Adenoviral-mediated gene transfer to human adipocytes in vitro, and human adipose tissue ex vivo and rabbit femoral adipose tissue in vivo

J. A. Levine, N. L. Eberhardt, M. D. Jensen, T. O'Brien

Research output: Contribution to journalArticle

3 Scopus citations

Abstract

Adenoviral-mediated gene transfer has proven useful in several organ systems to understand gene action and to provide a potential therapeutic modality for localized, organ-specific gene overexpression. However, the application of adenoviral-mediated gene transfer to adipocytes and adipose tissue has not been evaluated. We evaluated the feasibility of in vitro and ex vivo transfer of the β-galactosidase gene to human adipocytes and adipose tissue by means of adenoviral vectors. The efficiency (percentage of cells transduced) of adenoviral-mediated gene transfer of the β-galactosidase gene to human adipocytes in vitro and to human adipose tissue ex vivo was 21±3% and 14±3%, respectively. Adenoviral-mediated gene transfer in a rabbit femoral adipose tissue was also demonstrated in vivo. Adenoviral-mediated gene transfer may facilitate studies on understanding the biology of adipocytes and provide a potential tool for the modulation of adipocyte function in vivo and thereby for the treatment of obesity.

Original languageEnglish (US)
Pages (from-to)569-572
Number of pages4
JournalJournal of Nutritional Science and Vitaminology
Volume44
Issue number4
DOIs
StatePublished - Jan 1 1998

Keywords

  • Adenovirus
  • Adipose tissue
  • Gene transfer
  • Obesity

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Nutrition and Dietetics

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