Adhesion, internalization and metabolism of calcium oxalate monohydrate crystals by renal epithelial cells

John C Lieske, Rebbecca Norris, Hewson Swift, F. Gary Toback

Research output: Contribution to journalArticle

96 Citations (Scopus)

Abstract

The interaction between crystals that nucleate in the nephron lumen and tubular cells could be an important determinant of renal calcification. Kidney epithelial cells in monolayer culture (BSC-1 line), used to model the tubule, rapidly bound and internalized crystals of calcium oxalate monohydrate (COM), the most common constituent of renal stones. Transmission and scanning electron microscopy, enzyme histochemistry, and kinetic analysis of [14C]-labeled crystals were used to study the interaction between renal cells and COM crystals. Electron microscopy revealed that adherent crystals on the apical cell surface can serve as sites for aggregation of additional crystals. Enhanced binding of exogenous crystals to plasma membrane domains overlying internalized crystals was observed for at least 24 hours after the initial cell-crystal interaction. Following internalization, crystals appeared to dissolve within lysosomal inclusion bodies during the ensuing five to seven weeks. Over this time, many cells still containing crystals clustered together in the monolayer. These observations suggest that adhesion and internalization can promote crystal retention in the nephron, whereas intracellular dissolution of crystals may serve as an important, hitherto unrecognized defense against pathologic renal calcification.

Original languageEnglish (US)
Pages (from-to)1291-1301
Number of pages11
JournalKidney International
Volume52
Issue number5
StatePublished - 1997
Externally publishedYes

Fingerprint

Calcium Oxalate
Epithelial Cells
Kidney
Nephrons
Calcinosis
Scanning Transmission Electron Microscopy
Inclusion Bodies
Cell Communication
Electron Microscopy
Cell Membrane
Enzymes

Keywords

  • Acid phosphatase
  • Brushite
  • BSC-1 cells
  • Calcium oxalate crystals
  • Lysosome
  • Nephrolithiasis

ASJC Scopus subject areas

  • Nephrology

Cite this

Adhesion, internalization and metabolism of calcium oxalate monohydrate crystals by renal epithelial cells. / Lieske, John C; Norris, Rebbecca; Swift, Hewson; Toback, F. Gary.

In: Kidney International, Vol. 52, No. 5, 1997, p. 1291-1301.

Research output: Contribution to journalArticle

Lieske, John C ; Norris, Rebbecca ; Swift, Hewson ; Toback, F. Gary. / Adhesion, internalization and metabolism of calcium oxalate monohydrate crystals by renal epithelial cells. In: Kidney International. 1997 ; Vol. 52, No. 5. pp. 1291-1301.
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