Actin-binding proteins coronin-1a and IBA-1 are effective microglial markers for immunohistochemistry

Zeshan Ahmed, Gerry Shaw, Ved P. Sharma, Cui Yang, Eileen McGowan, Dennis W Dickson

Research output: Contribution to journalArticle

132 Citations (Scopus)

Abstract

This study identifies the actin-binding protein, coronin-1a, as a novel and effective immunohistochemical marker for microglia in both cell cultures and in formaldehyde-fixed, paraffin-embedded tissue. Antibodies to coronin-1a effectively immunostained microglia in human, monkey, horse, rat, and mouse tissues, even in tissues stored for long periods of time. The identity of coronin-1a-immunoreactive cells as microglia was confirmed using double immunolabeling with cell type-specific markers as well as by morphological features and the distribution of immunoreactive cells. These properties are shared by another actin-binding protein, IBA-1. Unlike IBA-1, coronin-1a immunoreactivity was also detected in lymphocytes and certain other hematopoietic cells. The results indicate that both coronin-1a and IBA-1 are robust markers for microglia that can be used in routinely processed tissue of humans and animals. Because both coronin-1a and IBA-1 are actin-binding proteins that play a role in rearrangement of the membrane cytoskeleton, it suggests that these proteins are critical to dynamic properties of microglia.

Original languageEnglish (US)
Pages (from-to)687-700
Number of pages14
JournalJournal of Histochemistry and Cytochemistry
Volume55
Issue number7
DOIs
StatePublished - Jul 2007

Fingerprint

Microfilament Proteins
Microglia
Immunohistochemistry
Cytoskeleton
Paraffin
Formaldehyde
Horses
Haplorhini
coronin
Cell Culture Techniques
Lymphocytes
Membranes
Antibodies
Proteins

Keywords

  • Coronin-1a
  • IBA-1
  • Immunohistochemistry
  • Microglia

ASJC Scopus subject areas

  • Anatomy
  • Cell Biology

Cite this

Actin-binding proteins coronin-1a and IBA-1 are effective microglial markers for immunohistochemistry. / Ahmed, Zeshan; Shaw, Gerry; Sharma, Ved P.; Yang, Cui; McGowan, Eileen; Dickson, Dennis W.

In: Journal of Histochemistry and Cytochemistry, Vol. 55, No. 7, 07.2007, p. 687-700.

Research output: Contribution to journalArticle

Ahmed, Zeshan ; Shaw, Gerry ; Sharma, Ved P. ; Yang, Cui ; McGowan, Eileen ; Dickson, Dennis W. / Actin-binding proteins coronin-1a and IBA-1 are effective microglial markers for immunohistochemistry. In: Journal of Histochemistry and Cytochemistry. 2007 ; Vol. 55, No. 7. pp. 687-700.
@article{a8a6ee68b4f24d20b7d1239131cab152,
title = "Actin-binding proteins coronin-1a and IBA-1 are effective microglial markers for immunohistochemistry",
abstract = "This study identifies the actin-binding protein, coronin-1a, as a novel and effective immunohistochemical marker for microglia in both cell cultures and in formaldehyde-fixed, paraffin-embedded tissue. Antibodies to coronin-1a effectively immunostained microglia in human, monkey, horse, rat, and mouse tissues, even in tissues stored for long periods of time. The identity of coronin-1a-immunoreactive cells as microglia was confirmed using double immunolabeling with cell type-specific markers as well as by morphological features and the distribution of immunoreactive cells. These properties are shared by another actin-binding protein, IBA-1. Unlike IBA-1, coronin-1a immunoreactivity was also detected in lymphocytes and certain other hematopoietic cells. The results indicate that both coronin-1a and IBA-1 are robust markers for microglia that can be used in routinely processed tissue of humans and animals. Because both coronin-1a and IBA-1 are actin-binding proteins that play a role in rearrangement of the membrane cytoskeleton, it suggests that these proteins are critical to dynamic properties of microglia.",
keywords = "Coronin-1a, IBA-1, Immunohistochemistry, Microglia",
author = "Zeshan Ahmed and Gerry Shaw and Sharma, {Ved P.} and Cui Yang and Eileen McGowan and Dickson, {Dennis W}",
year = "2007",
month = "7",
doi = "10.1369/jhc.6A7156.2007",
language = "English (US)",
volume = "55",
pages = "687--700",
journal = "Journal of Histochemistry and Cytochemistry",
issn = "0022-1554",
publisher = "Histochemical Society Inc.",
number = "7",

}

TY - JOUR

T1 - Actin-binding proteins coronin-1a and IBA-1 are effective microglial markers for immunohistochemistry

AU - Ahmed, Zeshan

AU - Shaw, Gerry

AU - Sharma, Ved P.

AU - Yang, Cui

AU - McGowan, Eileen

AU - Dickson, Dennis W

PY - 2007/7

Y1 - 2007/7

N2 - This study identifies the actin-binding protein, coronin-1a, as a novel and effective immunohistochemical marker for microglia in both cell cultures and in formaldehyde-fixed, paraffin-embedded tissue. Antibodies to coronin-1a effectively immunostained microglia in human, monkey, horse, rat, and mouse tissues, even in tissues stored for long periods of time. The identity of coronin-1a-immunoreactive cells as microglia was confirmed using double immunolabeling with cell type-specific markers as well as by morphological features and the distribution of immunoreactive cells. These properties are shared by another actin-binding protein, IBA-1. Unlike IBA-1, coronin-1a immunoreactivity was also detected in lymphocytes and certain other hematopoietic cells. The results indicate that both coronin-1a and IBA-1 are robust markers for microglia that can be used in routinely processed tissue of humans and animals. Because both coronin-1a and IBA-1 are actin-binding proteins that play a role in rearrangement of the membrane cytoskeleton, it suggests that these proteins are critical to dynamic properties of microglia.

AB - This study identifies the actin-binding protein, coronin-1a, as a novel and effective immunohistochemical marker for microglia in both cell cultures and in formaldehyde-fixed, paraffin-embedded tissue. Antibodies to coronin-1a effectively immunostained microglia in human, monkey, horse, rat, and mouse tissues, even in tissues stored for long periods of time. The identity of coronin-1a-immunoreactive cells as microglia was confirmed using double immunolabeling with cell type-specific markers as well as by morphological features and the distribution of immunoreactive cells. These properties are shared by another actin-binding protein, IBA-1. Unlike IBA-1, coronin-1a immunoreactivity was also detected in lymphocytes and certain other hematopoietic cells. The results indicate that both coronin-1a and IBA-1 are robust markers for microglia that can be used in routinely processed tissue of humans and animals. Because both coronin-1a and IBA-1 are actin-binding proteins that play a role in rearrangement of the membrane cytoskeleton, it suggests that these proteins are critical to dynamic properties of microglia.

KW - Coronin-1a

KW - IBA-1

KW - Immunohistochemistry

KW - Microglia

UR - http://www.scopus.com/inward/record.url?scp=34250796207&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=34250796207&partnerID=8YFLogxK

U2 - 10.1369/jhc.6A7156.2007

DO - 10.1369/jhc.6A7156.2007

M3 - Article

C2 - 17341475

AN - SCOPUS:34250796207

VL - 55

SP - 687

EP - 700

JO - Journal of Histochemistry and Cytochemistry

JF - Journal of Histochemistry and Cytochemistry

SN - 0022-1554

IS - 7

ER -