Activity of electrical current in experimental Propionibacterium acnes foreign-body osteomyelitis

Suzannah M. Schmidt-Malan, Cassandra L. Brinkman, Kerryl E. Greenwood-Quaintance, Melissa J. Karau, Jayawant Mandrekar, Robin Patel

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Foreign-body-associated infections are often difficult to treat, given that the associated microorganisms are in a biofilm state. Previously, we showed that a low-amperage direct electrical current (DC) reduces Propionibacterium acnes biofilms formed on implant-associated materials in vitro. In this study, low-amperage DC was compared to ceftriaxone treatment or no treatment in a novel rat femur model of foreign-body osteomyelitis. A platinum implant seeded with a P. acnes biofilm (107 CFU/cm2) and 109 CFU of planktonic P. acnes was placed in the femoral medullary cavity. One week later, rats were assigned to one of three treatment groups: no treatment, ceftriaxone treatment, or 200-μA-DC treatment. After 2 weeks of treatment, there were fewer bacteria in the bones of the ceftriaxone group (3.06 log10 CFU/g of bone [P = 0.0209]) and the 200-μA-DC group (0.5 log10 CFU/g [P = 0.0015]) than in those of the control group (6.58 log10 CFU/g). The DC-exposed animals exhibited fewer bacteria than the ceftriaxone-treated animals (P = 0.0330). There were fewer bacteria on the implanted wires in the groups treated with ceftriaxone (0.1 log10 CFU/cm2) or a 200-μA DC (0.1 log10 CFU/cm2) than in the control group (2.53 log10 CFU/cm2 [P, 0.0003 for both comparisons]). Low-amperage DC may be useful for treating, or aiding in the treatment of, foreign-body infections caused by P. acnes.

Original languageEnglish (US)
Article numbere01863
JournalAntimicrobial Agents and Chemotherapy
Volume61
Issue number2
DOIs
StatePublished - Feb 1 2017

Fingerprint

Propionibacterium acnes
Ceftriaxone
Osteomyelitis
Foreign Bodies
Biofilms
Bacteria
Bone and Bones
Control Groups
Thigh
Infection
Platinum
Femur

Keywords

  • Direct electrical current
  • Osteomyelitis
  • Propionibacterium acnes

ASJC Scopus subject areas

  • Pharmacology
  • Pharmacology (medical)
  • Infectious Diseases

Cite this

Activity of electrical current in experimental Propionibacterium acnes foreign-body osteomyelitis. / Schmidt-Malan, Suzannah M.; Brinkman, Cassandra L.; Greenwood-Quaintance, Kerryl E.; Karau, Melissa J.; Mandrekar, Jayawant; Patel, Robin.

In: Antimicrobial Agents and Chemotherapy, Vol. 61, No. 2, e01863, 01.02.2017.

Research output: Contribution to journalArticle

Schmidt-Malan, Suzannah M. ; Brinkman, Cassandra L. ; Greenwood-Quaintance, Kerryl E. ; Karau, Melissa J. ; Mandrekar, Jayawant ; Patel, Robin. / Activity of electrical current in experimental Propionibacterium acnes foreign-body osteomyelitis. In: Antimicrobial Agents and Chemotherapy. 2017 ; Vol. 61, No. 2.
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abstract = "Foreign-body-associated infections are often difficult to treat, given that the associated microorganisms are in a biofilm state. Previously, we showed that a low-amperage direct electrical current (DC) reduces Propionibacterium acnes biofilms formed on implant-associated materials in vitro. In this study, low-amperage DC was compared to ceftriaxone treatment or no treatment in a novel rat femur model of foreign-body osteomyelitis. A platinum implant seeded with a P. acnes biofilm (107 CFU/cm2) and 109 CFU of planktonic P. acnes was placed in the femoral medullary cavity. One week later, rats were assigned to one of three treatment groups: no treatment, ceftriaxone treatment, or 200-μA-DC treatment. After 2 weeks of treatment, there were fewer bacteria in the bones of the ceftriaxone group (3.06 log10 CFU/g of bone [P = 0.0209]) and the 200-μA-DC group (0.5 log10 CFU/g [P = 0.0015]) than in those of the control group (6.58 log10 CFU/g). The DC-exposed animals exhibited fewer bacteria than the ceftriaxone-treated animals (P = 0.0330). There were fewer bacteria on the implanted wires in the groups treated with ceftriaxone (0.1 log10 CFU/cm2) or a 200-μA DC (0.1 log10 CFU/cm2) than in the control group (2.53 log10 CFU/cm2 [P, 0.0003 for both comparisons]). Low-amperage DC may be useful for treating, or aiding in the treatment of, foreign-body infections caused by P. acnes.",
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