TY - CHAP
T1 - Addressing Variability and Heterogeneity of Induced Pluripotent Stem Cell-Derived Cardiomyocytes
AU - Biendarra-Tiegs, Sherri M.
AU - Secreto, Frank J.
AU - Nelson, Timothy J.
N1 - Publisher Copyright:
© Springer Nature Switzerland AG 2019.
PY - 2020
Y1 - 2020
N2 - Induced pluripotent stem cells (iPSCs) offer great promise in the areas of disease modeling, basic research, drug development, and regenerative medicine. Much of their value comes from the fact that they can be used to create otherwise inaccessible cell types, such as cardiomyocytes, which are genetically matched to a patient or any other individual of interest. A consistent issue plaguing the iPSC platform, however, involves excessive variability exhibited in the differentiated products. This includes discrepancies in genetic, epigenetic, and transcriptional features, cell signalling, the cell types produced from cardiac differentiation, and cardiomyocyte functionality. These properties can result from both the somatic source cells and environmental conditions related to the derivation and handling of these cells. Understanding the potential sources of variability, along with determining which factors are most relevant to a given application, are essential in advancing iPSC-based technologies.
AB - Induced pluripotent stem cells (iPSCs) offer great promise in the areas of disease modeling, basic research, drug development, and regenerative medicine. Much of their value comes from the fact that they can be used to create otherwise inaccessible cell types, such as cardiomyocytes, which are genetically matched to a patient or any other individual of interest. A consistent issue plaguing the iPSC platform, however, involves excessive variability exhibited in the differentiated products. This includes discrepancies in genetic, epigenetic, and transcriptional features, cell signalling, the cell types produced from cardiac differentiation, and cardiomyocyte functionality. These properties can result from both the somatic source cells and environmental conditions related to the derivation and handling of these cells. Understanding the potential sources of variability, along with determining which factors are most relevant to a given application, are essential in advancing iPSC-based technologies.
KW - Cardiomyocytes
KW - Cellular microenvironment
KW - Differentiation
KW - Induced pluripotent stem cells
KW - Phenotypic variability
KW - Stem cell heterogeneity
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U2 - 10.1007/5584_2019_350
DO - 10.1007/5584_2019_350
M3 - Chapter
C2 - 30850960
AN - SCOPUS:85077734925
T3 - Advances in Experimental Medicine and Biology
SP - 1
EP - 29
BT - Advances in Experimental Medicine and Biology
PB - Springer
ER -