Addressing Variability and Heterogeneity of Induced Pluripotent Stem Cell-Derived Cardiomyocytes

Sherri M. Biendarra-Tiegs, Frank J. Secreto, Timothy J. Nelson

Research output: Chapter in Book/Report/Conference proceedingChapter

1 Scopus citations

Abstract

Induced pluripotent stem cells (iPSCs) offer great promise in the areas of disease modeling, basic research, drug development, and regenerative medicine. Much of their value comes from the fact that they can be used to create otherwise inaccessible cell types, such as cardiomyocytes, which are genetically matched to a patient or any other individual of interest. A consistent issue plaguing the iPSC platform, however, involves excessive variability exhibited in the differentiated products. This includes discrepancies in genetic, epigenetic, and transcriptional features, cell signalling, the cell types produced from cardiac differentiation, and cardiomyocyte functionality. These properties can result from both the somatic source cells and environmental conditions related to the derivation and handling of these cells. Understanding the potential sources of variability, along with determining which factors are most relevant to a given application, are essential in advancing iPSC-based technologies.

Original languageEnglish (US)
Title of host publicationAdvances in Experimental Medicine and Biology
PublisherSpringer
Pages1-29
Number of pages29
DOIs
StatePublished - Jan 1 2020

Publication series

NameAdvances in Experimental Medicine and Biology
Volume1212
ISSN (Print)0065-2598
ISSN (Electronic)2214-8019

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Keywords

  • Cardiomyocytes
  • Cellular microenvironment
  • Differentiation
  • Induced pluripotent stem cells
  • Phenotypic variability
  • Stem cell heterogeneity

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Biendarra-Tiegs, S. M., Secreto, F. J., & Nelson, T. J. (2020). Addressing Variability and Heterogeneity of Induced Pluripotent Stem Cell-Derived Cardiomyocytes. In Advances in Experimental Medicine and Biology (pp. 1-29). (Advances in Experimental Medicine and Biology; Vol. 1212). Springer. https://doi.org/10.1007/5584_2019_350