Agonist-mediated conformational changes in acetylcholine-binding protein revealed by simulation and intrinsic tryptophan fluorescence

Fan Gao, Nina Bren, Thomas P Burghardt, Scott Hansen, Richard H. Henchman, Palmer Taylor, J. Andrew McCammon, Steven M Sine

Research output: Contribution to journalArticle

114 Citations (Scopus)

Abstract

We delineated acetylcholine (ACh)-dependent conformational changes in a prototype of the nicotinic receptor ligand binding domain by molecular dynamics simulation and changes in intrinsic tryptophan (Trp) fluorescence. Prolonged molecular dynamics simulation of ACh-binding protein showed that binding of ACh establishes close register of Trps from adjacent subunits, Trp143 and Trp53, and draws the peripheral C-loop inward to occlude the entrance to the binding cavity. Close register of Trp143 and Trp 53 was demonstrated by ACh-mediated quenching of intrinsic Trp fluorescence, elimination of quenching by mutation of one or both Trps to Phe, and decreased lifetime of Trp fluorescence by bound ACh. Occlusion of the binding cavity by the C-loop was demonstrated by restricted access of an extrinsic quencher of binding site Trp fluorescence by ACh. The collective findings showed that ACh initially establishes close register of conserved Trps from adjacent subunits and then draws the C-loop inward to occlude the entrance to the binding cavity.

Original languageEnglish (US)
Pages (from-to)8443-8451
Number of pages9
JournalJournal of Biological Chemistry
Volume280
Issue number9
DOIs
StatePublished - Mar 4 2005

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Tryptophan
Acetylcholine
Carrier Proteins
Fluorescence
Molecular Dynamics Simulation
Molecular dynamics
Quenching
Computer simulation
Nicotinic Receptors
Protein Binding
Binding Sites
Ligands
Mutation

ASJC Scopus subject areas

  • Biochemistry

Cite this

Agonist-mediated conformational changes in acetylcholine-binding protein revealed by simulation and intrinsic tryptophan fluorescence. / Gao, Fan; Bren, Nina; Burghardt, Thomas P; Hansen, Scott; Henchman, Richard H.; Taylor, Palmer; McCammon, J. Andrew; Sine, Steven M.

In: Journal of Biological Chemistry, Vol. 280, No. 9, 04.03.2005, p. 8443-8451.

Research output: Contribution to journalArticle

Gao, Fan ; Bren, Nina ; Burghardt, Thomas P ; Hansen, Scott ; Henchman, Richard H. ; Taylor, Palmer ; McCammon, J. Andrew ; Sine, Steven M. / Agonist-mediated conformational changes in acetylcholine-binding protein revealed by simulation and intrinsic tryptophan fluorescence. In: Journal of Biological Chemistry. 2005 ; Vol. 280, No. 9. pp. 8443-8451.
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AU - Bren, Nina

AU - Burghardt, Thomas P

AU - Hansen, Scott

AU - Henchman, Richard H.

AU - Taylor, Palmer

AU - McCammon, J. Andrew

AU - Sine, Steven M

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