Adenoviral mediated delivery of FAS ligand to arthritic joints causes extensive apoptosis in the synovial lining

Qingping Yao, Joseph C. Glorioso, Christopher H Evans, Paul D. Robbins, Imre Kovesdi, Thomas J. Oligino, Steven C. Ghivizzani

Research output: Contribution to journalArticle

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Abstract

Background: Rheumatoid arthritis (RA) is an autoimmune disease where the synovial lining layer of the joint becomes thickened, hypercellular, and highly aggressive. Invading synovial tissue erodes cartilage and subchondral bone and leads to loss of joint function. FasL, a cell-surface molecule on activated T-cells interacts with its receptor, Fas, to induce apoptosis in target cells. We addressed the feasibility of using adenoviral gene transfer of FasL therapeutically to mediate apoptosis in arthritic joints similar in size to the small joints of the hands and feet that are the primary sites of RA in humans. Methods: Adenoviral vectors were used to transfer FasL and LacZ cDNAs into human RA and rabbit synovial fibroblasts in culture where apoptosis was evaluated using MTT and TUNEL analyses. The ability of Ad.FasL to mediate synovial apoptosis in vivo was then addressed in an IL-1-induced arthritis model in the rabbit knee. Results: In culture, delivery of FasL was found to efficiently induce apoptosis in both human RA and rabbit synovial fibroblasts. The ability of Ad.FasL to induce synovial apoptosis was then evaluated in rabbit knee joints. 24 h after intra-articular injection of 10 11 Ad.FasL particles, large regions of synovial tissue were observed histologically consisting primarily of fibrous matrix and cellular debris. TUNEL staining of corresponding sections was highly positive for fragmented DNA. Glycosaminoglycan (GAG) synthesis from cartilage shavings from treated joints suggests that Ad.FasL does not induce significant apoptosis in resident articular chondrocytes. Conclusions: Infection of human and rabbit synovial fibroblasts with Ad.FasL results in significant apoptotic cell death in vitro. Direct intraarticular injection of Ad.FasL in the arthritic rabbit knee results in extensive apoptosis in the synovium without affecting chondrocyte viability.

Original languageEnglish (US)
Pages (from-to)210-219
Number of pages10
JournalJournal of Gene Medicine
Volume2
Issue number3
StatePublished - May 2000
Externally publishedYes

Fingerprint

Arthritis
Joints
Apoptosis
Ligands
Rabbits
Rheumatoid Arthritis
Intra-Articular Injections
Fibroblasts
In Situ Nick-End Labeling
Chondrocytes
Cartilage
Knee
Foot Joints
Hand Joints
CD95 Antigens
Synovial Membrane
Knee Joint
Glycosaminoglycans
Interleukin-1
Autoimmune Diseases

Keywords

  • Adenovirus
  • Apoptosis
  • Arthritis
  • Fas ligand
  • Gene therapy
  • Synovium

ASJC Scopus subject areas

  • Genetics

Cite this

Yao, Q., Glorioso, J. C., Evans, C. H., Robbins, P. D., Kovesdi, I., Oligino, T. J., & Ghivizzani, S. C. (2000). Adenoviral mediated delivery of FAS ligand to arthritic joints causes extensive apoptosis in the synovial lining. Journal of Gene Medicine, 2(3), 210-219.

Adenoviral mediated delivery of FAS ligand to arthritic joints causes extensive apoptosis in the synovial lining. / Yao, Qingping; Glorioso, Joseph C.; Evans, Christopher H; Robbins, Paul D.; Kovesdi, Imre; Oligino, Thomas J.; Ghivizzani, Steven C.

In: Journal of Gene Medicine, Vol. 2, No. 3, 05.2000, p. 210-219.

Research output: Contribution to journalArticle

Yao, Q, Glorioso, JC, Evans, CH, Robbins, PD, Kovesdi, I, Oligino, TJ & Ghivizzani, SC 2000, 'Adenoviral mediated delivery of FAS ligand to arthritic joints causes extensive apoptosis in the synovial lining', Journal of Gene Medicine, vol. 2, no. 3, pp. 210-219.
Yao, Qingping ; Glorioso, Joseph C. ; Evans, Christopher H ; Robbins, Paul D. ; Kovesdi, Imre ; Oligino, Thomas J. ; Ghivizzani, Steven C. / Adenoviral mediated delivery of FAS ligand to arthritic joints causes extensive apoptosis in the synovial lining. In: Journal of Gene Medicine. 2000 ; Vol. 2, No. 3. pp. 210-219.
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AU - Yao, Qingping

AU - Glorioso, Joseph C.

AU - Evans, Christopher H

AU - Robbins, Paul D.

AU - Kovesdi, Imre

AU - Oligino, Thomas J.

AU - Ghivizzani, Steven C.

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AB - Background: Rheumatoid arthritis (RA) is an autoimmune disease where the synovial lining layer of the joint becomes thickened, hypercellular, and highly aggressive. Invading synovial tissue erodes cartilage and subchondral bone and leads to loss of joint function. FasL, a cell-surface molecule on activated T-cells interacts with its receptor, Fas, to induce apoptosis in target cells. We addressed the feasibility of using adenoviral gene transfer of FasL therapeutically to mediate apoptosis in arthritic joints similar in size to the small joints of the hands and feet that are the primary sites of RA in humans. Methods: Adenoviral vectors were used to transfer FasL and LacZ cDNAs into human RA and rabbit synovial fibroblasts in culture where apoptosis was evaluated using MTT and TUNEL analyses. The ability of Ad.FasL to mediate synovial apoptosis in vivo was then addressed in an IL-1-induced arthritis model in the rabbit knee. Results: In culture, delivery of FasL was found to efficiently induce apoptosis in both human RA and rabbit synovial fibroblasts. The ability of Ad.FasL to induce synovial apoptosis was then evaluated in rabbit knee joints. 24 h after intra-articular injection of 10 11 Ad.FasL particles, large regions of synovial tissue were observed histologically consisting primarily of fibrous matrix and cellular debris. TUNEL staining of corresponding sections was highly positive for fragmented DNA. Glycosaminoglycan (GAG) synthesis from cartilage shavings from treated joints suggests that Ad.FasL does not induce significant apoptosis in resident articular chondrocytes. Conclusions: Infection of human and rabbit synovial fibroblasts with Ad.FasL results in significant apoptotic cell death in vitro. Direct intraarticular injection of Ad.FasL in the arthritic rabbit knee results in extensive apoptosis in the synovium without affecting chondrocyte viability.

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