Actin and Arf1-dependent recruitment of a cortactin-dynamin complex to the Golgi regulates post-Golgi transport

Hong Cao, Shaun Weller, James D. Orth, Jing Chen, Bing Huang, Ji Long Chen, Mark Stamnes, Mark A. McNiven

Research output: Contribution to journalArticlepeer-review

137 Scopus citations

Abstract

Cortactin is an actin-binding protein that has recently been implicated in endocytosis. It binds directly to dynamin-2 (Dyn2), a large GTPase that mediates the formation of vesicles from the plasma membrane and the Golgi. Here we show that cortactin associates with the Golgi to regulate the actin- and Dyn2-dependent transport of cargo. Cortactin antibodies stain the Golgi apparatus, labelling peripheral buds and vesicles that are associated with the cisternae. Notably, in vitro or intact-cell experiments show that activation of Arf1 mediates the recruitment of actin, cortactin and Dyn2 to Golgi membranes. Furthermore, selective disruption of the cortactin-Dyn2 interaction significantly reduces the levels of Dyn2 at the Golgi and blocks the transit of nascent proteins from the trans-Golgi network, resulting in swollen and distended cisternae. These findings support the idea of an Arf1-activated recruitment of an actin, cortactin and Dyn2 complex that is essential for Golgi function.

Original languageEnglish (US)
Pages (from-to)483-492
Number of pages10
JournalNature Cell Biology
Volume7
Issue number5
DOIs
StatePublished - May 2005

ASJC Scopus subject areas

  • Cell Biology

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