Activation of protein kinase C is coupled to prostaglandin F synthesis in the ovary: studies in cultured swine granulosa cells

the technical assistance with, Paula Azimi, Diana Juchter and James Garmey of, Johannes D. Veldhuis, Lawrence M. Demers

Research output: Contribution to journalArticlepeer-review

13 Scopus citations

Abstract

We have investigated the role of phospholipid-sensitive calcium-dependent protein kinase (protein kinase C) in prostaglandin F synthesis by monolayer cultures of swine granulosa cells. In this system, specific phorbol ester derivatives known to activate protein kinase C significantly augmented the production of prostaglandin F. Phorbol ester in conjunction with the ionophore A23187 synergistically increased prostaglandin F production. These stimulatory actions were dose- and time-dependent, and could be abolished by the cyclooxygenase inhibitor, indomethacin, or the protein synthesis inhibitor, cycloheximide. Moreover, the rank order of potency of phorbol esters in enhancing prostaglandin F production was concordant with that demonstrated for activation of protein kinase C in the swine ovary. In addition, a nonphorbol stimulator of protein kinase C, l-octanoyl-2-acetylglycerol, also significantly enhanced prostaglandin F biosynthesis. The synthesis of immunoassayable prostaglandin F was confirmed by high-pressure liquid Chromatographie purification of this radiolabeled metabolite of [3H]arachidonic acid. Thus, the present studies indicate that the protein kinase C effector pathway in the swine granulosa cell is functionally coupled to prostaglandin F production.

Original languageEnglish (US)
Pages (from-to)249-254
Number of pages6
JournalMolecular and Cellular Endocrinology
Volume49
Issue number2-3
DOIs
StatePublished - Feb 1987

Keywords

  • (Activation)
  • (Ovary)
  • Granulosa cell
  • Prostaglandin F synthesis
  • Protein kinase C

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Endocrinology

Fingerprint Dive into the research topics of 'Activation of protein kinase C is coupled to prostaglandin F<sub>2α</sub> synthesis in the ovary: studies in cultured swine granulosa cells'. Together they form a unique fingerprint.

Cite this