Aequorin luminescence: relation of light emission to calcium concentration - a calcium independent component

D. G. Allen, J. R. Blinks, F. G. Prendergast

Research output: Contribution to journalArticle

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Abstract

Light emission from the calcium-sensitive bioluminescent protein aequorin was measured at calcium ion concentrations of 10-9 to 10-2 molar. At very low Ca2+ concentrations, light emission is independent of calcium ion concentration. The maximum slope of the log-log plot of light as a function of calcium ion concentration is about 2.5. The complete relation is well described by a two-state model involving three calcium-binding sites.

Original languageEnglish (US)
Pages (from-to)996-998
Number of pages3
JournalScience
Volume195
Issue number4282
StatePublished - 1977

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Aequorin
Luminescence
Calcium
Light
Ions
Luminescent Proteins
Binding Sites

ASJC Scopus subject areas

  • General

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Allen, D. G., Blinks, J. R., & Prendergast, F. G. (1977). Aequorin luminescence: relation of light emission to calcium concentration - a calcium independent component. Science, 195(4282), 996-998.

Aequorin luminescence : relation of light emission to calcium concentration - a calcium independent component. / Allen, D. G.; Blinks, J. R.; Prendergast, F. G.

In: Science, Vol. 195, No. 4282, 1977, p. 996-998.

Research output: Contribution to journalArticle

Allen, DG, Blinks, JR & Prendergast, FG 1977, 'Aequorin luminescence: relation of light emission to calcium concentration - a calcium independent component', Science, vol. 195, no. 4282, pp. 996-998.
Allen, D. G. ; Blinks, J. R. ; Prendergast, F. G. / Aequorin luminescence : relation of light emission to calcium concentration - a calcium independent component. In: Science. 1977 ; Vol. 195, No. 4282. pp. 996-998.
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