Adaptação de um sistema de ensaio biológico para detecção de fatores relaxantes endoteliais derivados do endocárdio atrial canino

Translated title of the contribution: Adaptation of bioassay to detect endotheliumderived relaxing factors from the canine atrial endocardium

Yeow Leng Chua, Paulo Roberto Barbosa Evora, Andrea Carla Celotto, Paul Joseph Pearson, Berent Discigil, Hartzell Vernon Schaff

Research output: Contribution to journalArticle

1 Scopus citations

Abstract

Objective: The aim of this study was to assess the release of endothelium-derived relaxing factors from the endocardium of canine atrial appendage. Method: To study the release of endothelium-derived relaxing factor (EDRF) from intact atrial endocardial endothelium, tube-shaped sutures of canine atrial appendages were performed and effluents from these tubes were bioassayed (isolated perfused organ chamber system) for detection of EDRF in canine coronary artery. Results: Effluent from the right atrial appendage caused a relaxation of 58.4 + 10.1% and the left atrial appendage 74.9 + 8.5% from the initial prostagladin FF2α contraction in coronary artery. No significant statistical difference was detected in effluent from the right and left atrial appendages. This relaxation was abolished by treating the heart tubes with Triton X-100 and reduced by treatment with LNMMA, a competitive inhibitor of nitric oxide and with indomethacin, an inhibitor of the cyclo-oxygenase pathway, also indicating the release of vasodilatory prostanoids from the endocardial endothelium. Conclusion: This study showed for the first time, in vitro luminal release of EDRF and prostacyclin from the canine heart atrium. The ability of the endocardial endothelium to produce these factors could play an important role in preventing thrombus formation in the cardiac chambers.

Translated title of the contributionAdaptation of bioassay to detect endotheliumderived relaxing factors from the canine atrial endocardium
Original languagePortuguese
Pages (from-to)225-232
Number of pages8
JournalBrazilian Journal of Cardiovascular Surgery
Volume24
Issue number2
DOIs
StatePublished - 2009

Keywords

  • Endocardium
  • Endothelium
  • Heart atria
  • Nitric oxide

ASJC Scopus subject areas

  • Surgery
  • Cardiology and Cardiovascular Medicine

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