Adiponectin, a Fat Cell Product, Influences the Earliest Lymphocyte Precursors in Bone Marrow Cultures by Activation of the Cyclooxygenase-Prostaglandin Pathway in Stromal Cells

Takafumi Yokota, C. S Reddy Meka, Taku Kouro, Kay L Medina, Hideya Igarashi, Masahiko Takahashi, Kenji Oritani, Tohru Funahashi, Yoshiaki Tomiyama, Yuji Matsuzawa, Paul W. Kincade

Research output: Contribution to journalArticle

99 Citations (Scopus)

Abstract

Adiponectin, an adipocyte-derived hormone, is attracting considerable interest as a potential drug for diabetes and obesity. Originally cloned from human s.c. fat, the protein is also found in bone marrow fat cells and has an inhibitory effect on adipocyte differentiation. The aim of the present study is to explore possible influences on lymphohematopoiesis. Recombinant adiponectin strongly inhibited B lymphopoiesis in long-term bone marrow cultures, but only when stromal cells were present and only when cultures were initiated with the earliest category of lymphocyte precursors. Cyclooxygenase inhibitors abrogated the response of early lymphoid progenitors to adiponectin in stromal cell-containing cultures. Furthermore, PGE2, a major product of cyclooxygenase-2 activity, had a direct inhibitory influence on purified hematopoietic cells, suggesting a possible mechanism of adiponectin action in culture. In contrast to lymphopoiesis, myelopoiesis was slightly enhanced in adiponectin-treated bone marrow cultures, and even when cultures were initiated with single lymphomyeloid progenitors. Finally, human B lymphopoiesis was also sensitive to adiponectin in stromal cell cocultures. These results suggest that adiponectin can negatively and selectively influence lymphopoiesis through induction of PG synthesis. They also indicate ways that adipocytes in bone marrow can contribute to regulation of blood cell formation.

Original languageEnglish (US)
Pages (from-to)5091-5099
Number of pages9
JournalJournal of Immunology
Volume171
Issue number10
StatePublished - Nov 15 2003
Externally publishedYes

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Adiponectin
Prostaglandin-Endoperoxide Synthases
Stromal Cells
Adipocytes
Lymphopoiesis
Bone Marrow
Lymphocytes
Myelopoiesis
Cyclooxygenase Inhibitors
Cyclooxygenase 2
Coculture Techniques
Dinoprostone
Bone Marrow Cells
Blood Cells
Cell Culture Techniques
Obesity
Fats
Hormones
Pharmaceutical Preparations
Proteins

ASJC Scopus subject areas

  • Immunology

Cite this

Adiponectin, a Fat Cell Product, Influences the Earliest Lymphocyte Precursors in Bone Marrow Cultures by Activation of the Cyclooxygenase-Prostaglandin Pathway in Stromal Cells. / Yokota, Takafumi; Meka, C. S Reddy; Kouro, Taku; Medina, Kay L; Igarashi, Hideya; Takahashi, Masahiko; Oritani, Kenji; Funahashi, Tohru; Tomiyama, Yoshiaki; Matsuzawa, Yuji; Kincade, Paul W.

In: Journal of Immunology, Vol. 171, No. 10, 15.11.2003, p. 5091-5099.

Research output: Contribution to journalArticle

Yokota, T, Meka, CSR, Kouro, T, Medina, KL, Igarashi, H, Takahashi, M, Oritani, K, Funahashi, T, Tomiyama, Y, Matsuzawa, Y & Kincade, PW 2003, 'Adiponectin, a Fat Cell Product, Influences the Earliest Lymphocyte Precursors in Bone Marrow Cultures by Activation of the Cyclooxygenase-Prostaglandin Pathway in Stromal Cells', Journal of Immunology, vol. 171, no. 10, pp. 5091-5099.
Yokota, Takafumi ; Meka, C. S Reddy ; Kouro, Taku ; Medina, Kay L ; Igarashi, Hideya ; Takahashi, Masahiko ; Oritani, Kenji ; Funahashi, Tohru ; Tomiyama, Yoshiaki ; Matsuzawa, Yuji ; Kincade, Paul W. / Adiponectin, a Fat Cell Product, Influences the Earliest Lymphocyte Precursors in Bone Marrow Cultures by Activation of the Cyclooxygenase-Prostaglandin Pathway in Stromal Cells. In: Journal of Immunology. 2003 ; Vol. 171, No. 10. pp. 5091-5099.
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