Activation of the bone-related Runx2/Cbfa1 promoter in mesenchymal condensations and developing chondrocytes of the axial skeleton

Christopher J. Lengner; Hicham Drissi; Je Yong Choi; Andre J. Van Wijnen; Janet L. Stein; Gary S. Stein; Jane B. Lian

doi:10.1016/S0925-4773(02)00050-3

Activation of the bone-related Runx2/Cbfa1 promoter in mesenchymal condensations and developing chondrocytes of the axial skeleton

Christopher J. Lengner, Hicham Drissi, Je Yong Choi, Andre J. Van Wijnen, Janet L. Stein, Gary S. Stein, Jane B. Lian

Orthopedic Surgery

Research output: Contribution to journal › Article › peer-review

56 Scopus citations

Abstract

The Runx2/Cbfa1 transcription factor regulates a program of gene expression necessary for skeletal development. To understand signals mediating skeletal formation, we examined the in vivo spatio-temporal activity of the Runx2 P1 promoter which controls expression of the bone-related Type II isoform. Transgenic mice carrying 3 kb of Runx2 promoter fused to the lacZ gene exhibit localized promoter activity in early mesenchymal condensations shortly after the embryonic turning event. Expression in developing mesenchyme continues throughout chondrogenesis and is restricted to the axial skeleton. Our data support a function for Runx2 in establishment of the prechondrocytic skeletal primordium.

Original language	English (US)
Pages (from-to)	167-170
Number of pages	4
Journal	Mechanisms of Development
Volume	114
Issue number	1-2
DOIs	https://doi.org/10.1016/S0925-4773(02)00050-3
State	Published - 2002

Keywords

AML3
Cartilage
Development
Runt homology domain
Sclerotome
Somite
Transcription
Transgene
β-Galactosidase

ASJC Scopus subject areas

Embryology
Developmental Biology

Access to Document

10.1016/S0925-4773(02)00050-3

Cite this

@article{22aff7547673444b9bf4eb0f559cb846,

title = "Activation of the bone-related Runx2/Cbfa1 promoter in mesenchymal condensations and developing chondrocytes of the axial skeleton",

abstract = "The Runx2/Cbfa1 transcription factor regulates a program of gene expression necessary for skeletal development. To understand signals mediating skeletal formation, we examined the in vivo spatio-temporal activity of the Runx2 P1 promoter which controls expression of the bone-related Type II isoform. Transgenic mice carrying 3 kb of Runx2 promoter fused to the lacZ gene exhibit localized promoter activity in early mesenchymal condensations shortly after the embryonic turning event. Expression in developing mesenchyme continues throughout chondrogenesis and is restricted to the axial skeleton. Our data support a function for Runx2 in establishment of the prechondrocytic skeletal primordium.",

keywords = "AML3, Cartilage, Development, Runt homology domain, Sclerotome, Somite, Transcription, Transgene, β-Galactosidase",

author = "Lengner, {Christopher J.} and Hicham Drissi and Choi, {Je Yong} and {Van Wijnen}, {Andre J.} and Stein, {Janet L.} and Stein, {Gary S.} and Lian, {Jane B.}",

note = "Funding Information: This work was supported by grants AR39588 and DE12528 from the National Institutes of Health. The contents of this paper are solely the responsibility of the authors and do not necessarily represent the official views of the National Institutes of Health. We thank Heather Steinman and Stephen Jones at the University of Massachusetts for helpful discussions during these studies.",

year = "2002",

doi = "10.1016/S0925-4773(02)00050-3",

language = "English (US)",

volume = "114",

pages = "167--170",

journal = "Mechanisms of Development",

issn = "0925-4773",

publisher = "Elsevier Ireland Ltd",

number = "1-2",

}

TY - JOUR

T1 - Activation of the bone-related Runx2/Cbfa1 promoter in mesenchymal condensations and developing chondrocytes of the axial skeleton

AU - Lengner, Christopher J.

AU - Drissi, Hicham

AU - Choi, Je Yong

AU - Van Wijnen, Andre J.

AU - Stein, Janet L.

AU - Stein, Gary S.

AU - Lian, Jane B.

N1 - Funding Information: This work was supported by grants AR39588 and DE12528 from the National Institutes of Health. The contents of this paper are solely the responsibility of the authors and do not necessarily represent the official views of the National Institutes of Health. We thank Heather Steinman and Stephen Jones at the University of Massachusetts for helpful discussions during these studies.

PY - 2002

Y1 - 2002

N2 - The Runx2/Cbfa1 transcription factor regulates a program of gene expression necessary for skeletal development. To understand signals mediating skeletal formation, we examined the in vivo spatio-temporal activity of the Runx2 P1 promoter which controls expression of the bone-related Type II isoform. Transgenic mice carrying 3 kb of Runx2 promoter fused to the lacZ gene exhibit localized promoter activity in early mesenchymal condensations shortly after the embryonic turning event. Expression in developing mesenchyme continues throughout chondrogenesis and is restricted to the axial skeleton. Our data support a function for Runx2 in establishment of the prechondrocytic skeletal primordium.

AB - The Runx2/Cbfa1 transcription factor regulates a program of gene expression necessary for skeletal development. To understand signals mediating skeletal formation, we examined the in vivo spatio-temporal activity of the Runx2 P1 promoter which controls expression of the bone-related Type II isoform. Transgenic mice carrying 3 kb of Runx2 promoter fused to the lacZ gene exhibit localized promoter activity in early mesenchymal condensations shortly after the embryonic turning event. Expression in developing mesenchyme continues throughout chondrogenesis and is restricted to the axial skeleton. Our data support a function for Runx2 in establishment of the prechondrocytic skeletal primordium.

KW - AML3

KW - Cartilage

KW - Development

KW - Runt homology domain

KW - Sclerotome

KW - Somite

KW - Transcription

KW - Transgene

KW - β-Galactosidase

UR - http://www.scopus.com/inward/record.url?scp=0036011256&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0036011256&partnerID=8YFLogxK

U2 - 10.1016/S0925-4773(02)00050-3

DO - 10.1016/S0925-4773(02)00050-3

M3 - Article

C2 - 12175505

AN - SCOPUS:0036011256

SN - 0925-4773

VL - 114

SP - 167

EP - 170

JO - Mechanisms of Development

JF - Mechanisms of Development

IS - 1-2

ER -

Activation of the bone-related Runx2/Cbfa1 promoter in mesenchymal condensations and developing chondrocytes of the axial skeleton

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this