TY - JOUR
T1 - Adipocyte Proteins and Storage of Endogenous Fatty Acids in Visceral and Subcutaneous Adipose Tissue in Severe Obesity
AU - Lytle, Kelli A.
AU - Bush, Nikki C.
AU - Triay, Jessica M.
AU - Kellogg, Todd A.
AU - Kendrick, Michael L.
AU - Swain, James M.
AU - Gathaiya, Nicola W.
AU - Hames, Kazanna C.
AU - Jensen, Michael D.
N1 - Funding Information:
This work was supported by grants DK40484, DK45343, DK50456, and the National Center for Research Resources and the National Center for Advancing Translational Sciences, National Institutes of Health (NIH), through grant number 1 UL1 RR024150‐01and UL1 TR000135. KAL, NCB, and KCH received fellowship funding from the NIH training grant T32‐DK07352 and the American Diabetes Association fellowship grant 7‐112‐MN‐36. The content is solely the responsibility of the authors and does not necessarily represent the official views of the NIH.
Publisher Copyright:
© 2021 The Obesity Society.
PY - 2021/6
Y1 - 2021/6
N2 - Objective: This study tested whether substrate concentrations or fatty acid storage proteins predict storage of endogenous lipids in visceral adipose tissue (VAT) and upper body subcutaneous adipose tissue (UBSQ) fat. Methods: The day prior to surgery, 25 patients undergoing bariatric procedures received an infusion of autologous [1-14C]triolein-labeled very low-density lipoprotein (VLDL) particles, and during surgery, they received a continuous [U-13C]palmitate infusion/bolus [9,10-3H]palmitate tracer. VAT and UBSQ fat were collected to measure VLDL-triglyceride (TG) storage, direct free fatty acid (FFA) storage rates, CD36 content, lipoprotein lipase (LPL), acyl-CoA synthetase, diacylglycerol acetyl-transferase, and glycerol-3-phosphate acyltransferase activities. Results: Storage of VLDL-TG and FFA-palmitate in UBSQ and VAT was not different. Plasma palmitate concentrations correlated with palmitate storage rates in UBSQ and VAT (r = 0.46, P = 0.02 and r = 0.46, P = 0.02, respectively). In VAT, VLDL-TG storage was correlated with VLDL concentrations (r = 0.53, P < 0.009) and LPL (r = 0.42, P < 0.05). In UBSQ, VLDL-TG storage was correlated with LPL (r = 0.42, P < 0.05). CD36, acyl-CoA synthetase, glycerol-3-phosphate acyltransferase, and diacylglycerol acetyl-transferase were not correlated with VLDL-TG or palmitate storage. Conclusions: Adipose storage of VLDL-TG is predicted by VLDL-TG concentrations and LPL; FFA concentrations predict direct adipose tissue FFA storage rates.
AB - Objective: This study tested whether substrate concentrations or fatty acid storage proteins predict storage of endogenous lipids in visceral adipose tissue (VAT) and upper body subcutaneous adipose tissue (UBSQ) fat. Methods: The day prior to surgery, 25 patients undergoing bariatric procedures received an infusion of autologous [1-14C]triolein-labeled very low-density lipoprotein (VLDL) particles, and during surgery, they received a continuous [U-13C]palmitate infusion/bolus [9,10-3H]palmitate tracer. VAT and UBSQ fat were collected to measure VLDL-triglyceride (TG) storage, direct free fatty acid (FFA) storage rates, CD36 content, lipoprotein lipase (LPL), acyl-CoA synthetase, diacylglycerol acetyl-transferase, and glycerol-3-phosphate acyltransferase activities. Results: Storage of VLDL-TG and FFA-palmitate in UBSQ and VAT was not different. Plasma palmitate concentrations correlated with palmitate storage rates in UBSQ and VAT (r = 0.46, P = 0.02 and r = 0.46, P = 0.02, respectively). In VAT, VLDL-TG storage was correlated with VLDL concentrations (r = 0.53, P < 0.009) and LPL (r = 0.42, P < 0.05). In UBSQ, VLDL-TG storage was correlated with LPL (r = 0.42, P < 0.05). CD36, acyl-CoA synthetase, glycerol-3-phosphate acyltransferase, and diacylglycerol acetyl-transferase were not correlated with VLDL-TG or palmitate storage. Conclusions: Adipose storage of VLDL-TG is predicted by VLDL-TG concentrations and LPL; FFA concentrations predict direct adipose tissue FFA storage rates.
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U2 - 10.1002/oby.23149
DO - 10.1002/oby.23149
M3 - Article
AN - SCOPUS:85104774564
VL - 29
SP - 1014
EP - 1021
JO - Obesity
JF - Obesity
SN - 1930-7381
IS - 6
ER -