Activation of endothelial nitric oxide synthase is critical for erythropoietin-induced mobilization of progenitor cells

A. V R Santhanam, Livius V. d'Uscio, Timothy E. Peterson, Zvonimir S Katusic

Research output: Contribution to journalArticle

41 Citations (Scopus)

Abstract

The present study aimed to define the ability of erythropoietin (EPO) to mobilize hematopoietic stem cells (c-kit+/sca-1+/lin-1-; KSL-cells) and hematopoietic progenitor cells (CD34+ cells), including vascular endothelial growth factor receptor 2 expressing hematopoietic progenitor cells (CD34+/Flk-1+ cells). We also sought to determine the role of endothelial nitric oxide synthase (eNOS) in EPO-induced mobilization. Wild type (WT) and eNOS-/- mice were injected bi-weekly with recombinant erythropoietin (EPO, 1000 U/kg, s.c.) for 14 days. EPO increased the number of KSL, CD34+, CD34+/Flk-1+ cells in circulating blood of wild type mice. These effects of EPO were abolished in eNOS-/- mice. Our results demonstrate that, EPO stimulates mobilization of hematopoietic stem and progenitor cells. This effect of EPO is critically dependent on activation of eNOS.

Original languageEnglish (US)
Pages (from-to)1451-1455
Number of pages5
JournalPeptides
Volume29
Issue number8
DOIs
StatePublished - Aug 2008

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Nitric Oxide Synthase Type III
Erythropoietin
Stem Cells
Chemical activation
Hematopoietic Stem Cells
Vascular Endothelial Growth Factor Receptor-2
Stem cells
Blood

ASJC Scopus subject areas

  • Biochemistry
  • Endocrinology
  • Physiology
  • Cellular and Molecular Neuroscience

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Activation of endothelial nitric oxide synthase is critical for erythropoietin-induced mobilization of progenitor cells. / Santhanam, A. V R; d'Uscio, Livius V.; Peterson, Timothy E.; Katusic, Zvonimir S.

In: Peptides, Vol. 29, No. 8, 08.2008, p. 1451-1455.

Research output: Contribution to journalArticle

Santhanam, A. V R ; d'Uscio, Livius V. ; Peterson, Timothy E. ; Katusic, Zvonimir S. / Activation of endothelial nitric oxide synthase is critical for erythropoietin-induced mobilization of progenitor cells. In: Peptides. 2008 ; Vol. 29, No. 8. pp. 1451-1455.
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