TY - JOUR
T1 - Adipogenesis in a murine bone marrow stromal cell line capable of supporting B lineage lymphocyte growth and proliferation
T2 - Biochemical and molecular characterization
AU - Gimble, Jeffrey Martin
AU - Dorheim, Marie‐Annette ‐A
AU - Cheng, Qi
AU - Medina, Kay
AU - Wang, Chi‐Sun ‐S
AU - Jones, Robert
AU - Koren, Eugen
AU - Pietrangeli, Carolynn
AU - Kincade, Paul W.
PY - 1990/2
Y1 - 1990/2
N2 - Recent advances in long‐term bone marrow (BM) culture techniques have allowed investigators to dissect cellular components responsible for lympho‐hematopoiesis. Consequently, a number of “stromal” cell clones have been developed which are capable of supporting B lineage lymphocyte growth and proliferation in vitro by direct cell‐cell interactions and the release of cytokines. While much work has focused on the support function of these cells, questions remain regarding their own differentiation potential. We have examined adipo‐genesis in the cloned BM stromal cell, BMS2. The presence of hydrocortisone, methylisobutylxanthine, or 30% fetal calf serum each accelerated adipocyte differentiation. This process was accompanied by the accumulation of triglycerides and cholesterol esters along with the induction of adipocyte‐specific enzymes. Likewise, the steady‐state level of mRNA transcripts increased for genes related to lipid metabolism. However, the pattern of mRNA expression in BMS2 adipocytes differed from that of a well‐established, pre‐adipocyte cell line, 3T3‐L1, with respect to the following genes: glycerol phosphate dehydrogenase, CAAT/enhancer binding protein and angiotensinogen. Adipocyte BMS2 cells retailed the ability to support stromal cell‐dependent B lineage lymphocytes in methylcellulose assays. The adipocytes continued to express macrophagecolony‐stimulating factor mRNA constitutively and interleukin 6 mRNA in an inducible manner, similar to the BMS2 pre‐adipocytes. Together, these data document a close developmental relationship between a specialized fibroblasts and adipocytes in the BM and suggest that adipocyte stromal cells may play an active role in lympho‐hematopoiesis.
AB - Recent advances in long‐term bone marrow (BM) culture techniques have allowed investigators to dissect cellular components responsible for lympho‐hematopoiesis. Consequently, a number of “stromal” cell clones have been developed which are capable of supporting B lineage lymphocyte growth and proliferation in vitro by direct cell‐cell interactions and the release of cytokines. While much work has focused on the support function of these cells, questions remain regarding their own differentiation potential. We have examined adipo‐genesis in the cloned BM stromal cell, BMS2. The presence of hydrocortisone, methylisobutylxanthine, or 30% fetal calf serum each accelerated adipocyte differentiation. This process was accompanied by the accumulation of triglycerides and cholesterol esters along with the induction of adipocyte‐specific enzymes. Likewise, the steady‐state level of mRNA transcripts increased for genes related to lipid metabolism. However, the pattern of mRNA expression in BMS2 adipocytes differed from that of a well‐established, pre‐adipocyte cell line, 3T3‐L1, with respect to the following genes: glycerol phosphate dehydrogenase, CAAT/enhancer binding protein and angiotensinogen. Adipocyte BMS2 cells retailed the ability to support stromal cell‐dependent B lineage lymphocytes in methylcellulose assays. The adipocytes continued to express macrophagecolony‐stimulating factor mRNA constitutively and interleukin 6 mRNA in an inducible manner, similar to the BMS2 pre‐adipocytes. Together, these data document a close developmental relationship between a specialized fibroblasts and adipocytes in the BM and suggest that adipocyte stromal cells may play an active role in lympho‐hematopoiesis.
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U2 - 10.1002/eji.1830200222
DO - 10.1002/eji.1830200222
M3 - Article
C2 - 2178944
AN - SCOPUS:0025012627
VL - 20
SP - 379
EP - 387
JO - European Journal of Immunology
JF - European Journal of Immunology
SN - 0014-2980
IS - 2
ER -