Affinity maturation of antibodies requires integrity of the adult thymus

Mouhammed Abuattieh, Diane Bender, Esther Liu, Peter Wettstein, Jeffrey L. Platt, Marilia Cascalho

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

The generation of B-cell responses to proteins requires a functional thymus to produce CD4 + T cells which helps in the activation and differentiation of B cells. Because the mature T-cell repertoire has abundant cells with the helper phenotype, one might predict that in mature individuals, the generation of B-cell memory would proceed independently of the thymus. Contrary to that prediction, we show here that the removal of the thymus after the establishment of the T-cell compartment or sham surgery without removal of the thymus impairs the affinity maturation of antibodies. Because removal or manipulation of the thymus did not decrease the frequency of mutation of the Ig variable heavy chain exons encoding antigen-specific antibodies, we conclude that the thymus controls affinity maturation of antibodies in the mature individual by facilitating the selection of B cells with high-affinity antibodies.

Original languageEnglish (US)
Pages (from-to)500-510
Number of pages11
JournalEuropean Journal of Immunology
Volume42
Issue number2
DOIs
StatePublished - Feb 2012

Fingerprint

Antibody Affinity
Thymus Gland
B-Lymphocytes
T-Lymphocytes
Immunoglobulin Heavy Chains
Mutation Rate
Helper-Inducer T-Lymphocytes
Exons
Phenotype
Antigens
Antibodies
Proteins

Keywords

  • Affinity maturation
  • Antibody-secreting cells
  • Immunoglobulin
  • T-cell repertoire
  • Thymus

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy

Cite this

Abuattieh, M., Bender, D., Liu, E., Wettstein, P., Platt, J. L., & Cascalho, M. (2012). Affinity maturation of antibodies requires integrity of the adult thymus. European Journal of Immunology, 42(2), 500-510. https://doi.org/10.1002/eji.201141889

Affinity maturation of antibodies requires integrity of the adult thymus. / Abuattieh, Mouhammed; Bender, Diane; Liu, Esther; Wettstein, Peter; Platt, Jeffrey L.; Cascalho, Marilia.

In: European Journal of Immunology, Vol. 42, No. 2, 02.2012, p. 500-510.

Research output: Contribution to journalArticle

Abuattieh, M, Bender, D, Liu, E, Wettstein, P, Platt, JL & Cascalho, M 2012, 'Affinity maturation of antibodies requires integrity of the adult thymus', European Journal of Immunology, vol. 42, no. 2, pp. 500-510. https://doi.org/10.1002/eji.201141889
Abuattieh M, Bender D, Liu E, Wettstein P, Platt JL, Cascalho M. Affinity maturation of antibodies requires integrity of the adult thymus. European Journal of Immunology. 2012 Feb;42(2):500-510. https://doi.org/10.1002/eji.201141889
Abuattieh, Mouhammed ; Bender, Diane ; Liu, Esther ; Wettstein, Peter ; Platt, Jeffrey L. ; Cascalho, Marilia. / Affinity maturation of antibodies requires integrity of the adult thymus. In: European Journal of Immunology. 2012 ; Vol. 42, No. 2. pp. 500-510.
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