Adenosine analogs inhibit the guanine-7-methylation of mRNA cap structures

Keith A. Oxenrider, Guojun Bu, Thomas O. Sitz

Research output: Contribution to journalArticle

13 Scopus citations

Abstract

The adenosine analogs neplanocin A and deazaneplanocin A were observed to inhibit the in vivo guanine-7-methylation of mRNA cap structure using a new assay for hypomethylated RNA. Treatment of cultured mammalian cells with these adenosine analogs resulted in the same extent of hypomethylation of cap structure as did ethionine injection in mice. Neplanocin A and its non-metabolizable analog 3-deazaneplanocin A show the same maximal level of inhibition of methylation suggesting that these adenosine analogs exert their effects by elevating S-adenosylhomocysteine levels rather than by conversion to other inhibitory compounds.

Original languageEnglish (US)
Pages (from-to)273-277
Number of pages5
JournalFEBS Letters
Volume316
Issue number3
DOIs
StatePublished - Feb 1 1993

Keywords

  • 3-Deazaneplanocin A
  • Cap
  • Ethionine
  • Guanine-7-methyltransferase
  • Neplanocin A
  • mRNA methylation

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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