Adenoma-specific alterations of protein kinase C isozyme expression in Apc(MIN) mice

Irene K. Klein, Steven R. Ritland, Lawrence J. Burgart, Steven C. Ziesmer, Patrick C. Roche, Sandra J. Gendler, William E. Karnes

Research output: Contribution to journalArticle

23 Scopus citations

Abstract

Members of the protein kinase C (PKC) family appear to play important roles in colorectal carcinogenesis. To investigate the potential involvement of PKC isozymes in adenomatous transformation induced by inactivation of the adenomatous polyposis coli (APC) gene product, we examined protein levels and localizations of ten PKC isozymes by immunohistochemistry in normal and adenomatous ileal epithelium of Apc(MIN) mice. Compared with surrounding normal epithelium, adenomas showed dramatically reduced staining for PKCs α,β1, and ζ, as well as dysplasia-specific punctate nuclear staining of PKC μ. We conclude that reduced protein expression of PKC α, β1, and ζ, and nuclear localization of PKC μ are markers of, and are perhaps involved in, adenomatous transformation induced by APC inactivation in Apc(MIN) mice.

Original languageEnglish (US)
Pages (from-to)2077-2080
Number of pages4
JournalCancer research
Volume60
Issue number8
StatePublished - Apr 15 2000

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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    Klein, I. K., Ritland, S. R., Burgart, L. J., Ziesmer, S. C., Roche, P. C., Gendler, S. J., & Karnes, W. E. (2000). Adenoma-specific alterations of protein kinase C isozyme expression in Apc(MIN) mice. Cancer research, 60(8), 2077-2080.