We have investigated the role of phospholipid-sensitive calcium-dependent protein kinase (protein kinase C) in prostaglandin F2α synthesis by monolayer cultures of swine granulosa cells. In this system, specific phorbol ester derivatives known to activate protein kinase C significantly augmented the production of prostaglandin F2α. Phorbol ester in conjunction with the ionophore A23187 synergistically increased prostaglandin F2α production. These stimulatory actions were dose- and time-dependent, and could be abolished by the cyclooxygenase inhibitor, indomethacin, or the protein synthesis inhibitor, cycloheximide. Moreover, the rank order of potency of phorbol esters in enhancing prostaglandin F2α production was concordant with that demonstrated for activation of protein kinase C in the swine ovary. In addition, a nonphorbol stimulator of protein kinase C, l-octanoyl-2-acetylglycerol, also significantly enhanced prostaglandin F2α biosynthesis. The synthesis of immunoassayable prostaglandin F2α was confirmed by high-pressure liquid Chromatographie purification of this radiolabeled metabolite of [3H]arachidonic acid. Thus, the present studies indicate that the protein kinase C effector pathway in the swine granulosa cell is functionally coupled to prostaglandin F2α production.
- Granulosa cell
- Prostaglandin F synthesis
- Protein kinase C
ASJC Scopus subject areas
- Endocrinology, Diabetes and Metabolism