Acute effects of testosterone infusion on the serum luteinizing hormone profile in eumenorrheic and polycystic ovary syndrome adolescents

María Gabriela Ropelato, María Cecilia García Rudaz, María Eugenia Escobar, Sonia Viviana Bengolea, María Luján Calcagno, Johannes D Veldhuis, Marta Barontini

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Context: Little is known about the neuroendocrine effects of androgens on the GnRH-LH unit in females. Objective: Our objective was to evaluate androgen negative feedback on the GnRH-LH axis in eumenorrheic and polycystic ovary syndrome (PCOS) adolescents. Design and Setting:Weconducted a prospective, longitudinal, randomized, double-blind study at a pediatric endocrinology clinical research center. Participants: Seven nonobese PCOS adolescents and seven matched controls (C) were studied in the early follicular phase of three consecutive menstrual cycles or in three consecutive months. Intervention: Pulsatile LH release was determined during saline [baseline (B)] and constant testosterone (T) infusions: low dose (T-LD) 0.75 and high dose (T-HD) 2.5 mg/12 h iv. Blood samples were drawn every 20 min overnight. Main Outcome Measures: LH (immunofluorometric assay) and T (electrochemiluminescence immunoassay) were determined at B, and during both T-LD and T-HD. LH profiles were analyzed by deconvolution and approximate entropy analyses. Results: On T-LD, C and PCOS serum T levels increased 2- to 3-fold vs. B. On T-HD, T values doubled in both groups vs. T-LD. Controls on T-LD had greater 12-h pulsatile LH secretion rate (P < 0.05 vs. B) and on T-HD had lower mean, pulsatile, basal LH release and LH approximate entropy (vs. B, P< 0.05). PCOS did not respond to T-LD. High-dose T did not alter mean LH in PCOS but increased pulsatile and reduced basal LH secretion. Conclusions: PCOS adolescents have impaired suppression of pulsatile LH secretion rate consistent with reduced androgen negative feedback. Attenuation of T feedback in nonobese adolescents with PCOS extends the pathophysiology of this syndrome.

Original languageEnglish (US)
Pages (from-to)3602-3610
Number of pages9
JournalJournal of Clinical Endocrinology and Metabolism
Volume94
Issue number9
DOIs
StatePublished - Sep 2009

Fingerprint

Polycystic Ovary Syndrome
Luteinizing Hormone
Androgens
Testosterone
Feedback
Gonadotropin-Releasing Hormone
Endocrinology
Entropy
Serum
Pediatrics
Deconvolution
Assays
Blood
Fluoroimmunoassay
Follicular Phase
Theophylline
Menstrual Cycle
Immunoassay
Double-Blind Method
Outcome Assessment (Health Care)

ASJC Scopus subject areas

  • Biochemistry
  • Clinical Biochemistry
  • Endocrinology
  • Biochemistry, medical
  • Endocrinology, Diabetes and Metabolism

Cite this

Acute effects of testosterone infusion on the serum luteinizing hormone profile in eumenorrheic and polycystic ovary syndrome adolescents. / Ropelato, María Gabriela; Rudaz, María Cecilia García; Escobar, María Eugenia; Bengolea, Sonia Viviana; Calcagno, María Luján; Veldhuis, Johannes D; Barontini, Marta.

In: Journal of Clinical Endocrinology and Metabolism, Vol. 94, No. 9, 09.2009, p. 3602-3610.

Research output: Contribution to journalArticle

Ropelato, María Gabriela ; Rudaz, María Cecilia García ; Escobar, María Eugenia ; Bengolea, Sonia Viviana ; Calcagno, María Luján ; Veldhuis, Johannes D ; Barontini, Marta. / Acute effects of testosterone infusion on the serum luteinizing hormone profile in eumenorrheic and polycystic ovary syndrome adolescents. In: Journal of Clinical Endocrinology and Metabolism. 2009 ; Vol. 94, No. 9. pp. 3602-3610.
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abstract = "Context: Little is known about the neuroendocrine effects of androgens on the GnRH-LH unit in females. Objective: Our objective was to evaluate androgen negative feedback on the GnRH-LH axis in eumenorrheic and polycystic ovary syndrome (PCOS) adolescents. Design and Setting:Weconducted a prospective, longitudinal, randomized, double-blind study at a pediatric endocrinology clinical research center. Participants: Seven nonobese PCOS adolescents and seven matched controls (C) were studied in the early follicular phase of three consecutive menstrual cycles or in three consecutive months. Intervention: Pulsatile LH release was determined during saline [baseline (B)] and constant testosterone (T) infusions: low dose (T-LD) 0.75 and high dose (T-HD) 2.5 mg/12 h iv. Blood samples were drawn every 20 min overnight. Main Outcome Measures: LH (immunofluorometric assay) and T (electrochemiluminescence immunoassay) were determined at B, and during both T-LD and T-HD. LH profiles were analyzed by deconvolution and approximate entropy analyses. Results: On T-LD, C and PCOS serum T levels increased 2- to 3-fold vs. B. On T-HD, T values doubled in both groups vs. T-LD. Controls on T-LD had greater 12-h pulsatile LH secretion rate (P < 0.05 vs. B) and on T-HD had lower mean, pulsatile, basal LH release and LH approximate entropy (vs. B, P< 0.05). PCOS did not respond to T-LD. High-dose T did not alter mean LH in PCOS but increased pulsatile and reduced basal LH secretion. Conclusions: PCOS adolescents have impaired suppression of pulsatile LH secretion rate consistent with reduced androgen negative feedback. Attenuation of T feedback in nonobese adolescents with PCOS extends the pathophysiology of this syndrome.",
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T1 - Acute effects of testosterone infusion on the serum luteinizing hormone profile in eumenorrheic and polycystic ovary syndrome adolescents

AU - Ropelato, María Gabriela

AU - Rudaz, María Cecilia García

AU - Escobar, María Eugenia

AU - Bengolea, Sonia Viviana

AU - Calcagno, María Luján

AU - Veldhuis, Johannes D

AU - Barontini, Marta

PY - 2009/9

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N2 - Context: Little is known about the neuroendocrine effects of androgens on the GnRH-LH unit in females. Objective: Our objective was to evaluate androgen negative feedback on the GnRH-LH axis in eumenorrheic and polycystic ovary syndrome (PCOS) adolescents. Design and Setting:Weconducted a prospective, longitudinal, randomized, double-blind study at a pediatric endocrinology clinical research center. Participants: Seven nonobese PCOS adolescents and seven matched controls (C) were studied in the early follicular phase of three consecutive menstrual cycles or in three consecutive months. Intervention: Pulsatile LH release was determined during saline [baseline (B)] and constant testosterone (T) infusions: low dose (T-LD) 0.75 and high dose (T-HD) 2.5 mg/12 h iv. Blood samples were drawn every 20 min overnight. Main Outcome Measures: LH (immunofluorometric assay) and T (electrochemiluminescence immunoassay) were determined at B, and during both T-LD and T-HD. LH profiles were analyzed by deconvolution and approximate entropy analyses. Results: On T-LD, C and PCOS serum T levels increased 2- to 3-fold vs. B. On T-HD, T values doubled in both groups vs. T-LD. Controls on T-LD had greater 12-h pulsatile LH secretion rate (P < 0.05 vs. B) and on T-HD had lower mean, pulsatile, basal LH release and LH approximate entropy (vs. B, P< 0.05). PCOS did not respond to T-LD. High-dose T did not alter mean LH in PCOS but increased pulsatile and reduced basal LH secretion. Conclusions: PCOS adolescents have impaired suppression of pulsatile LH secretion rate consistent with reduced androgen negative feedback. Attenuation of T feedback in nonobese adolescents with PCOS extends the pathophysiology of this syndrome.

AB - Context: Little is known about the neuroendocrine effects of androgens on the GnRH-LH unit in females. Objective: Our objective was to evaluate androgen negative feedback on the GnRH-LH axis in eumenorrheic and polycystic ovary syndrome (PCOS) adolescents. Design and Setting:Weconducted a prospective, longitudinal, randomized, double-blind study at a pediatric endocrinology clinical research center. Participants: Seven nonobese PCOS adolescents and seven matched controls (C) were studied in the early follicular phase of three consecutive menstrual cycles or in three consecutive months. Intervention: Pulsatile LH release was determined during saline [baseline (B)] and constant testosterone (T) infusions: low dose (T-LD) 0.75 and high dose (T-HD) 2.5 mg/12 h iv. Blood samples were drawn every 20 min overnight. Main Outcome Measures: LH (immunofluorometric assay) and T (electrochemiluminescence immunoassay) were determined at B, and during both T-LD and T-HD. LH profiles were analyzed by deconvolution and approximate entropy analyses. Results: On T-LD, C and PCOS serum T levels increased 2- to 3-fold vs. B. On T-HD, T values doubled in both groups vs. T-LD. Controls on T-LD had greater 12-h pulsatile LH secretion rate (P < 0.05 vs. B) and on T-HD had lower mean, pulsatile, basal LH release and LH approximate entropy (vs. B, P< 0.05). PCOS did not respond to T-LD. High-dose T did not alter mean LH in PCOS but increased pulsatile and reduced basal LH secretion. Conclusions: PCOS adolescents have impaired suppression of pulsatile LH secretion rate consistent with reduced androgen negative feedback. Attenuation of T feedback in nonobese adolescents with PCOS extends the pathophysiology of this syndrome.

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