TY - JOUR
T1 - Activation of EVI1 gene expression in human acute myelogenous leukemias by translocations spanning 300-400 kilobases on chromosome band 3q26
AU - Morishita, Kazuhiro
AU - Parganas, Evan
AU - Willman, Cheryl L.
AU - Whittaker, Michael H.
AU - Drabkin, Harry
AU - Oval, John
AU - Taetle, Raymond
AU - Valentine, Marcus B.
AU - Ihle, James N.
PY - 1992
Y1 - 1992
N2 - Retroviral activation of Evi-1 gene expression is one of the most common transforming events in murine myeloid leukemias. To evaluate the role of the EVI1 gene in human acute myelogenous leukemia (AML), leukemic blasts or cell lines from 116 patients were examined. In eight patients the EVI1 gene was expressed and all but one had cytogenetically detectable translocations of chromosome 3q26 where the EVI1 gene has been localized. To identify breakpoints, a restriction map that spans 1700 kilobases (kb) of the EVI1 locus was developed by pulsed-field gel electrophoresis. In one case, t(3;3)(q21;q26), a rearrangement was localized to 170-330 kb 5′ of the gene. In a second case, t(3;3)(q21;q26), there was a rearrangement 13 kb 5′ of the gene. This rearrangement was cloned and shown to be due to the fusion of sequences from 3q21-22 with the EVI1 locus. In the third case, ins(3)-(q21q25q27), there was a rearrangement that mapped 150 kb downstream from the 5′ end of the gene.
AB - Retroviral activation of Evi-1 gene expression is one of the most common transforming events in murine myeloid leukemias. To evaluate the role of the EVI1 gene in human acute myelogenous leukemia (AML), leukemic blasts or cell lines from 116 patients were examined. In eight patients the EVI1 gene was expressed and all but one had cytogenetically detectable translocations of chromosome 3q26 where the EVI1 gene has been localized. To identify breakpoints, a restriction map that spans 1700 kilobases (kb) of the EVI1 locus was developed by pulsed-field gel electrophoresis. In one case, t(3;3)(q21;q26), a rearrangement was localized to 170-330 kb 5′ of the gene. In a second case, t(3;3)(q21;q26), there was a rearrangement 13 kb 5′ of the gene. This rearrangement was cloned and shown to be due to the fusion of sequences from 3q21-22 with the EVI1 locus. In the third case, ins(3)-(q21q25q27), there was a rearrangement that mapped 150 kb downstream from the 5′ end of the gene.
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U2 - 10.1073/pnas.89.9.3937
DO - 10.1073/pnas.89.9.3937
M3 - Article
C2 - 1570317
AN - SCOPUS:0026503441
SN - 0027-8424
VL - 89
SP - 3937
EP - 3941
JO - Proceedings of the National Academy of Sciences of the United States of America
JF - Proceedings of the National Academy of Sciences of the United States of America
IS - 9
ER -