TY - JOUR
T1 - Adenoviral-mediated overexpression of catalase inhibits endothelial cell proliferation
AU - Zanetti, Michela
AU - Katusic, Zvonimir S.
AU - O'Brien, Timothy
N1 - Copyright:
Copyright 2020 Elsevier B.V., All rights reserved.
PY - 2002/12/1
Y1 - 2002/12/1
N2 - Although hydrogen peroxide (H2O2) induces proliferation of vascular smooth muscle cells, its role in endothelial cell proliferation is unclear. Our aim was to study the role of hydrogen peroxide in endothelial cell proliferation by overexpressing catalase. Human aortic endothelial cells were transduced with adenoviral vectors encoding β-galactosidase (Adβgal) or catalase (AdCat) or were exposed to diluent alone (control). Transgene expression was demonstrated by β-galactosidase staining, Western analysis, and significantly increased enzyme activity in AdCat-transduced cells. Overexpression of catalase decreased DNA synthesis in AdCat compared with control and Adβgal-transduced cells (536.8 ± 31 vs. 1,875.1 ± 132.9 vs. 1,347.5 ± 93.7 dpm/well, respectively; P < 0.05 vs. control and Adβgal). Six days after transduction with AdCat (multiplicity of infection = 50), cell numbers were significantly reduced (AdCat: 38 ± 1.8% of cell counts in control, P < 0.05; and 45 ± 2% of cell count in Adβgal, P < 0.05). Incubation with aminotriazole 10 mmol/l, an inhibitor of catalase, prevented this effect. The number of apoptotic cells was increased one- and threefold 2 and 4 days, respectively, after transduction with AdCat. Exogenous administration of low concentrations of H2O2 (50 μM) significantly increased cell proliferation, whereas it was inhibited by higher concentrations. These results suggest that H2O2 is an important modulator of endothelial cell proliferation.
AB - Although hydrogen peroxide (H2O2) induces proliferation of vascular smooth muscle cells, its role in endothelial cell proliferation is unclear. Our aim was to study the role of hydrogen peroxide in endothelial cell proliferation by overexpressing catalase. Human aortic endothelial cells were transduced with adenoviral vectors encoding β-galactosidase (Adβgal) or catalase (AdCat) or were exposed to diluent alone (control). Transgene expression was demonstrated by β-galactosidase staining, Western analysis, and significantly increased enzyme activity in AdCat-transduced cells. Overexpression of catalase decreased DNA synthesis in AdCat compared with control and Adβgal-transduced cells (536.8 ± 31 vs. 1,875.1 ± 132.9 vs. 1,347.5 ± 93.7 dpm/well, respectively; P < 0.05 vs. control and Adβgal). Six days after transduction with AdCat (multiplicity of infection = 50), cell numbers were significantly reduced (AdCat: 38 ± 1.8% of cell counts in control, P < 0.05; and 45 ± 2% of cell count in Adβgal, P < 0.05). Incubation with aminotriazole 10 mmol/l, an inhibitor of catalase, prevented this effect. The number of apoptotic cells was increased one- and threefold 2 and 4 days, respectively, after transduction with AdCat. Exogenous administration of low concentrations of H2O2 (50 μM) significantly increased cell proliferation, whereas it was inhibited by higher concentrations. These results suggest that H2O2 is an important modulator of endothelial cell proliferation.
KW - Apoptosis
KW - Hydrogen peroxide
UR - http://www.scopus.com/inward/record.url?scp=0036889886&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0036889886&partnerID=8YFLogxK
U2 - 10.1152/ajpheart.00358.2001
DO - 10.1152/ajpheart.00358.2001
M3 - Article
C2 - 12427601
AN - SCOPUS:0036889886
VL - 283
SP - H2620-H2626
JO - American Journal of Physiology
JF - American Journal of Physiology
SN - 0363-6135
IS - 6 52-6
ER -