Adipocyte macrophage colony-stimulating factor is a mediator of adipose tissue growth

James A. Levine, Michael Dennis Jensen, Norman L. Eberhardt, Timothy O'Brien

Research output: Contribution to journalArticle

111 Citations (Scopus)

Abstract

Adipose tissue growth results from de novo adipocyte recruitment (hyperplasia) and increased size of preexisting adipocytes. Adipocyte hyperplasia accounts for the several-fold increase in adipose tissue mass that occurs throughout life, yet the mechanism of adipocyte hyperplasia is unknown. We studied the potential of macrophage colony-stimulating factor (MCSF) to mediate adipocyte hyperplasia because of the profound effects MCSF exerts on pluripotent cell recruitment and differentiation in other tissues. We found that MCSF mRNA and protein were expressed by human adipocytes and that adipocyte MCSF expression was upregulated in rapidly growing adipose tissue that encircled acutely inflamed bowel and in adipose tissue from humans gaining weight (4-7 kg) with overfeeding. Localized overexpression of adipocyte MCSF was then induced in rabbit subcutaneous adipose tissue in vivo using adenoviral-mediated gene transfer. Successful overexpression of MCSF was associated with 16-fold increases in adipose tissue growth compared with a control adenovirus expressing β-galactosidase. This occurred in the absence of increased cell size and in the presence of increased nuclear staining for MIB-1, a marker of proliferation. We conclude that MCSF participates in adipocyte hyperplasia and the physiological regulation of adipose tissue growth.

Original languageEnglish (US)
Pages (from-to)1557-1564
Number of pages8
JournalJournal of Clinical Investigation
Volume101
Issue number8
StatePublished - Apr 15 1998

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Macrophage Colony-Stimulating Factor
Adipocytes
Adipose Tissue
Growth
Hyperplasia
Galactosidases
Subcutaneous Fat
Cell Size
Adenoviridae
Cell Differentiation
Staining and Labeling
Rabbits
Weights and Measures
Messenger RNA

Keywords

  • Adenovirus
  • Adipose tissue
  • Gene transfer
  • Macrophage colony-stimulating factor
  • Obesity

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Adipocyte macrophage colony-stimulating factor is a mediator of adipose tissue growth. / Levine, James A.; Jensen, Michael Dennis; Eberhardt, Norman L.; O'Brien, Timothy.

In: Journal of Clinical Investigation, Vol. 101, No. 8, 15.04.1998, p. 1557-1564.

Research output: Contribution to journalArticle

Levine, James A. ; Jensen, Michael Dennis ; Eberhardt, Norman L. ; O'Brien, Timothy. / Adipocyte macrophage colony-stimulating factor is a mediator of adipose tissue growth. In: Journal of Clinical Investigation. 1998 ; Vol. 101, No. 8. pp. 1557-1564.
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