Adaptive changes in pancreatic beta cell fractional area and beta cell turnover in human pregnancy

A. E. Butler, L. Cao-Minh, Galasso R. Galasso, R. A. Rizza, A. Corradin, C. Cobelli, P. C. Butler

Research output: Contribution to journalArticle

211 Citations (Scopus)

Abstract

Aims/hypothesis We sought to establish the extent and basis for adaptive changes in beta cell numbers in human pregnancy. Methods Pancreas was obtained at autopsy from women who had died while pregnant (n=18), post-partum (n=6) or were not pregnant at or shortly before death (controls; n= 20). Pancreases were evaluated for fractional pancreatic beta cell area, islet size and islet fraction of beta cells, beta cell replication (Ki67) and apoptosis (TUNEL), and indirect markers of beta cell neogenesis (insulin-positive cells in ducts and scattered beta cells in pancreas). Results The pancreatic fractional beta cell area was increased by ∼ 1.4-fold in human pregnancy, with no change in mean beta cell size. In pregnancy there were more small islets rather than an increase in islet size or beta cells per islet. No increase in beta cell replication or change in beta cell apoptosis was detected, but duct cells positive for insulin and scattered beta cells were increased with pregnancy.Conclusions/interpretation The adaptive increase in beta cell numbers in human pregnancy is not as great as in most reports in rodents. This increase in humans is achieved by increased numbers of beta cells in apparently new small islets, rather than duplication of beta cells in existing islets, which is characteristic of pregnancy in rodents.

Original languageEnglish (US)
Pages (from-to)2167-2176
Number of pages10
JournalDiabetologia
Volume53
Issue number10
DOIs
StatePublished - Oct 2010

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Insulin-Secreting Cells
Pregnancy
Pancreas
Cell Count
Rodentia
Insulin
Apoptosis
In Situ Nick-End Labeling
Islets of Langerhans
Cell Size
Autopsy

Keywords

  • Human
  • Islet
  • Pancreas
  • Pregnancy

ASJC Scopus subject areas

  • Internal Medicine
  • Endocrinology, Diabetes and Metabolism

Cite this

Butler, A. E., Cao-Minh, L., R. Galasso, G., Rizza, R. A., Corradin, A., Cobelli, C., & Butler, P. C. (2010). Adaptive changes in pancreatic beta cell fractional area and beta cell turnover in human pregnancy. Diabetologia, 53(10), 2167-2176. https://doi.org/10.1007/s00125-010-1809-6

Adaptive changes in pancreatic beta cell fractional area and beta cell turnover in human pregnancy. / Butler, A. E.; Cao-Minh, L.; R. Galasso, Galasso; Rizza, R. A.; Corradin, A.; Cobelli, C.; Butler, P. C.

In: Diabetologia, Vol. 53, No. 10, 10.2010, p. 2167-2176.

Research output: Contribution to journalArticle

Butler, AE, Cao-Minh, L, R. Galasso, G, Rizza, RA, Corradin, A, Cobelli, C & Butler, PC 2010, 'Adaptive changes in pancreatic beta cell fractional area and beta cell turnover in human pregnancy', Diabetologia, vol. 53, no. 10, pp. 2167-2176. https://doi.org/10.1007/s00125-010-1809-6
Butler AE, Cao-Minh L, R. Galasso G, Rizza RA, Corradin A, Cobelli C et al. Adaptive changes in pancreatic beta cell fractional area and beta cell turnover in human pregnancy. Diabetologia. 2010 Oct;53(10):2167-2176. https://doi.org/10.1007/s00125-010-1809-6
Butler, A. E. ; Cao-Minh, L. ; R. Galasso, Galasso ; Rizza, R. A. ; Corradin, A. ; Cobelli, C. ; Butler, P. C. / Adaptive changes in pancreatic beta cell fractional area and beta cell turnover in human pregnancy. In: Diabetologia. 2010 ; Vol. 53, No. 10. pp. 2167-2176.
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