Acute female hypogonadism alters adipose tissue fatty acid storage factors and chylomicronemia

Sylvia Santosa, Sara L. Bonnes, Michael Dennis Jensen

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Context: Chronic sex steroid deficiency has effects on adipose fatty acid (FA) storage mechanisms and fat oxidation, but the chronology of events are not well understood. Objective: The objective of the study was to examine the acute effects of female sex steroid suppression on cellular mechanisms affecting abdominal and femoral subcutaneous adipose tissue FA storage. Design: This study had a randomized, longitudinal, parallel study design. Setting: The study was conducted at the Mayo Clinic Clinical Research Unit. Participants: Thirty-eight nonsmoking premenopausal women aged 18-50 years participated in the study. Intervention: The intervention included randomization to receive one of the following: 1) no treatment (control), 2) 3.75 mg of Lupron, or 3) 3.75 mg of Lupron and estrogen, but not progesterone, replacement for 49 days, resulting in at least 4 weeks of sex steroid suppression. Main Outcome Measures: Body composition, fat cell size, postprandial chylomicron and nonchylomicron triglyceride concentrations, adipose tissue meal FA storage, direct free fatty acid storage, lipoprotein lipase, acyl CoA synthetase, and diacylglycerol acyltransferase activities, and CD36 content were measured. Results: Compared with the control group, the fed state femoral lipoprotein lipase activity was reduced in women taking Lupron and those taking Lupron and estrogen replacement. In addition, we observed significantly greater postprandial chylomicronemia in the Lupron group than in the other two groups. There were no differences in overall fat storage and oxidation. Depending on the mode of data expression (per unit lipid vs per 1000 adipocytes), there were modest changes in acyl CoA synthetase, diacylglycerol acyltransferase, and CD36 in response to acute sex hormone suppression. Conclusions: Our results suggest estrogen and progesterone may have different effects on the regulation of FA metabolism and that acute sex steroid deficiency in women does not alter fat storage and oxidation.

Original languageEnglish (US)
Pages (from-to)2089-2098
Number of pages10
JournalJournal of Clinical Endocrinology and Metabolism
Volume101
Issue number5
DOIs
StatePublished - May 1 2016

Fingerprint

Leuprolide
Hypogonadism
Adipose Tissue
Fatty Acids
Tissue
Diacylglycerol O-Acyltransferase
Fats
Steroids
Coenzyme A Ligases
Estrogens
Lipoprotein Lipase
Thigh
Adipocytes
Oxidation
Progesterone
Abdominal Subcutaneous Fat
Chronology
Chylomicrons
Estrogen Replacement Therapy
Gonadal Steroid Hormones

ASJC Scopus subject areas

  • Biochemistry
  • Clinical Biochemistry
  • Endocrinology
  • Biochemistry, medical
  • Endocrinology, Diabetes and Metabolism

Cite this

Acute female hypogonadism alters adipose tissue fatty acid storage factors and chylomicronemia. / Santosa, Sylvia; Bonnes, Sara L.; Jensen, Michael Dennis.

In: Journal of Clinical Endocrinology and Metabolism, Vol. 101, No. 5, 01.05.2016, p. 2089-2098.

Research output: Contribution to journalArticle

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abstract = "Context: Chronic sex steroid deficiency has effects on adipose fatty acid (FA) storage mechanisms and fat oxidation, but the chronology of events are not well understood. Objective: The objective of the study was to examine the acute effects of female sex steroid suppression on cellular mechanisms affecting abdominal and femoral subcutaneous adipose tissue FA storage. Design: This study had a randomized, longitudinal, parallel study design. Setting: The study was conducted at the Mayo Clinic Clinical Research Unit. Participants: Thirty-eight nonsmoking premenopausal women aged 18-50 years participated in the study. Intervention: The intervention included randomization to receive one of the following: 1) no treatment (control), 2) 3.75 mg of Lupron, or 3) 3.75 mg of Lupron and estrogen, but not progesterone, replacement for 49 days, resulting in at least 4 weeks of sex steroid suppression. Main Outcome Measures: Body composition, fat cell size, postprandial chylomicron and nonchylomicron triglyceride concentrations, adipose tissue meal FA storage, direct free fatty acid storage, lipoprotein lipase, acyl CoA synthetase, and diacylglycerol acyltransferase activities, and CD36 content were measured. Results: Compared with the control group, the fed state femoral lipoprotein lipase activity was reduced in women taking Lupron and those taking Lupron and estrogen replacement. In addition, we observed significantly greater postprandial chylomicronemia in the Lupron group than in the other two groups. There were no differences in overall fat storage and oxidation. Depending on the mode of data expression (per unit lipid vs per 1000 adipocytes), there were modest changes in acyl CoA synthetase, diacylglycerol acyltransferase, and CD36 in response to acute sex hormone suppression. Conclusions: Our results suggest estrogen and progesterone may have different effects on the regulation of FA metabolism and that acute sex steroid deficiency in women does not alter fat storage and oxidation.",
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