Abstract
Objective To establish and characterize a novel domestic porcine model of obesity. Methods Fourteen domestic pigs were fed normal (lean, n = 7) or high-fat/high-fructose diet (obese, n = 7) for 16 weeks. Subcutaneous abdominal adipose tissue biopsies were obtained after 8, 12, and 16 weeks of diet, and pericardial adipose tissue after 16 weeks, for assessments of adipocyte size, fibrosis, and inflammation. Adipose tissue volume and cardiac function were studied with multidetector computed tomography, and oxygenation was studied with magnetic resonance imaging. Plasma lipids profile, insulin resistance, and markers of inflammation were evaluated. Results Compared with lean pigs, obese pigs had elevated cholesterol and triglyceride levels, blood pressure, and insulin resistance. Both abdominal and pericardial fat volume increased in obese pigs after 16 weeks. In abdominal subcutaneous adipose tissue, adipocyte size and both tumor necrosis factor (TNF)-α expression progressively increased. Macrophage infiltration showed in both abdominal and pericardial adipose tissues. Circulating TNF-α increased in obese pigs only at 16 weeks. Compared with lean pigs, obese pigs had similar global cardiac function, but myocardial perfusion and oxygenation were significantly impaired. Conclusions A high-fat/high-fructose diet induces in domestic pigs many characteristics of metabolic syndrome, which is useful for investigating the effects of the obesity.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 399-407 |
| Number of pages | 9 |
| Journal | Obesity |
| Volume | 23 |
| Issue number | 2 |
| DOIs | |
| State | Published - Feb 1 2015 |
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ASJC Scopus subject areas
- Endocrinology
- Medicine (miscellaneous)
- Endocrinology, Diabetes and Metabolism
- Nutrition and Dietetics
Cite this
Adipose tissue remodeling in a novel domestic porcine model of diet-induced obesity. / Pawar, Aditya S.; Zhu, Xiang Yang; Eirin, Alfonso; Tang, Hui; Jordan, Kyra L.; Woollard, John R.; Lerman, Amir; Lerman, Lilach O.
In: Obesity, Vol. 23, No. 2, 01.02.2015, p. 399-407.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Adipose tissue remodeling in a novel domestic porcine model of diet-induced obesity
AU - Pawar, Aditya S.
AU - Zhu, Xiang Yang
AU - Eirin, Alfonso
AU - Tang, Hui
AU - Jordan, Kyra L.
AU - Woollard, John R.
AU - Lerman, Amir
AU - Lerman, Lilach O
PY - 2015/2/1
Y1 - 2015/2/1
N2 - Objective To establish and characterize a novel domestic porcine model of obesity. Methods Fourteen domestic pigs were fed normal (lean, n = 7) or high-fat/high-fructose diet (obese, n = 7) for 16 weeks. Subcutaneous abdominal adipose tissue biopsies were obtained after 8, 12, and 16 weeks of diet, and pericardial adipose tissue after 16 weeks, for assessments of adipocyte size, fibrosis, and inflammation. Adipose tissue volume and cardiac function were studied with multidetector computed tomography, and oxygenation was studied with magnetic resonance imaging. Plasma lipids profile, insulin resistance, and markers of inflammation were evaluated. Results Compared with lean pigs, obese pigs had elevated cholesterol and triglyceride levels, blood pressure, and insulin resistance. Both abdominal and pericardial fat volume increased in obese pigs after 16 weeks. In abdominal subcutaneous adipose tissue, adipocyte size and both tumor necrosis factor (TNF)-α expression progressively increased. Macrophage infiltration showed in both abdominal and pericardial adipose tissues. Circulating TNF-α increased in obese pigs only at 16 weeks. Compared with lean pigs, obese pigs had similar global cardiac function, but myocardial perfusion and oxygenation were significantly impaired. Conclusions A high-fat/high-fructose diet induces in domestic pigs many characteristics of metabolic syndrome, which is useful for investigating the effects of the obesity.
AB - Objective To establish and characterize a novel domestic porcine model of obesity. Methods Fourteen domestic pigs were fed normal (lean, n = 7) or high-fat/high-fructose diet (obese, n = 7) for 16 weeks. Subcutaneous abdominal adipose tissue biopsies were obtained after 8, 12, and 16 weeks of diet, and pericardial adipose tissue after 16 weeks, for assessments of adipocyte size, fibrosis, and inflammation. Adipose tissue volume and cardiac function were studied with multidetector computed tomography, and oxygenation was studied with magnetic resonance imaging. Plasma lipids profile, insulin resistance, and markers of inflammation were evaluated. Results Compared with lean pigs, obese pigs had elevated cholesterol and triglyceride levels, blood pressure, and insulin resistance. Both abdominal and pericardial fat volume increased in obese pigs after 16 weeks. In abdominal subcutaneous adipose tissue, adipocyte size and both tumor necrosis factor (TNF)-α expression progressively increased. Macrophage infiltration showed in both abdominal and pericardial adipose tissues. Circulating TNF-α increased in obese pigs only at 16 weeks. Compared with lean pigs, obese pigs had similar global cardiac function, but myocardial perfusion and oxygenation were significantly impaired. Conclusions A high-fat/high-fructose diet induces in domestic pigs many characteristics of metabolic syndrome, which is useful for investigating the effects of the obesity.
UR - http://www.scopus.com/inward/record.url?scp=84922688117&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84922688117&partnerID=8YFLogxK
U2 - 10.1002/oby.20971
DO - 10.1002/oby.20971
M3 - Article
C2 - 25627626
AN - SCOPUS:84922688117
VL - 23
SP - 399
EP - 407
JO - Obesity
JF - Obesity
SN - 1930-7381
IS - 2
ER -