Agonist-induced cyclic ADP ribose production in airway smooth muscle

Sertac N. Kip; Molly Smelter; Adeyemi Iyanoye; Eduardo N. Chini; Y. S. Prakash; Christina M. Pabelick; Gary C. Sieck

doi:10.1016/j.abb.2006.06.014

Agonist-induced cyclic ADP ribose production in airway smooth muscle

Sertac N. Kip, Molly Smelter, Adeyemi Iyanoye, Eduardo N. Chini, Y. S. Prakash, Christina M. Pabelick, Gary C. Sieck

Research output: Contribution to journal › Article › peer-review

13 Scopus citations

Abstract

Cyclic ADP-ribose (cADPR) triggers sarcoplasmic reticulum (SR) Ca²⁺ release in airway smooth muscle (ASM). SR Ca²⁺ release is an important component of the intracellular Ca²⁺ ([Ca²⁺]_i) response of ASM to agonists. Whether cADPR is endogenously produced in ASM during agonist stimulation has not been established. In this study, cADPR production was examined in acutely dissociated porcine ASM cells. ACh stimulation (≥1 μM) significantly increased cADPR levels, peaking between 30 s and 1 min. This effect was inhibited by M₂ and M₃ muscarinic receptor antagonists. Histamine (≥5 μM) increased cADPR levels to a greater extent than ACh, while diphenhydramine blocked histamine-induced cADPR elevation. Both bradykinin (100 nM) and endothelin-1 (100 nM) also increased cADPR levels to a greater extent than ACh or histamine. These results indicate that in porcine ASM, certain agonists acting via receptors increase cADPR levels. Furthermore, the extent of cADPR responses to agonist varies, possibly reflecting differences in G-protein coupling.

Original language	English (US)
Pages (from-to)	102-107
Number of pages	6
Journal	Archives of Biochemistry and Biophysics
Volume	452
Issue number	2
DOIs	https://doi.org/10.1016/j.abb.2006.06.014
State	Published - Aug 15 2006

Keywords

ADP ribosyl cyclase
Acetylcholine
Bradykinin
CD38
Endothelin
G-protein
Histamine
Muscarinic

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology

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10.1016/j.abb.2006.06.014

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@article{d827ae4ea90b4b2fa7fa09105f41f3f1,

title = "Agonist-induced cyclic ADP ribose production in airway smooth muscle",

abstract = "Cyclic ADP-ribose (cADPR) triggers sarcoplasmic reticulum (SR) Ca2+ release in airway smooth muscle (ASM). SR Ca2+ release is an important component of the intracellular Ca2+ ([Ca2+]i) response of ASM to agonists. Whether cADPR is endogenously produced in ASM during agonist stimulation has not been established. In this study, cADPR production was examined in acutely dissociated porcine ASM cells. ACh stimulation (≥1 μM) significantly increased cADPR levels, peaking between 30 s and 1 min. This effect was inhibited by M2 and M3 muscarinic receptor antagonists. Histamine (≥5 μM) increased cADPR levels to a greater extent than ACh, while diphenhydramine blocked histamine-induced cADPR elevation. Both bradykinin (100 nM) and endothelin-1 (100 nM) also increased cADPR levels to a greater extent than ACh or histamine. These results indicate that in porcine ASM, certain agonists acting via receptors increase cADPR levels. Furthermore, the extent of cADPR responses to agonist varies, possibly reflecting differences in G-protein coupling.",

keywords = "ADP ribosyl cyclase, Acetylcholine, Bradykinin, CD38, Endothelin, G-protein, Histamine, Muscarinic",

author = "Kip, {Sertac N.} and Molly Smelter and Adeyemi Iyanoye and Chini, {Eduardo N.} and Prakash, {Y. S.} and Pabelick, {Christina M.} and Sieck, {Gary C.}",

note = "Funding Information: The authors gratefully acknowledge the technical expertise of Mr. Michael Thompson and Mr. Larry Hunter. This work was supported by grants from the National Institutes of Health (NIH; HL74309 and GM56686; AI, EC, YSP, CMP, GCS), the Foundation for Anesthesia Education and Research (FAER; CMP), the American Heart Association (AHA; EC), and the Mayo Foundation.",

year = "2006",

month = aug,

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doi = "10.1016/j.abb.2006.06.014",

language = "English (US)",

volume = "452",

pages = "102--107",

journal = "Archives of Biochemistry and Biophysics",

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T1 - Agonist-induced cyclic ADP ribose production in airway smooth muscle

AU - Kip, Sertac N.

AU - Smelter, Molly

AU - Iyanoye, Adeyemi

AU - Chini, Eduardo N.

AU - Prakash, Y. S.

AU - Pabelick, Christina M.

AU - Sieck, Gary C.

N1 - Funding Information: The authors gratefully acknowledge the technical expertise of Mr. Michael Thompson and Mr. Larry Hunter. This work was supported by grants from the National Institutes of Health (NIH; HL74309 and GM56686; AI, EC, YSP, CMP, GCS), the Foundation for Anesthesia Education and Research (FAER; CMP), the American Heart Association (AHA; EC), and the Mayo Foundation.

PY - 2006/8/15

Y1 - 2006/8/15

N2 - Cyclic ADP-ribose (cADPR) triggers sarcoplasmic reticulum (SR) Ca2+ release in airway smooth muscle (ASM). SR Ca2+ release is an important component of the intracellular Ca2+ ([Ca2+]i) response of ASM to agonists. Whether cADPR is endogenously produced in ASM during agonist stimulation has not been established. In this study, cADPR production was examined in acutely dissociated porcine ASM cells. ACh stimulation (≥1 μM) significantly increased cADPR levels, peaking between 30 s and 1 min. This effect was inhibited by M2 and M3 muscarinic receptor antagonists. Histamine (≥5 μM) increased cADPR levels to a greater extent than ACh, while diphenhydramine blocked histamine-induced cADPR elevation. Both bradykinin (100 nM) and endothelin-1 (100 nM) also increased cADPR levels to a greater extent than ACh or histamine. These results indicate that in porcine ASM, certain agonists acting via receptors increase cADPR levels. Furthermore, the extent of cADPR responses to agonist varies, possibly reflecting differences in G-protein coupling.

AB - Cyclic ADP-ribose (cADPR) triggers sarcoplasmic reticulum (SR) Ca2+ release in airway smooth muscle (ASM). SR Ca2+ release is an important component of the intracellular Ca2+ ([Ca2+]i) response of ASM to agonists. Whether cADPR is endogenously produced in ASM during agonist stimulation has not been established. In this study, cADPR production was examined in acutely dissociated porcine ASM cells. ACh stimulation (≥1 μM) significantly increased cADPR levels, peaking between 30 s and 1 min. This effect was inhibited by M2 and M3 muscarinic receptor antagonists. Histamine (≥5 μM) increased cADPR levels to a greater extent than ACh, while diphenhydramine blocked histamine-induced cADPR elevation. Both bradykinin (100 nM) and endothelin-1 (100 nM) also increased cADPR levels to a greater extent than ACh or histamine. These results indicate that in porcine ASM, certain agonists acting via receptors increase cADPR levels. Furthermore, the extent of cADPR responses to agonist varies, possibly reflecting differences in G-protein coupling.

KW - ADP ribosyl cyclase

KW - Acetylcholine

KW - Bradykinin

KW - CD38

KW - Endothelin

KW - G-protein

KW - Histamine

KW - Muscarinic

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JO - Archives of Biochemistry and Biophysics

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IS - 2

ER -

Agonist-induced cyclic ADP ribose production in airway smooth muscle

Abstract

Keywords

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