Activation of transcriptional activities of AP1 and SRE by a novel zinc finger protein ZNF445

Kuntian Luo, Jian Yuan, Yuxi Shan, Jie Li, Meng Xu, Yanhui Cui, Wenwen Tang, Bo Wan, Nailing Zhang, Yimin Wu, Long Yu

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Zinc finger proteins play important roles in various cellular functions, including cell proliferation, differentiation, and apoptosis. Mitogen-activated protein kinase (MAPK) signal transduction pathways are one of the most common mechanisms in eukaryotic cell regulation. Many transcription factors are important targets of MAPKs. In this study, we identified a novel gene encoding a zinc finger protein named ZNF445. The ZNF445 mRNA consists of 9105 nucleotides and has a 1031-amino acid open reading frame. The predicted 119-kDa protein contains a leucine-rich region (LER or SCAN domain) at the N-terminus, followed by a well-conserved Krüppel-associated box (KRAB) domain. At the C-terminus of the protein, there are 14 C2H2 (Cys2-His2) zinc finger motifs. ZNF445 gene is mapped to chromosome 3p21.32. Northern blot analysis indicates that a 9.1 kb transcript specific for ZNF445 is expressed in uterus, thymus, small intestine, colon, pancreas, peripheral blood leukocyte, and especially at a higher level in the testis and skeletal muscle in human adult tissues. ZNF445 protein was located in the nucleus when overexpressed in cultured cells. Reporter gene assays showed that ZNF445 is a transcriptional repressor, and overexpression of ZNF445 in the HEK 293T cells activates the transcriptional activities of AP1 and SRE. Deletion studies showed that the SCAN domain of ZNF445 may be involved in this activation. Furthermore, we found that expression of ZNF445 can increase p42/44 MAPK, MEK and Raf-1 phosphorylation. These results clearly indicate that ZNF445 is a member of the zinc finger transcription factor family and may function in MAPK pathway through Raf-1/MEK/p42/44 MAPK signals.

Original languageEnglish (US)
Pages (from-to)89-100
Number of pages12
JournalGene
Volume367
Issue number1-2
DOIs
StatePublished - Feb 15 2006
Externally publishedYes

Fingerprint

Zinc Fingers
Transcriptional Activation
Mitogen-Activated Protein Kinase 1
Mitogen-Activated Protein Kinase Kinases
Mitogen-Activated Protein Kinases
Proteins
Transcription Factors
Proto-Oncogene Proteins c-raf
HEK293 Cells
Eukaryotic Cells
Protein C
Reporter Genes
Leucine
Northern Blotting
Thymus Gland
Open Reading Frames
Genes
Small Intestine
Uterus
Testis

Keywords

  • CH zinc finger
  • MAPK signaling pathway
  • SCAN (LER) domain
  • Transcription factor

ASJC Scopus subject areas

  • Genetics

Cite this

Activation of transcriptional activities of AP1 and SRE by a novel zinc finger protein ZNF445. / Luo, Kuntian; Yuan, Jian; Shan, Yuxi; Li, Jie; Xu, Meng; Cui, Yanhui; Tang, Wenwen; Wan, Bo; Zhang, Nailing; Wu, Yimin; Yu, Long.

In: Gene, Vol. 367, No. 1-2, 15.02.2006, p. 89-100.

Research output: Contribution to journalArticle

Luo, K, Yuan, J, Shan, Y, Li, J, Xu, M, Cui, Y, Tang, W, Wan, B, Zhang, N, Wu, Y & Yu, L 2006, 'Activation of transcriptional activities of AP1 and SRE by a novel zinc finger protein ZNF445', Gene, vol. 367, no. 1-2, pp. 89-100. https://doi.org/10.1016/j.gene.2005.09.023
Luo, Kuntian ; Yuan, Jian ; Shan, Yuxi ; Li, Jie ; Xu, Meng ; Cui, Yanhui ; Tang, Wenwen ; Wan, Bo ; Zhang, Nailing ; Wu, Yimin ; Yu, Long. / Activation of transcriptional activities of AP1 and SRE by a novel zinc finger protein ZNF445. In: Gene. 2006 ; Vol. 367, No. 1-2. pp. 89-100.
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AU - Xu, Meng

AU - Cui, Yanhui

AU - Tang, Wenwen

AU - Wan, Bo

AU - Zhang, Nailing

AU - Wu, Yimin

AU - Yu, Long

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