Adenoviral gene transfer of a single-chain IL-23 induces psoriatic arthritis-like symptoms in NOD mice

Rafael R. Flores, Lana Carbo, Eun Kim, Montina Van Meter, Consuelo M.Lopez De Padilla, Jing Zhao, Debora Colangelo, Matthew J. Yousefzadeh, Luise A. Angelini, Lei Zhang, Enrico Pola, Nam Vo, Christopher H Evans, Andrea Gambotto, Laura J. Niedernhofer, Paul D. Robbins

Research output: Contribution to journalArticle

Abstract

Previously, we demonstrated that intratumoral delivery of adenoviral vector encoding single-chain (sc)IL-23 (Ad.scIL-23) was able to induce systemic antitumor immunity. Here, we examined the role of IL-23 in diabetes in nonobese diabetic mice. Intravenous delivery of Ad.scIL-23 did not accelerate the onset of hyperglycemia but instead resulted in the development of psoriatic arthritis. Ad.scIL-23-treated mice developed erythema, scales, and thickening of the skin, as well as intervertebral disc degeneration and extensive synovial hypertrophy and loss of articular cartilage in the knees. Immunological analysis revealed activation of conventional T helper type 17 cells and IL-17-producing γδ T cells along with a significant depletion and suppression of T cells in the pancreatic lymph nodes. Furthermore, treatment with anti-IL-17 antibody reduced joint and skin psoriatic arthritis pathologies. Thus, these Ad.scIL-23-treated mice represent a physiologically relevant model of psoriatic arthritis for understanding disease progression and for testing therapeutic approaches.-Flores, R. R., Carbo, L., Kim, E., Van Meter, M., De Padilla, C. M. L., Zhao, J., Colangelo, D., Yousefzadeh, M. J., Angelini, L. A., Zhang, L., Pola, E., Vo, N., Evans, C. H., Gambotto, A., Niedernhofer, L. J., Robbins, P. D. Adenoviral gene transfer of a single-chain IL-23 induces psoriatic arthritis-like symptoms in NOD mice.

Original languageEnglish (US)
Pages (from-to)9505-9515
Number of pages11
JournalFASEB journal : official publication of the Federation of American Societies for Experimental Biology
Volume33
Issue number8
DOIs
StatePublished - Aug 1 2019

Fingerprint

Gene transfer
Interleukin-23
Inbred NOD Mouse
Psoriatic Arthritis
Genes
T-cells
Interleukin-17
Skin
Activation analysis
Activation Analysis
T-Lymphocytes
Th17 Cells
Intervertebral Disc Degeneration
Synovitis
Charcoal
Cartilage
Articular Cartilage
Pathology
Erythema
Medical problems

Keywords

  • adenovirus
  • interleukin-17
  • interleukin-23
  • psoriasis
  • type 1 diabetes

ASJC Scopus subject areas

  • Biotechnology
  • Biochemistry
  • Molecular Biology
  • Genetics

Cite this

Adenoviral gene transfer of a single-chain IL-23 induces psoriatic arthritis-like symptoms in NOD mice. / Flores, Rafael R.; Carbo, Lana; Kim, Eun; Van Meter, Montina; De Padilla, Consuelo M.Lopez; Zhao, Jing; Colangelo, Debora; Yousefzadeh, Matthew J.; Angelini, Luise A.; Zhang, Lei; Pola, Enrico; Vo, Nam; Evans, Christopher H; Gambotto, Andrea; Niedernhofer, Laura J.; Robbins, Paul D.

In: FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Vol. 33, No. 8, 01.08.2019, p. 9505-9515.

Research output: Contribution to journalArticle

Flores, RR, Carbo, L, Kim, E, Van Meter, M, De Padilla, CML, Zhao, J, Colangelo, D, Yousefzadeh, MJ, Angelini, LA, Zhang, L, Pola, E, Vo, N, Evans, CH, Gambotto, A, Niedernhofer, LJ & Robbins, PD 2019, 'Adenoviral gene transfer of a single-chain IL-23 induces psoriatic arthritis-like symptoms in NOD mice', FASEB journal : official publication of the Federation of American Societies for Experimental Biology, vol. 33, no. 8, pp. 9505-9515. https://doi.org/10.1096/fj.201900420R
Flores, Rafael R. ; Carbo, Lana ; Kim, Eun ; Van Meter, Montina ; De Padilla, Consuelo M.Lopez ; Zhao, Jing ; Colangelo, Debora ; Yousefzadeh, Matthew J. ; Angelini, Luise A. ; Zhang, Lei ; Pola, Enrico ; Vo, Nam ; Evans, Christopher H ; Gambotto, Andrea ; Niedernhofer, Laura J. ; Robbins, Paul D. / Adenoviral gene transfer of a single-chain IL-23 induces psoriatic arthritis-like symptoms in NOD mice. In: FASEB journal : official publication of the Federation of American Societies for Experimental Biology. 2019 ; Vol. 33, No. 8. pp. 9505-9515.
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abstract = "Previously, we demonstrated that intratumoral delivery of adenoviral vector encoding single-chain (sc)IL-23 (Ad.scIL-23) was able to induce systemic antitumor immunity. Here, we examined the role of IL-23 in diabetes in nonobese diabetic mice. Intravenous delivery of Ad.scIL-23 did not accelerate the onset of hyperglycemia but instead resulted in the development of psoriatic arthritis. Ad.scIL-23-treated mice developed erythema, scales, and thickening of the skin, as well as intervertebral disc degeneration and extensive synovial hypertrophy and loss of articular cartilage in the knees. Immunological analysis revealed activation of conventional T helper type 17 cells and IL-17-producing γδ T cells along with a significant depletion and suppression of T cells in the pancreatic lymph nodes. Furthermore, treatment with anti-IL-17 antibody reduced joint and skin psoriatic arthritis pathologies. Thus, these Ad.scIL-23-treated mice represent a physiologically relevant model of psoriatic arthritis for understanding disease progression and for testing therapeutic approaches.-Flores, R. R., Carbo, L., Kim, E., Van Meter, M., De Padilla, C. M. L., Zhao, J., Colangelo, D., Yousefzadeh, M. J., Angelini, L. A., Zhang, L., Pola, E., Vo, N., Evans, C. H., Gambotto, A., Niedernhofer, L. J., Robbins, P. D. Adenoviral gene transfer of a single-chain IL-23 induces psoriatic arthritis-like symptoms in NOD mice.",
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T1 - Adenoviral gene transfer of a single-chain IL-23 induces psoriatic arthritis-like symptoms in NOD mice

AU - Flores, Rafael R.

AU - Carbo, Lana

AU - Kim, Eun

AU - Van Meter, Montina

AU - De Padilla, Consuelo M.Lopez

AU - Zhao, Jing

AU - Colangelo, Debora

AU - Yousefzadeh, Matthew J.

AU - Angelini, Luise A.

AU - Zhang, Lei

AU - Pola, Enrico

AU - Vo, Nam

AU - Evans, Christopher H

AU - Gambotto, Andrea

AU - Niedernhofer, Laura J.

AU - Robbins, Paul D.

PY - 2019/8/1

Y1 - 2019/8/1

N2 - Previously, we demonstrated that intratumoral delivery of adenoviral vector encoding single-chain (sc)IL-23 (Ad.scIL-23) was able to induce systemic antitumor immunity. Here, we examined the role of IL-23 in diabetes in nonobese diabetic mice. Intravenous delivery of Ad.scIL-23 did not accelerate the onset of hyperglycemia but instead resulted in the development of psoriatic arthritis. Ad.scIL-23-treated mice developed erythema, scales, and thickening of the skin, as well as intervertebral disc degeneration and extensive synovial hypertrophy and loss of articular cartilage in the knees. Immunological analysis revealed activation of conventional T helper type 17 cells and IL-17-producing γδ T cells along with a significant depletion and suppression of T cells in the pancreatic lymph nodes. Furthermore, treatment with anti-IL-17 antibody reduced joint and skin psoriatic arthritis pathologies. Thus, these Ad.scIL-23-treated mice represent a physiologically relevant model of psoriatic arthritis for understanding disease progression and for testing therapeutic approaches.-Flores, R. R., Carbo, L., Kim, E., Van Meter, M., De Padilla, C. M. L., Zhao, J., Colangelo, D., Yousefzadeh, M. J., Angelini, L. A., Zhang, L., Pola, E., Vo, N., Evans, C. H., Gambotto, A., Niedernhofer, L. J., Robbins, P. D. Adenoviral gene transfer of a single-chain IL-23 induces psoriatic arthritis-like symptoms in NOD mice.

AB - Previously, we demonstrated that intratumoral delivery of adenoviral vector encoding single-chain (sc)IL-23 (Ad.scIL-23) was able to induce systemic antitumor immunity. Here, we examined the role of IL-23 in diabetes in nonobese diabetic mice. Intravenous delivery of Ad.scIL-23 did not accelerate the onset of hyperglycemia but instead resulted in the development of psoriatic arthritis. Ad.scIL-23-treated mice developed erythema, scales, and thickening of the skin, as well as intervertebral disc degeneration and extensive synovial hypertrophy and loss of articular cartilage in the knees. Immunological analysis revealed activation of conventional T helper type 17 cells and IL-17-producing γδ T cells along with a significant depletion and suppression of T cells in the pancreatic lymph nodes. Furthermore, treatment with anti-IL-17 antibody reduced joint and skin psoriatic arthritis pathologies. Thus, these Ad.scIL-23-treated mice represent a physiologically relevant model of psoriatic arthritis for understanding disease progression and for testing therapeutic approaches.-Flores, R. R., Carbo, L., Kim, E., Van Meter, M., De Padilla, C. M. L., Zhao, J., Colangelo, D., Yousefzadeh, M. J., Angelini, L. A., Zhang, L., Pola, E., Vo, N., Evans, C. H., Gambotto, A., Niedernhofer, L. J., Robbins, P. D. Adenoviral gene transfer of a single-chain IL-23 induces psoriatic arthritis-like symptoms in NOD mice.

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