Actin-binding proteins coronin-1a and IBA-1 are effective microglial markers for immunohistochemistry

Zeshan Ahmed, Gerry Shaw, Ved P. Sharma, Cui Yang, Eileen McGowan, Dennis W. Dickson

Research output: Contribution to journalArticle

138 Scopus citations

Abstract

This study identifies the actin-binding protein, coronin-1a, as a novel and effective immunohistochemical marker for microglia in both cell cultures and in formaldehyde-fixed, paraffin-embedded tissue. Antibodies to coronin-1a effectively immunostained microglia in human, monkey, horse, rat, and mouse tissues, even in tissues stored for long periods of time. The identity of coronin-1a-immunoreactive cells as microglia was confirmed using double immunolabeling with cell type-specific markers as well as by morphological features and the distribution of immunoreactive cells. These properties are shared by another actin-binding protein, IBA-1. Unlike IBA-1, coronin-1a immunoreactivity was also detected in lymphocytes and certain other hematopoietic cells. The results indicate that both coronin-1a and IBA-1 are robust markers for microglia that can be used in routinely processed tissue of humans and animals. Because both coronin-1a and IBA-1 are actin-binding proteins that play a role in rearrangement of the membrane cytoskeleton, it suggests that these proteins are critical to dynamic properties of microglia.

Original languageEnglish (US)
Pages (from-to)687-700
Number of pages14
JournalJournal of Histochemistry and Cytochemistry
Volume55
Issue number7
DOIs
StatePublished - Jul 1 2007

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Keywords

  • Coronin-1a
  • IBA-1
  • Immunohistochemistry
  • Microglia

ASJC Scopus subject areas

  • Anatomy
  • Histology

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