Adaptation of the 51cr cytotoxic assay for rapid H-2 classifications on peripheral blood cells

C. S. David, D. C. Shreffler

Research output: Contribution to journalArticle

16 Scopus citations

Abstract

A new method for rapid, routine classifications of H-2 type for genetic crosses or wild populations is described. It is based upon absorption in the standard 51cr cytotoxic assay, with small quantities of washed peripheral blood cells (erythrocytes and leukocytes), which may be obtained without killing the test animal. After initial absorption, the blood cells are sedimented and the remainder of the test is completed, without removal of the absorption supernatant, thus substantially enhancing the speed and efficiency of the test. Data are presented to demonstrate that the method is comparable, if not superior, to the standard hemagglutination technique.

Original languageEnglish (US)
Pages (from-to)414-420
Number of pages7
JournalTransplantation
Volume13
Issue number4
DOIs
StatePublished - Apr 1972

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ASJC Scopus subject areas

  • Transplantation

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