Activation of hindbrain neurons is mediated by portal-mesenteric vein glucosensors during slow-onset hypoglycemia

MaryAnn Bohland, Aleksey V Matveyenko, Maziyar Saberi, Arshad M. Khan, Alan G. Watts, Casey M. Donovan

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

Hypoglycemic detection at the portal-mesenteric vein (PMV) appears mediated by spinal afferents and is critical for the counter-regulatory response (CRR) to slow-onset, but not rapid-onset, hypoglycemia. Since rapid-onset hypoglycemia induces Fos protein expression in discrete brain regions, we hypothesized that denervation of the PMV or lesioning spinal afferents would suppress Fos expression in the dorsal medulla during slow-onset hypoglycemia, revealing a central nervous system reliance on PMV glucosensors. Rats undergoing PMV deafferentation via capsaicin, celiac-superior mesenteric ganglionectomy (CSMG), or total subdiaphragmatic vagotomy (TSV) were exposed to hyperinsulinemic- hypoglycemic clamps where glycemia was lowered slowly over 60-75 min. In response to hypoglycemia, control animals demonstrated a robust CRR along with marked Fos expression in the area postrema, nucleus of the solitary tract, and dorsal motor nucleus of the vagus. Fos expression was suppressed by 65-92% in capsaicin-treated animals, as was epineph-rine (74%), norepinephrine (33%), and glucagon (47%). CSMG also suppressed Fos expression and CRR during slow-onset hypoglycemia, whereas TSV failed to impact either. In contrast, CSMG failed to impact upon Fos expression or the CRR during rapid-onset hypoglyce-mia. Peripheral glucosensory input from the PMV is therefore required for activation of hindbrain neurons and the full CRR during slow-onset hypoglycemia.

Original languageEnglish (US)
Pages (from-to)2866-2875
Number of pages10
JournalDiabetes
Volume63
Issue number8
DOIs
StatePublished - 2014
Externally publishedYes

Fingerprint

Mesenteric Veins
Rhombencephalon
Portal Vein
Hypoglycemia
Ganglionectomy
Neurons
Abdomen
Vagotomy
Capsaicin
Hypoglycemic Agents
Area Postrema
Solitary Nucleus
Denervation
Glucagon
Norepinephrine
Central Nervous System
Brain
Proteins

ASJC Scopus subject areas

  • Internal Medicine
  • Endocrinology, Diabetes and Metabolism

Cite this

Activation of hindbrain neurons is mediated by portal-mesenteric vein glucosensors during slow-onset hypoglycemia. / Bohland, MaryAnn; Matveyenko, Aleksey V; Saberi, Maziyar; Khan, Arshad M.; Watts, Alan G.; Donovan, Casey M.

In: Diabetes, Vol. 63, No. 8, 2014, p. 2866-2875.

Research output: Contribution to journalArticle

Bohland, MaryAnn ; Matveyenko, Aleksey V ; Saberi, Maziyar ; Khan, Arshad M. ; Watts, Alan G. ; Donovan, Casey M. / Activation of hindbrain neurons is mediated by portal-mesenteric vein glucosensors during slow-onset hypoglycemia. In: Diabetes. 2014 ; Vol. 63, No. 8. pp. 2866-2875.
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abstract = "Hypoglycemic detection at the portal-mesenteric vein (PMV) appears mediated by spinal afferents and is critical for the counter-regulatory response (CRR) to slow-onset, but not rapid-onset, hypoglycemia. Since rapid-onset hypoglycemia induces Fos protein expression in discrete brain regions, we hypothesized that denervation of the PMV or lesioning spinal afferents would suppress Fos expression in the dorsal medulla during slow-onset hypoglycemia, revealing a central nervous system reliance on PMV glucosensors. Rats undergoing PMV deafferentation via capsaicin, celiac-superior mesenteric ganglionectomy (CSMG), or total subdiaphragmatic vagotomy (TSV) were exposed to hyperinsulinemic- hypoglycemic clamps where glycemia was lowered slowly over 60-75 min. In response to hypoglycemia, control animals demonstrated a robust CRR along with marked Fos expression in the area postrema, nucleus of the solitary tract, and dorsal motor nucleus of the vagus. Fos expression was suppressed by 65-92{\%} in capsaicin-treated animals, as was epineph-rine (74{\%}), norepinephrine (33{\%}), and glucagon (47{\%}). CSMG also suppressed Fos expression and CRR during slow-onset hypoglycemia, whereas TSV failed to impact either. In contrast, CSMG failed to impact upon Fos expression or the CRR during rapid-onset hypoglyce-mia. Peripheral glucosensory input from the PMV is therefore required for activation of hindbrain neurons and the full CRR during slow-onset hypoglycemia.",
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