TY - JOUR
T1 - Adipose Tissue Inflammation Is Not Related to Adipose Insulin Resistance in Humans
AU - De Ycaza, Ana Elena Espinosa
AU - Søndergaard, Esben
AU - Morgan-Bathke, Maria
AU - Lytle, Kelli
AU - Delivanis, Danae A.
AU - Ramos, Paola
AU - Carranza Leon, Barbara Gisella
AU - Jensen, Michael D.
N1 - Publisher Copyright:
© 2022 by the American Diabetes Association.
PY - 2022/3
Y1 - 2022/3
N2 - The role of adipose tissue (AT) inflammation in AT function in humans is unclear. We tested whether AT macrophage (ATM) content, cytokine gene expression, and senescent cell burden (markers of AT inflammation) predict AT insulin resistance measured as the insulin concentration that suppresses lipolysis by 50% (IC50). We studied 86 volunteers with normal weight or obesity at baseline and a subgroup of 25 volunteers with obesity before and after weight loss. There was a strong positive relationship between IC50 and abdominal subcutaneous and femoral fat cell size (FCS). The positive, univariate relationships between IC50 and abdominal AT inflammatory markers CD68, CD14, CD206 ATM/100 adipocytes, senescent cells, IL-6, and TNF-a mRNA were not significant after adjustment for FCS. A 10% weight loss significantly reduced IC50; however, there was no reduction in adipose ATM content, senescent cells, or cytokine gene expression. Our study suggests that commonly used markers of AT inflammation are not causally linked to AT insulin resistance, whereas FCS is a strong predictor of AT insulin resistance with respect to lipolysis.
AB - The role of adipose tissue (AT) inflammation in AT function in humans is unclear. We tested whether AT macrophage (ATM) content, cytokine gene expression, and senescent cell burden (markers of AT inflammation) predict AT insulin resistance measured as the insulin concentration that suppresses lipolysis by 50% (IC50). We studied 86 volunteers with normal weight or obesity at baseline and a subgroup of 25 volunteers with obesity before and after weight loss. There was a strong positive relationship between IC50 and abdominal subcutaneous and femoral fat cell size (FCS). The positive, univariate relationships between IC50 and abdominal AT inflammatory markers CD68, CD14, CD206 ATM/100 adipocytes, senescent cells, IL-6, and TNF-a mRNA were not significant after adjustment for FCS. A 10% weight loss significantly reduced IC50; however, there was no reduction in adipose ATM content, senescent cells, or cytokine gene expression. Our study suggests that commonly used markers of AT inflammation are not causally linked to AT insulin resistance, whereas FCS is a strong predictor of AT insulin resistance with respect to lipolysis.
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U2 - 10.2337/db21-0609
DO - 10.2337/db21-0609
M3 - Article
C2 - 34857544
AN - SCOPUS:85125212453
SN - 0012-1797
VL - 71
SP - 381
EP - 393
JO - Diabetes
JF - Diabetes
IS - 3
ER -