Age-related changes in functional NANC innervation with VIP and substance P in the jejunum of Lewis rats

Michael S. Kasparek; Javairiah Fatima; Corey W. Iqbal; Judith A. Duenes; Michael G. Sarr

doi:10.1016/j.autneu.2009.08.004

Age-related changes in functional NANC innervation with VIP and substance P in the jejunum of Lewis rats

Michael S. Kasparek, Javairiah Fatima, Corey W. Iqbal, Judith A. Duenes, Michael G. Sarr

Gastroenterologic and General Surgery

Research output: Contribution to journal › Article › peer-review

8 Scopus citations

Abstract

Age-related changes in non-adrenergic, non-cholinergic (NANC) neurotransmission might contribute to differences in gastrointestinal motility. Our aim was to determine age-related changes in functional innervation with vasoactive intestinal polypeptide (VIP) and substance P (Sub P) in rat jejunum. We hypothesized that maturation causes changes in neurotransmission with these two neuropeptides. Longitudinal and circular jejunal muscle strips from young (3 months) and middle-aged (15 months) rats (total: 24 rats) were studied; the response to exogenous VIP and Sub P and the effect of their endogenous release from the enteric nervous system during electrical field stimulation (EFS) were evaluated. In longitudinal muscle, response to exogenous VIP and endogenously released VIP during EFS were increased in middle-aged rats, while the effect of endogenously released Sub P was decreased. In the circular muscle, the response to endogenously released VIP was increased in middle-aged rats, while the effects of exogenous VIP and endogenously released Sub P were unchanged. Response to exogenous Sub P was unaffected by maturation in both muscle layers. Spontaneous contractile activity was increased in the longitudinal and circular muscle of the older rats. In the jejunum of middle-aged rats, participation of VIP in functional NANC innervation was increased, while functional innervation with Sub P was decreased. These changes in the balance of inhibitory and excitatory neurotransmission occur during the year of maturation in rats and demonstrate an age-dependant plasticity of neuromuscular bowel function.

Original language	English (US)
Pages (from-to)	127-134
Number of pages	8
Journal	Autonomic Neuroscience: Basic and Clinical
Volume	151
Issue number	2
DOIs	https://doi.org/10.1016/j.autneu.2009.08.004
State	Published - Dec 3 2009

Keywords

Aging
Enteric nervous system
Maturation
Motility
Small intestine
Substance P
Vasoactive intestinal polypeptide

ASJC Scopus subject areas

Endocrine and Autonomic Systems
Clinical Neurology
Cellular and Molecular Neuroscience

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10.1016/j.autneu.2009.08.004

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title = "Age-related changes in functional NANC innervation with VIP and substance P in the jejunum of Lewis rats",

abstract = "Age-related changes in non-adrenergic, non-cholinergic (NANC) neurotransmission might contribute to differences in gastrointestinal motility. Our aim was to determine age-related changes in functional innervation with vasoactive intestinal polypeptide (VIP) and substance P (Sub P) in rat jejunum. We hypothesized that maturation causes changes in neurotransmission with these two neuropeptides. Longitudinal and circular jejunal muscle strips from young (3 months) and middle-aged (15 months) rats (total: 24 rats) were studied; the response to exogenous VIP and Sub P and the effect of their endogenous release from the enteric nervous system during electrical field stimulation (EFS) were evaluated. In longitudinal muscle, response to exogenous VIP and endogenously released VIP during EFS were increased in middle-aged rats, while the effect of endogenously released Sub P was decreased. In the circular muscle, the response to endogenously released VIP was increased in middle-aged rats, while the effects of exogenous VIP and endogenously released Sub P were unchanged. Response to exogenous Sub P was unaffected by maturation in both muscle layers. Spontaneous contractile activity was increased in the longitudinal and circular muscle of the older rats. In the jejunum of middle-aged rats, participation of VIP in functional NANC innervation was increased, while functional innervation with Sub P was decreased. These changes in the balance of inhibitory and excitatory neurotransmission occur during the year of maturation in rats and demonstrate an age-dependant plasticity of neuromuscular bowel function.",

keywords = "Aging, Enteric nervous system, Maturation, Motility, Small intestine, Substance P, Vasoactive intestinal polypeptide",

author = "Kasparek, {Michael S.} and Javairiah Fatima and Iqbal, {Corey W.} and Duenes, {Judith A.} and Sarr, {Michael G.}",

note = "Funding Information: Supported by grant DK 39337 from the National Institutes of Health, United States Public Health Services (M.G.S.) and grant KA 2329/1-1 from the Deutsche Forschungsgemeinschaft, Germany (M.S.K.) .",

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AU - Kasparek, Michael S.

AU - Fatima, Javairiah

AU - Iqbal, Corey W.

AU - Duenes, Judith A.

AU - Sarr, Michael G.

N1 - Funding Information: Supported by grant DK 39337 from the National Institutes of Health, United States Public Health Services (M.G.S.) and grant KA 2329/1-1 from the Deutsche Forschungsgemeinschaft, Germany (M.S.K.) .

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N2 - Age-related changes in non-adrenergic, non-cholinergic (NANC) neurotransmission might contribute to differences in gastrointestinal motility. Our aim was to determine age-related changes in functional innervation with vasoactive intestinal polypeptide (VIP) and substance P (Sub P) in rat jejunum. We hypothesized that maturation causes changes in neurotransmission with these two neuropeptides. Longitudinal and circular jejunal muscle strips from young (3 months) and middle-aged (15 months) rats (total: 24 rats) were studied; the response to exogenous VIP and Sub P and the effect of their endogenous release from the enteric nervous system during electrical field stimulation (EFS) were evaluated. In longitudinal muscle, response to exogenous VIP and endogenously released VIP during EFS were increased in middle-aged rats, while the effect of endogenously released Sub P was decreased. In the circular muscle, the response to endogenously released VIP was increased in middle-aged rats, while the effects of exogenous VIP and endogenously released Sub P were unchanged. Response to exogenous Sub P was unaffected by maturation in both muscle layers. Spontaneous contractile activity was increased in the longitudinal and circular muscle of the older rats. In the jejunum of middle-aged rats, participation of VIP in functional NANC innervation was increased, while functional innervation with Sub P was decreased. These changes in the balance of inhibitory and excitatory neurotransmission occur during the year of maturation in rats and demonstrate an age-dependant plasticity of neuromuscular bowel function.

AB - Age-related changes in non-adrenergic, non-cholinergic (NANC) neurotransmission might contribute to differences in gastrointestinal motility. Our aim was to determine age-related changes in functional innervation with vasoactive intestinal polypeptide (VIP) and substance P (Sub P) in rat jejunum. We hypothesized that maturation causes changes in neurotransmission with these two neuropeptides. Longitudinal and circular jejunal muscle strips from young (3 months) and middle-aged (15 months) rats (total: 24 rats) were studied; the response to exogenous VIP and Sub P and the effect of their endogenous release from the enteric nervous system during electrical field stimulation (EFS) were evaluated. In longitudinal muscle, response to exogenous VIP and endogenously released VIP during EFS were increased in middle-aged rats, while the effect of endogenously released Sub P was decreased. In the circular muscle, the response to endogenously released VIP was increased in middle-aged rats, while the effects of exogenous VIP and endogenously released Sub P were unchanged. Response to exogenous Sub P was unaffected by maturation in both muscle layers. Spontaneous contractile activity was increased in the longitudinal and circular muscle of the older rats. In the jejunum of middle-aged rats, participation of VIP in functional NANC innervation was increased, while functional innervation with Sub P was decreased. These changes in the balance of inhibitory and excitatory neurotransmission occur during the year of maturation in rats and demonstrate an age-dependant plasticity of neuromuscular bowel function.

KW - Aging

KW - Enteric nervous system

KW - Maturation

KW - Motility

KW - Small intestine

KW - Substance P

KW - Vasoactive intestinal polypeptide

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JO - Autonomic Neuroscience: Basic and Clinical

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ER -

Age-related changes in functional NANC innervation with VIP and substance P in the jejunum of Lewis rats

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Keywords

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