TY - JOUR
T1 - Adjuvant effect of Lactobacillus casei in a mouse model of gluten sensitivity
AU - D'Arienzo, Rossana
AU - Maurano, Francesco
AU - Luongo, Diomira
AU - Mazzarella, Giuseppe
AU - Stefanile, Rosita
AU - Troncone, Riccardo
AU - Auricchio, Salvatore
AU - Ricca, Ezio
AU - David, Chella
AU - Rossi, Mauro
N1 - Funding Information:
The work was supported by grants from Consiglio Nazionale delle Ricerche (CNR) and European Laboratory for the Investigation of Food-Induced Diseases (ELFID). RD is the recipient of a doctoral fellowship from the University Federico II of Naples. The authors acknowledge the technical assistance of Loredana Arciuolo for the management of the mouse colony.
PY - 2008/8/15
Y1 - 2008/8/15
N2 - Probiotic strains have been reported to exert immunomodulatory activities in the gut-associated lymphoid tissue. In this study we explored the effect of Lactobacillus casei in transgenic mice expressing the human DQ8 heterodimer, a HLA molecule linked to Celiac Disease (CD). DQ8 mice, mucosally immunized with the gluten component gliadin, mounted an intestinal Th1-like response as observed in CD, without developing enteropathy. Co-administration of L. casei in sensitized mice specifically enhanced the gliadin-specific response mediated by CD4+ T cells. Notably, both a strong increase of the gliadin-specific IFNγ expression and a pro-inflammatory polarization of the cytokine milieu in the small intestinal mucosa were associated to the presence of the probiotic strain. However, this condition did not bring on any mucosal alteration. These findings suggest that the gliadin-specific enteropathy is not merely related to the HLA DQ8-restricted massive production of IFNγ, but additional parameters are involved. Moreover, our data imply that the intrinsic adjuvanticity of L. casei can be exploited to further enhance both mucosal and systemic T cell-mediated responses.
AB - Probiotic strains have been reported to exert immunomodulatory activities in the gut-associated lymphoid tissue. In this study we explored the effect of Lactobacillus casei in transgenic mice expressing the human DQ8 heterodimer, a HLA molecule linked to Celiac Disease (CD). DQ8 mice, mucosally immunized with the gluten component gliadin, mounted an intestinal Th1-like response as observed in CD, without developing enteropathy. Co-administration of L. casei in sensitized mice specifically enhanced the gliadin-specific response mediated by CD4+ T cells. Notably, both a strong increase of the gliadin-specific IFNγ expression and a pro-inflammatory polarization of the cytokine milieu in the small intestinal mucosa were associated to the presence of the probiotic strain. However, this condition did not bring on any mucosal alteration. These findings suggest that the gliadin-specific enteropathy is not merely related to the HLA DQ8-restricted massive production of IFNγ, but additional parameters are involved. Moreover, our data imply that the intrinsic adjuvanticity of L. casei can be exploited to further enhance both mucosal and systemic T cell-mediated responses.
KW - Intestinal mucosa
KW - Probiotic
KW - Transgenic mice
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U2 - 10.1016/j.imlet.2008.04.006
DO - 10.1016/j.imlet.2008.04.006
M3 - Article
C2 - 18547649
AN - SCOPUS:47149083075
SN - 0165-2478
VL - 119
SP - 78
EP - 83
JO - Immunology Letters
JF - Immunology Letters
IS - 1-2
ER -