, M. S.

In: FASEB Journal, Vol. 10, No. 3, 1996.

Research output: Contribution to journalArticle

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