Activation of protein kinase C is coupled to prostaglandin E2 synthesis in swine granulosa cells

Johannes D Veldhuis, Lawrence M. Demers

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

We have examined the role of phospholipid-sensitive calcium-dependent protein kinase (protein kinase C) in prostaglandin E2 synthesis by monolayer cultures of swine granulosa cells. Specific phorbol ester derivatives known to activate protein kinase C significantly augmented the production of prostaglandin E2. These stimulatory actions were dose and time-dependent, and could be abolished by the cyclooxygenase inhibitor, indomethacin, or the protein synthesis inhibitor, cycloheximide. Moreover, the rank order of potency of phorbol esters in enhancing prostaglandin E2 production was concordant with that demonstrated for activation of protein kinase C. Phorbol ester in conjunction with the divalent cation ionophore, A23187, increased prostaglandin E2 production synergistically. In addition, a non-phorbol stimulator of protein kinase C, 1-octanoyl-2-acetylglycerol, also significantly enhanced prostaglandin E2 biosynthesis. The stimulated synthesis of prostaglandin E2 was confirmed by high-pressure liquid chromatographic purification of this radiolabeled metabolite of 3H-arachidonic acid, and by capillary gas chromatography high-resolution mass spectrometry. Thus, the present studies indicate that the protein kinase C effector pathway is functionally coupled to prostaglandin E2 production in the swine granulosa cell.

Original languageEnglish (US)
Pages (from-to)819-829
Number of pages11
JournalProstaglandins
Volume33
Issue number6
DOIs
StatePublished - 1987
Externally publishedYes

Fingerprint

Granulosa Cells
Dinoprostone
Protein Kinase C
Swine
Chemical activation
Phorbol Esters
Protein Synthesis Inhibitors
Cyclooxygenase Inhibitors
Biosynthesis
Ionophores
Divalent Cations
Calcimycin
Cycloheximide
Metabolites
Arachidonic Acid
Indomethacin
Gas chromatography
Gas Chromatography
Purification
Mass spectrometry

ASJC Scopus subject areas

  • Biochemistry
  • Endocrinology

Cite this

Activation of protein kinase C is coupled to prostaglandin E2 synthesis in swine granulosa cells. / Veldhuis, Johannes D; Demers, Lawrence M.

In: Prostaglandins, Vol. 33, No. 6, 1987, p. 819-829.

Research output: Contribution to journalArticle

Veldhuis, Johannes D ; Demers, Lawrence M. / Activation of protein kinase C is coupled to prostaglandin E2 synthesis in swine granulosa cells. In: Prostaglandins. 1987 ; Vol. 33, No. 6. pp. 819-829.
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